UniProtKB - B9WZX0 (FTMA_ASPFM)
Nonribosomal peptide synthetase 13
NRPS13
Functioni
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of fumitremorgins, indole alkaloids that carry not only intriguing chemical structures, but also interesting biological and pharmacological activities (PubMed:16755625, PubMed:23649274).
The biosynthesis of fumitremorgin-type alkaloids begins by condensation of the two amino acids L-tryptophan and L-proline to brevianamide F, catalyzed by the non-ribosomal peptide synthetase ftmA (PubMed:16755625, PubMed:17464044).
Brevianamide F is then prenylated by the prenyltransferase ftmPT1/ftmB in the presence of dimethylallyl diphosphate, resulting in the formation of tryprostatin B (PubMed:16000710, PubMed:21105662, PubMed:23090579).
The three cytochrome P450 monooxygenases, ftmP450-1/ftmC, ftmP450-2/ftmE and ftmP450-3/FtmG, are responsible for the conversion of tryprostatin B to 6-hydroxytryprostatin B, tryprostatin A to fumitremorgin C and fumitremorgin C to 12,13-dihydroxyfumitremorgin C, respectively (PubMed:19226505).
The putative methyltransferase ftmMT/ftmD is expected for the conversion of 6-hydroxytryprostatin B to tryprostatin A (Probable). FtmPT2/FtmH catalyzes the prenylation of 12,13-dihydroxyfumitre-morgin C in the presence of dimethylallyl diphosphate, resulting in the formation of fumitremorgin B (PubMed:18683158).
Fumitremorgin B is further converted to verruculogen by ftmOx1/ftmF via the insertion of an endoperoxide bond between the two prenyl moieties (PubMed:19763315).
In some fungal species, verruculogen is further converted to fumitremorgin A, but the enzymes involved in this step have not been identified yet (Probable).
Curated9 PublicationsCatalytic activityi
: Mycotoxin biosynthesis Pathwayi
This protein is involved in Mycotoxin biosynthesis.2 PublicationsView all proteins of this organism that are known to be involved in Mycotoxin biosynthesis.
GO - Molecular functioni
- isomerase activity Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
GO - Biological processi
- verruculogen biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Isomerase, Ligase |
Biological process | Alkaloid metabolism, Virulence |
Names & Taxonomyi
Protein namesi | Recommended name: Nonribosomal peptide synthetase 131 Publication (EC:6.3.3.-1 Publication)Alternative name(s): Brevianamide F synthase1 Publication Fumitremorgin biosynthesis protein A1 Publication |
Gene namesi | Name:NRPS131 Publication Synonyms:ftmA1 Publication |
Organismi | Neosartorya fumigata (Aspergillus fumigatus) |
Taxonomic identifieri | 746128 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424109 | 1 – 2211 | Nonribosomal peptide synthetase 13Add BLAST | 2211 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 631 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 | |
Modified residuei | 1714 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinExpressioni
Inductioni
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 594 – 671 | Carrier 1PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 1677 – 1756 | Carrier 2PROSITE-ProRule annotationAdd BLAST | 80 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 76 – 475 | Adenylation 1Sequence analysis1 PublicationAdd BLAST | 400 | |
Regioni | 710 – 975 | Condensation 1Sequence analysis1 PublicationAdd BLAST | 266 | |
Regioni | 1169 – 1563 | Adenylation 2Sequence analysis1 PublicationAdd BLAST | 395 | |
Regioni | 1814 – 2069 | Condensation 2Sequence analysis1 PublicationAdd BLAST | 256 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatFamily and domain databases
Gene3Di | 1.10.1200.10, 2 hits 3.30.300.30, 2 hits 3.30.559.10, 2 hits 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR036736, ACP-like_sf IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00501, AMP-binding, 2 hits PF00668, Condensation, 2 hits PF00550, PP-binding, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 2 hits PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAMALAVGAP SEQRGNLPYN TLKDGPTVDS MKATTDGKNG QGESAFWDTC
60 70 80 90 100
VHTVFREHCQ RAPNSPAVNA WDGSFTYAEL DSLSDAIASV LILSGVGPES
110 120 130 140 150
IIPIYMQKSR WTTVAILGVL KSGGAFTLLD PSHPRSRVEE ISKEIQARFI
160 170 180 190 200
LTSEKLSKQC LEMFSVLVVE HLSRACLPSP GQAGHTRSRP ENAAYIAFTS
210 220 230 240 250
GSTGKPKGIV IEHRSYCSGA RSHLKVFGID STSRVLQFAS YAFDVSIMET
260 270 280 290 300
LSTLMAGGCL CVMSESERSD PNLFVVSYKN LRISHCFMTP SFARTVPWTE
310 320 330 340 350
CCNPPPTLIV GGELMRPSDA RAYKEMGIRC MNAYGPAECS VNVSVQSRVE
360 370 380 390 400
AAVDPRNIGY TTGATAWIIS PENPEELMPT GTVGELLVEG PIVGRGYLND
410 420 430 440 450
PKATRQAFID TPAWLRRHRK GTSYQHRVYR TGDLASLDSI TGALLLHGRK
460 470 480 490 500
DAQVKIRGQR VELPDIEHHL QLTLPNDNAE VIVEKVTFSD DGSEKLIAFV
510 520 530 540 550
LVRPSNTDSV IGNTGDRLFL APQSQIMEQF AISKKHLQTH LPSYMVPDIF
560 570 580 590 600
IPISTLPQTA SGKTDRKALR TRAAALSRRD VQCFLLSPAG GKRPPSTPKE
610 620 630 640 650
ATIRSLYSNV LNLPIDLIGM DDTFLRLGGD SLQAIRLVAA ARAAGLILHA
660 670 680 690 700
KDILSSQSTL AEQSKRAGLI QTIDRTWESS PPFALLHGPT RHAIVDLAQK
710 720 730 740 750
QCRVPSNLIE DIYPCTALQE GMFITSLKHP GMYTGQIIFD IPDRMELPRL
760 770 780 790 800
KAAWLSVVSE NAALRTRIIE THEGLMQAVI VDDFVWEEET DEMLLSSDGE
810 820 830 840 850
ALEITKIGVP LVRFRYRPRH RQLMMTIHHS IWDGWSLRLV HEQLQRAYIG
860 870 880 890 900
RDLLPSTSYR SFIQYTQELP GADEFWASEL AGVNAPIFPT LPSGNYRPRV
910 920 930 940 950
NASHRHVVRN LASTGKEEHT AATYIHLAWS LLVAHYTDAD ETVYGVTVNG
960 970 980 990 1000
RSADVPGIEN IVGPTIATVP TRIRVNEEDT VEMALDHVQD ALARMIPYEQ
1010 1020 1030 1040 1050
AGLQRISRCS RDASEACRFQ NLLIIEAPTD SDVDCEKNEA GNFSIIGGTT
1060 1070 1080 1090 1100
QTGMDYTAFS SYAMMLVFRT SANKSAISFD ITYDAQVIGH DEVERMAHQF
1110 1120 1130 1140 1150
EHVLRHIYTL ATGRIGDISF IGPRDIEQVQ QWNSSMPPAD NRFLQELIFA
1160 1170 1180 1190 1200
QCSRRPQASA IISWDGSWTY RELWAHSSFF ARQLQRYGVT RGTPVAVCLD
1210 1220 1230 1240 1250
RSRWSIAVIL GVLLARGTCV LIDLLAPRQR VRDILQIAGT GILVHSHATA
1260 1270 1280 1290 1300
TLTSGLCPTV INVSFLAAQS DSSQPEFPFT LETWGGTPED LAFIIFTSGS
1310 1320 1330 1340 1350
TGHPKGIEMP HRTLSTSISH HSAGMRVTSS SRVLHFSSYA FDVSIYEIFT
1360 1370 1380 1390 1400
TLAAGGTICV PSEFDRMNNL AGFIQDTQVN WAFLTPSTAR SLNPADVPLL
1410 1420 1430 1440 1450
TTLVLGGEAV THESVEVWAK GRSLINGYGP AEATICGVGN IPEAGWKSGV
1460 1470 1480 1490 1500
VGRIIGGLGW VTVPSDPNRL AAVGAVGELL LEGPFLARGY LNLPEVTKAA
1510 1520 1530 1540 1550
FIDPPSWRTR IPAPSPYSFL YRTGDLVRYQ PDGSIQYVGR KDSRVKLRGQ
1560 1570 1580 1590 1600
LVDLGAVEAS VMRVYPAAGQ VVADVLVSEN TARLIAMVKL GPSVTENHDG
1610 1620 1630 1640 1650
PMFAAPDLVF NEAAASIQAR LRAIVPAYMV PSMFIPLRHI PRTLTGKTDR
1660 1670 1680 1690 1700
RRLRDKILSL SHSDLQRYMM SSSTKTPMSD DNERRLQEIW AEVLQLPCEA
1710 1720 1730 1740 1750
IGREDSFLSL GGESLATMKM VALARRVGFM FAVTDVMNNT SLSTLARSRH
1760 1770 1780 1790 1800
LITEQAILTS SPSLSLPTIE GESLQEILRP LLNAGHIQGG NDIAAIHPVT
1810 1820 1830 1840 1850
AAQAFLVQRY PWSHFQFDLS GAVSPSKLQT ACTALMARFT ILRTVFVEHA
1860 1870 1880 1890 1900
GCLLQLVLRE VPNRVHEITT NEPLDDFCNS VCQQQQDVCV VNSTTLPTLF
1910 1920 1930 1940 1950
TLVSNRQLNR HRLLLRLAHA QYDLTTIPLI VQSLADEYNR TLRSGFSADF
1960 1970 1980 1990 2000
GYYLSHHKRQ NNDDRSHNFW KRYLSGSSMM STNQTADPTT VQERVFHVTG
2010 2020 2030 2040 2050
SCIIIPTSHP PDITIATAVK AAVCLVLAAR TGCTDIVIGQ TVDARCSSAD
2060 2070 2080 2090 2100
STLDQIVGPC TNYIPYRLSV CCSKTALEYL RSAQAQHTTC LRYSSLDLDQ
2110 2120 2130 2140 2150
IVAKCTSWPS STQFGYIVQH QDTGAELALT LGGDTTSLPM TSYGRVFPQG
2160 2170 2180 2190 2200
EVWIGSTPCS TGLRIDVIAL SAVLSQKDAQ TMAEEVGAAL EKLLGCGYRR
2210
LSHLIGNTFA T
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB436628 Genomic DNA Translation: BAH23995.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB436628 Genomic DNA Translation: BAH23995.1 |
3D structure databases
AlphaFoldDBi | B9WZX0 |
SMRi | B9WZX0 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 1.10.1200.10, 2 hits 3.30.300.30, 2 hits 3.30.559.10, 2 hits 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR036736, ACP-like_sf IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00501, AMP-binding, 2 hits PF00668, Condensation, 2 hits PF00550, PP-binding, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 2 hits PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | FTMA_ASPFM | |
Accessioni | B9WZX0Primary (citable) accession number: B9WZX0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | April 14, 2009 | |
Last modified: | May 25, 2022 | |
This is version 60 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families