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UniProtKB - B9WZX2 (FTMD_ASPFM)
Protein
6-hydroxytryprostatin B O-methyltransferase
Gene
ftmMT
Organism
Neosartorya fumigata (Aspergillus fumigatus)
Status
Functioni
S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes the conversion of 6-hydroxytryprostatin B to tryprostatin A in the biosynthesis of indole alkaloids such as fumitremorgins and verruculogen.
1 PublicationCatalytic activityi
- 6-hydroxytryprostatin B + S-adenosyl-L-methionine = H+ + S-adenosyl-L-homocysteine + tryprostatin A1 PublicationEC:2.1.1.2931 Publication
Kineticsi
kcat is 27 sec(-1) with 6-hydroxytryprostatin B as substrate. kcat is 28 sec(-1) with S-adenosyl-L-methionine as substrate.
- KM=11 µM for S-adenosyl-L-methionine1 Publication
- KM=21 µM for 6-hydroxytryprostatin B1 Publication
: Alkaloid biosynthesis Pathwayi
This protein is involved in Alkaloid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in Alkaloid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 201 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Sitei | 202 | Required for binding 6-hydroxytryprostatin B | 1 | |
Active sitei | 244 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- O-methyltransferase activity Source: InterPro
GO - Biological processi
- alkaloid metabolic process Source: UniProtKB-KW
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Alkaloid metabolism |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.293, 508 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-hydroxytryprostatin B O-methyltransferase (EC:2.1.1.293)Alternative name(s): Fumitremorgin biosynthesis protein D |
Gene namesi | Name:ftmMT Synonyms:ftmD |
Organismi | Neosartorya fumigata (Aspergillus fumigatus) |
Taxonomic identifieri | 746128 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424129 | 1 – 342 | 6-hydroxytryprostatin B O-methyltransferaseAdd BLAST | 342 |
Interactioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.PROSITE-ProRule annotation
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR43712, PTHR43712, 1 hit |
Pfami | View protein in Pfam PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
i Sequence
Sequence statusi: Complete.
B9WZX2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQAVDIGTI QTLIRLEVPD QVPLTGSIPY DALVKKLKTP VDPELLQRLI
60 70 80 90 100
RFTRLVGFLD EDAEGAVKHS PMSAIFVNDP DTAGQARFMA DFGIRPCSFI
110 120 130 140 150
YESIKLDPSG EAARQGPLAL MAREPGAREG PTFFEVLEKD PVNRKRWHDG
160 170 180 190 200
MAVHNDSMVR HVAGAYDWGT VQSLVDIGGS EGHVAAVIVN AFPHIQITVQ
210 220 230 240 250
DRPEIIEKAR QRGDRHPNII FEEHDFFTPQ PRIADAYFLR LILHDWNDAD
260 270 280 290 300
CTRIVRQISS ALRPGARLLI MDAVLPEPGE GSLQSERRLR RSDIGMYTLF
310 320 330 340
SAKERSLAQM RRLVEDCDSR LRFEKLYTPP GSHASMLSWI CE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB436628 Genomic DNA Translation: BAH23997.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB436628 Genomic DNA Translation: BAH23997.1 |
3D structure databases
SMRi | B9WZX2 |
ModBasei | Search... |
Enzyme and pathway databases
BRENDAi | 2.1.1.293, 508 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR43712, PTHR43712, 1 hit |
Pfami | View protein in Pfam PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FTMD_ASPFM | |
Accessioni | B9WZX2Primary (citable) accession number: B9WZX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | April 14, 2009 | |
Last modified: | February 23, 2022 | |
This is version 43 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families