UniProtKB - B9WZX5 (FTMF_ASPFM)
Verruculogen synthase
ftmOx1
Functioni
Verruculogen synthase; part of the gene cluster that mediates the biosynthesis of fumitremorgins, indole alkaloids that carry not only intriguing chemical structures, but also interesting biological and pharmacological activities (PubMed:19763315, PubMed:23649274).
The biosynthesis of fumitremorgin-type alkaloids begins by condensation of the two amino acids L-tryptophan and L-proline to brevianamide F, catalyzed by the non-ribosomal peptide synthetase ftmA (PubMed:16755625).
Brevianamide F is then prenylated by the prenyltransferase ftmPT1/ftmB in the presence of dimethylallyl diphosphate, resulting in the formation of tryprostatin B (PubMed:16000710, PubMed:21105662, PubMed:23090579).
The three cytochrome P450 monooxygenases, ftmP450-1/ftmC, ftmP450-2/ftmE and ftmP450-3/FtmG, are responsible for the conversion of tryprostatin B to 6-hydroxytryprostatin B, tryprostatin A to fumitremorgin C and fumitremorgin C to 12,13-dihydroxyfumitremorgin C, respectively (PubMed:19226505).
The putative methyltransferase ftmMT/ftmD is expected for the conversion of 6-hydroxytryprostatin B to tryprostatin A (Probable). FtmPT2/FtmH catalyzes the prenylation of 12,13-dihydroxyfumitre-morgin C in the presence of dimethylallyl diphosphate, resulting in the formation of fumitremorgin B (PubMed:18683158).
Fumitremorgin B is further converted to verruculogen by ftmOx1/ftmF via the insertion of an endoperoxide bond between the two prenyl moieties (PubMed:19763315).
In some fungal species, verruculogen is further converted to fumitremorgin A, but the enzymes involved in this step have not been identified yet (Probable).
Curated8 PublicationsCatalytic activityi
- 2-oxoglutarate + AH2 + fumitremorgin B + 2 O2 = A + CO2 + H2O + succinate + verruculogen1 PublicationEC:1.14.11.381 Publication
Cofactori
: Mycotoxin biosynthesis Pathwayi
This protein is involved in Mycotoxin biosynthesis.2 PublicationsView all proteins of this organism that are known to be involved in Mycotoxin biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 68 | By similarity | 1 |
GO - Molecular functioni
- dioxygenase activity Source: UniProtKB-KW
GO - Biological processi
- verruculogen biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Alkaloid metabolism, Virulence |
Ligand | Iron |
Names & Taxonomyi
Protein namesi | Recommended name: Verruculogen synthase1 Publication (EC:1.14.11.381 Publication)Alternative name(s): Fumitremorgin biosynthesis protein F1 Publication |
Gene namesi | Name:ftmOx11 Publication Synonyms:ftmF1 Publication |
Organismi | Neosartorya fumigata (Aspergillus fumigatus) |
Taxonomic identifieri | 746128 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424134 | 1 – 291 | Verruculogen synthaseAdd BLAST | 291 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR008775, Phytyl_CoA_dOase |
Pfami | View protein in Pfam PF05721, PhyH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTVDSKPQLQ RLAADADVDR MCRLLEEDGA FILKGLLPFD VVESFNRELD
60 70 80 90 100
VQMAIPPPKG ERLLADKYPP HFKYVPNVAT TCPTFRNTIL INPVIHAICE
110 120 130 140 150
AYFQRTGDYW LSAAFLREIE SGMPAQPFHR DDATHPLMHY QPLEAPPISL
160 170 180 190 200
SVIFPLTEFT EENGATEVIL GSHRWTEVGT PERDQAVLAT MDPGDVLIVR
210 220 230 240 250
QRVVHAGGGN RTTAGKPRRV VLAYFNSVQL TPFETYRTMP REMVESMTVL
260 270 280 290
GQRMLGWRTM KPSDPNIVGI NLIDDKRLEN VLQLKAADSP A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB436628 Genomic DNA Translation: BAH24000.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB436628 Genomic DNA Translation: BAH24000.1 |
3D structure databases
AlphaFoldDBi | B9WZX5 |
SMRi | B9WZX5 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR008775, Phytyl_CoA_dOase |
Pfami | View protein in Pfam PF05721, PhyH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FTMF_ASPFM | |
Accessioni | B9WZX5Primary (citable) accession number: B9WZX5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | April 14, 2009 | |
Last modified: | May 25, 2022 | |
This is version 38 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families