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Protein

Enolase

Gene

eno

Organism
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (CTN_0477)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateUniRule annotation1
Binding sitei168SubstrateUniRule annotation1
Active sitei209Proton donorUniRule annotation1
Metal bindingi246MagnesiumUniRule annotation1
Metal bindingi289MagnesiumUniRule annotation1
Binding sitei289SubstrateUniRule annotation1
Metal bindingi316MagnesiumUniRule annotation1
Binding sitei316SubstrateUniRule annotation1
Active sitei341Proton acceptorUniRule annotation1
Binding sitei341Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei392SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:CTN_1698
OrganismiThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Taxonomic identifieri309803 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000000445 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001330281 – 432EnolaseAdd BLAST432

Proteomic databases

PRIDEiB9KA91

Interactioni

Protein-protein interaction databases

STRINGi309803.CTN_1698

Structurei

3D structure databases

ProteinModelPortaliB9KA91
SMRiB9KA91
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 371Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
HOGENOMiHOG000072173
KOiK01689
OMAiEFMIIPV
OrthoDBiPOG091H02DK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

B9KA91-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEIVYVEIVD VRAREVLDSR GNPTVEAEVV LEDGTVGRAI VPSGASTGKF
60 70 80 90 100
EALEIRDKDK KRYHGKGVLK AVENVNETIA PALIGMNAFD QPLVDKTLIE
110 120 130 140 150
LDGTENKSKL GANAILAVSM AVARAAANYL GLPMYKYLGG VNAKVLPVPL
160 170 180 190 200
MNVINGGQHA DNNLDLQEFM IVPAGFDSFK EALRAGAEIF HTLKKILHDS
210 220 230 240 250
GHVTAVGDEG GFAPNLSSNE EAIKILIEAI EEAGYKPSEQ VFIALDCAAS
260 270 280 290 300
SFYDEEKGVY FVDGEEKSSE VLMGYYEELI AKYPIISIED PFAEEDWDAF
310 320 330 340 350
VEFTKRVGNK VQIVGDDLYV TNVKRLSKGI ELKATNSILI KLNQIGTVTE
360 370 380 390 400
TLDAVELAQK NNMTAIISHR SGESEDTFIA DLAVATNAGF IKTGSLSRSE
410 420 430
RIAKYNQLLR IEEELGKVAE FRGLSSFYSI KR
Length:432
Mass (Da):47,179
Last modified:March 24, 2009 - v1
Checksum:i631A84B75EB1560E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000916 Genomic DNA Translation: ACM23874.1

Genome annotation databases

EnsemblBacteriaiACM23874; ACM23874; CTN_1698
KEGGitna:CTN_1698

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000916 Genomic DNA Translation: ACM23874.1

3D structure databases

ProteinModelPortaliB9KA91
SMRiB9KA91
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309803.CTN_1698

Proteomic databases

PRIDEiB9KA91

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM23874; ACM23874; CTN_1698
KEGGitna:CTN_1698

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
HOGENOMiHOG000072173
KOiK01689
OMAiEFMIIPV
OrthoDBiPOG091H02DK

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00187

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENO_THENN
AccessioniPrimary (citable) accession number: B9KA91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 10, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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