UniProtKB - B9J738 (TDH_AGRRK)
Protein
L-threonine 3-dehydrogenase
Gene
tdh
Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Functioni
Catalyzes the NAD+-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.UniRule annotation
Catalytic activityi
- EC:1.1.1.103UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation
: L-threonine degradation via oxydo-reductase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycine from L-threonine.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- L-threonine 3-dehydrogenase (tdh)
- 2-amino-3-ketobutyrate coenzyme A ligase (kbl)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 42 | Zinc 1; catalyticUniRule annotation | 1 | |
Active sitei | 44 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 47 | Charge relay systemUniRule annotation | 1 | |
Metal bindingi | 67 | Zinc 1; via tele nitrogen; catalyticUniRule annotation | 1 | |
Metal bindingi | 68 | Zinc 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 97 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 100 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 103 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 111 | Zinc 2UniRule annotation | 1 | |
Sitei | 152 | Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atomUniRule annotation | 1 | |
Binding sitei | 179 | NAD; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 199 | NADUniRule annotation | 1 | |
Binding sitei | 204 | NADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 266 – 268 | NADUniRule annotation | 3 | |
Nucleotide bindingi | 290 – 291 | NADUniRule annotation | 2 |
GO - Molecular functioni
- L-threonine 3-dehydrogenase activity Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- L-threonine catabolic process to glycine Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00046;UER00505 |
Names & Taxonomyi
Protein namesi | Recommended name: L-threonine 3-dehydrogenaseUniRule annotation (EC:1.1.1.103UniRule annotation)Short name: TDHUniRule annotation |
Gene namesi | Name:tdhUniRule annotation Ordered Locus Names:Arad_3118 |
Organismi | Agrobacterium radiobacter (strain K84 / ATCC BAA-868) |
Taxonomic identifieri | 311403 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000147257 | 1 – 345 | L-threonine 3-dehydrogenaseAdd BLAST | 345 |
Proteomic databases
PRIDEi | B9J738 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotationProtein-protein interaction databases
STRINGi | 311403.Arad_3118 |
Family & Domainsi
Sequence similaritiesi
Belongs to the zinc-containing alcohol dehydrogenase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105CPQ Bacteria COG1063 LUCA |
HOGENOMi | HOG000294686 |
KOi | K00060 |
OMAi | QIMGHEV |
OrthoDBi | 972769at2 |
Family and domain databases
HAMAPi | MF_00627 Thr_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR013149 ADH_C IPR013154 ADH_N IPR002328 ADH_Zn_CS IPR011032 GroES-like_sf IPR004627 L-Threonine_3-DHase IPR036291 NAD(P)-bd_dom_sf IPR020843 PKS_ER |
Pfami | View protein in Pfam PF08240 ADH_N, 1 hit PF00107 ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829 PKS_ER, 1 hit |
SUPFAMi | SSF50129 SSF50129, 1 hit SSF51735 SSF51735, 1 hit |
TIGRFAMsi | TIGR00692 tdh, 1 hit |
PROSITEi | View protein in PROSITE PS00059 ADH_ZINC, 1 hit |
i Sequence
Sequence statusi: Complete.
B9J738-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNMMKALVK SKPEVGLWME HVPVPEVGPN DVLIRVRKSA ICGTDVHIWN
60 70 80 90 100
WDQWAQKTIP VPMVVGHEFC GEIAEIGSAV TKYHVGERVS GEGHIVCGKC
110 120 130 140 150
RNCRAGRGHL CRNTLGVGVN RPGSFGEFVC IPESNVVPIP DDIPDEVAAI
160 170 180 190 200
FDPFGNAVHT ALSFDLVGED VLVTGAGPIG IMGAMVAKRS GARKVVITDI
210 220 230 240 250
NPNRLALAHK LGIDHVVDAS KENLADVMKS IGMTEGFDVG LEMSGAAPAF
260 270 280 290 300
RDMIDKMNNG GKIAILGIAP AGFEIDWNKV IFKMLNLKGI YGREMFETWY
310 320 330 340
KMIAFVQGGL DVSPVITHRI GIDDFREGFE AMRSGNSGKV VMDWF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000628 Genomic DNA Translation: ACM27145.1 |
RefSeqi | WP_012651899.1, NC_011985.1 |
Genome annotation databases
EnsemblBacteriai | ACM27145; ACM27145; Arad_3118 |
GeneIDi | 31556031 |
KEGGi | ara:Arad_3118 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000628 Genomic DNA Translation: ACM27145.1 |
RefSeqi | WP_012651899.1, NC_011985.1 |
3D structure databases
SMRi | B9J738 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 311403.Arad_3118 |
Proteomic databases
PRIDEi | B9J738 |
Genome annotation databases
EnsemblBacteriai | ACM27145; ACM27145; Arad_3118 |
GeneIDi | 31556031 |
KEGGi | ara:Arad_3118 |
Phylogenomic databases
eggNOGi | ENOG4105CPQ Bacteria COG1063 LUCA |
HOGENOMi | HOG000294686 |
KOi | K00060 |
OMAi | QIMGHEV |
OrthoDBi | 972769at2 |
Enzyme and pathway databases
UniPathwayi | UPA00046;UER00505 |
Family and domain databases
HAMAPi | MF_00627 Thr_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR013149 ADH_C IPR013154 ADH_N IPR002328 ADH_Zn_CS IPR011032 GroES-like_sf IPR004627 L-Threonine_3-DHase IPR036291 NAD(P)-bd_dom_sf IPR020843 PKS_ER |
Pfami | View protein in Pfam PF08240 ADH_N, 1 hit PF00107 ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829 PKS_ER, 1 hit |
SUPFAMi | SSF50129 SSF50129, 1 hit SSF51735 SSF51735, 1 hit |
TIGRFAMsi | TIGR00692 tdh, 1 hit |
PROSITEi | View protein in PROSITE PS00059 ADH_ZINC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDH_AGRRK | |
Accessioni | B9J738Primary (citable) accession number: B9J738 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | March 24, 2009 | |
Last modified: | September 18, 2019 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways