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Protein

Biotin synthase

Gene

bioB

Organism
Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi77Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi80Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi117Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi149Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi209Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBES521460:G1GUU-2580-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Athe_2541
OrganismiCaldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) (Anaerocellum thermophilum)
Taxonomic identifieri521460 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor
Proteomesi
  • UP000007723 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812031 – 337Biotin synthaseAdd BLAST337

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi521460.Athe_2541.

Structurei

3D structure databases

ProteinModelPortaliB9MP50.
SMRiB9MP50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF. Bacteria.
COG0502. LUCA.
HOGENOMiHOG000239958.
KOiK01012.
OMAiADRFCMG.
OrthoDBiPOG091H01DF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR034416. BATS_domain_containing.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDG01060. BATS_domain_containing. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9MP50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNFLQSVQF VKEVEKKIIE YDKDIAFNEA IILYEIAKHD ADLVKNLAST
60 70 80 90 100
INQHYFKNTI ELCSIYPAKV GLCPQDCKFC SQSIHHSCLI EIKDLAALDE
110 120 130 140 150
VIEYLEYVIS FPIKRFCLVT SGEKLDDSEF EKILDIYSHI SKNYNILLCA
160 170 180 190 200
SLGFLTQERA KKLLKVGVVK YHNNLETSST YFKNICSTHT QQQKIETLKI
210 220 230 240 250
AKEAGLQICS GGIISMGEDM IERIKLAFEL RELDVDSVPI NILNPIKGTP
260 270 280 290 300
LEDIKIIDKN EIFITLALFR IVLPKKTILL AGGKENALGD MEKMAYECGV
310 320 330
NGCMVGNYLT TRGMGIREKI EMLESLDLKF QTNMHNN
Length:337
Mass (Da):38,251
Last modified:March 24, 2009 - v1
Checksum:i10933A43C84C67FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001393 Genomic DNA. Translation: ACM61609.1.
RefSeqiWP_015908859.1. NC_012034.1.

Genome annotation databases

EnsemblBacteriaiACM61609; ACM61609; Athe_2541.
GeneIDi31773896.
KEGGiate:Athe_2541.

Similar proteinsi

Entry informationi

Entry nameiBIOB_CALBD
AccessioniPrimary (citable) accession number: B9MP50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome