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Protein

Alpha-amylase

Gene
N/A
Organism
Bacillus firmus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

On the 2D-gel the determined pI of this protein is: 5.0, its MW is: 155 kDa.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication

Kineticsi

Because of the indeterminate molecular weight of starch, an approximate KM value of 0.975 mg/ml was determined.1 Publication

      pH dependencei

      Optimum pH is 5.0.1 Publication

      Temperature dependencei

      Optimum temperature is 60 degrees Celsius.1 Publication

      GO - Molecular functioni

      GO - Biological processi

      Keywordsi

      Molecular functionGlycosidase, Hydrolase
      Biological processCarbohydrate metabolism, Polysaccharide degradation

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Alpha-amylase1 Publication (EC:3.2.1.11 Publication)
      Alternative name(s):
      1,4-alpha-D-glucan glucanohydrolaseBy similarity
      OrganismiBacillus firmus
      Taxonomic identifieri1399 [NCBI]
      Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00004241761 – ›10Alpha-amylase›10

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the glycosyl hydrolase 13 family.Curated

      Sequencei

      Sequence statusi: Fragment.

      Length:10
      Mass (Da):1,150
      Last modified:October 16, 2013 - v1
      Checksum:iD9F28FC2C3333862
      GO

      Experimental Info

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Non-terminal residuei101 Publication1

      Cross-referencesi

      3D structure databases

      ModBaseiSearch...
      MobiDBiSearch...

      Protocols and materials databases

      Structural Biology KnowledgebaseSearch...

      Family and domain databases

      ProtoNetiSearch...

      Entry informationi

      Entry nameiAMY1_BACFI
      AccessioniPrimary (citable) accession number: C0HJE4
      Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
      Last sequence update: October 16, 2013
      Last modified: September 27, 2017
      This is version 4 of the entry and version 1 of the sequence. See complete history.
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Direct protein sequencing

      Documents

      1. SIMILARITY comments
        Index of protein domains and families
      2. Glycosyl hydrolases
        Classification of glycosyl hydrolase families and list of entries
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