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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (Arad_1335)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71ShikimateUniRule annotation1
Active sitei75Proton acceptorUniRule annotation1
Binding sitei87NADPUniRule annotation1
Binding sitei96ShikimateUniRule annotation1
Binding sitei111ShikimateUniRule annotation1
Binding sitei225NADP; via carbonyl oxygenUniRule annotation1
Binding sitei227ShikimateUniRule annotation1
Binding sitei248NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 140NADPUniRule annotation5
Nucleotide bindingi160 – 165NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:Arad_0002
OrganismiAgrobacterium radiobacter (strain K84 / ATCC BAA-868)
Taxonomic identifieri311403 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000001600 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001248681 – 286Shikimate dehydrogenase (NADP(+))Add BLAST286

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi311403.Arad_0002

Structurei

3D structure databases

ProteinModelPortaliB9JG24
SMRiB9JG24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 24Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiSPLIHGY
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

B9JG24-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDSRETHGL NAFVTGYPVK HSRSPLIHGY WLRTLNLAGS YRAVEVTPDD
60 70 80 90 100
FPAFIAALKD RSSGFVGGNV TIPHKEIAFK LADRPDELSE ELGASNTLWL
110 120 130 140 150
EDGLLHATNT DGRGFTANLD ECHPGWDRTD RAVILGAGGA SRAVIQAVRD
160 170 180 190 200
RGVNEIHVVN RTVERAQELS DRFGAQVHAH PMAALGEVMR SAGLFVNTTS
210 220 230 240 250
LGMENEVAPT IDFSPLAENA VVTDIVYVPL KTPLLAQAEE QGFATVDGLG
260 270 280
MLLHQAAPGF ETWFGKRPVV DEVLRALIIA DMDKHR
Length:286
Mass (Da):31,070
Last modified:March 24, 2009 - v1
Checksum:i1E88F6F37E69EE77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000628 Genomic DNA Translation: ACM24807.1
RefSeqiWP_007698676.1, NC_011985.1

Genome annotation databases

EnsemblBacteriaiACM24807; ACM24807; Arad_0002
KEGGiara:Arad_0002

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000628 Genomic DNA Translation: ACM24807.1
RefSeqiWP_007698676.1, NC_011985.1

3D structure databases

ProteinModelPortaliB9JG24
SMRiB9JG24
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi311403.Arad_0002

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM24807; ACM24807; Arad_0002
KEGGiara:Arad_0002

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiSPLIHGY
OrthoDBiPOG091H02DY

Enzyme and pathway databases

UniPathwayi
UPA00053;UER00087

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_AGRRK
AccessioniPrimary (citable) accession number: B9JG24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 7, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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