UniProtKB - B9EJA2 (CTTB2_MOUSE)
Cortactin-binding protein 2
Cttnbp2
Functioni
Regulates the dendritic spine distribution of CTTN/cortactin in hippocampal neurons, and thus controls dendritic spinogenesis and dendritic spine maintenance.
1 PublicationGO - Molecular functioni
- SH3 domain binding Source: MGI
GO - Biological processi
- brain development Source: Ensembl
- regulation of modification of postsynaptic actin cytoskeleton Source: MGI
- regulation of synapse organization Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Cortactin-binding protein 2Short name: CortBP2 |
Gene namesi | Name:Cttnbp2 Synonyms:Kiaa1758 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1353467, Cttnbp2 |
VEuPathDBi | HostDB:ENSMUSG00000000416 |
Subcellular locationi
Other locations
Note: Remains associated with dendritic spines even after glutamate stimulation.By similarity
Cytoskeleton
- postsynaptic actin cytoskeleton Source: SynGO
Other locations
- cell cortex Source: UniProtKB-SubCell
- dendritic spine Source: UniProtKB-SubCell
- glutamatergic synapse Source: SynGO
- synaptic vesicle Source: MGI
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 540 | P → A: Almost complete loss of CTTN-binding and loss of regulation of spine density; when associated with A-543. 1 Publication | 1 | |
Mutagenesisi | 543 | P → A: Almost complete loss of CTTN-binding and loss of regulation of spine density; when associated with A-540. 1 Publication | 1 | |
Mutagenesisi | 599 | P → A: Reduced CTTN-binding; when associated with A-602. 1 Publication | 1 | |
Mutagenesisi | 602 | P → A: Reduced CTTN-binding; when associated with A-599. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422172 | 1 – 1648 | Cortactin-binding protein 2Add BLAST | 1648 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 495 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 1510 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
PaxDbi | B9EJA2 |
PeptideAtlasi | B9EJA2 |
PRIDEi | B9EJA2 |
ProteomicsDBi | 279291 [B9EJA2-1] 279292 [B9EJA2-2] 279293 [B9EJA2-3] 279294 [B9EJA2-4] 279295 [B9EJA2-5] |
PTM databases
iPTMneti | B9EJA2 |
PhosphoSitePlusi | B9EJA2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000000416, Expressed in Ammon's horn and 233 other tissues |
ExpressionAtlasi | B9EJA2, baseline and differential |
Genevisiblei | B9EJA2, MM |
Interactioni
Subunit structurei
Interacts with CTTN/cortactin SH3 domain.
Interacts with STRN, STRN4/zinedin and MOB4/phocein; this interaction may regulate dendritic spine distribution of STRN and STRN4 in hippocampal neurons (By similarity). Activation of glutamate receptors weakens the interaction with STRN and STRN4 (By similarity).
By similarityGO - Molecular functioni
- SH3 domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 205963, 27 interactors |
IntActi | B9EJA2, 4 interactors |
STRINGi | 10090.ENSMUSP00000088089 |
Miscellaneous databases
RNActi | B9EJA2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 699 – 729 | ANK 1Add BLAST | 31 | |
Repeati | 733 – 762 | ANK 2Add BLAST | 30 | |
Repeati | 766 – 795 | ANK 3Add BLAST | 30 | |
Repeati | 799 – 828 | ANK 4Add BLAST | 30 | |
Repeati | 832 – 861 | ANK 5Add BLAST | 30 | |
Repeati | 901 – 931 | ANK 6Add BLAST | 31 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 26 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 200 – 250 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 322 – 439 | DisorderedSequence analysisAdd BLAST | 118 | |
Regioni | 451 – 476 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 492 – 608 | DisorderedSequence analysisAdd BLAST | 117 | |
Regioni | 1438 – 1492 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 1522 – 1648 | DisorderedSequence analysisAdd BLAST | 127 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 119 – 274 | Sequence analysisAdd BLAST | 156 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 200 – 216 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 229 – 250 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 331 – 345 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 360 – 433 | Polar residuesSequence analysisAdd BLAST | 74 | |
Compositional biasi | 452 – 476 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 536 – 554 | Pro residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 576 – 597 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 1474 – 1492 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 1532 – 1547 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 1548 – 1592 | Polar residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 1603 – 1631 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 1632 – 1648 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Keywords - Domaini
ANK repeat, Coiled coil, RepeatPhylogenomic databases
eggNOGi | ENOG502QWG2, Eukaryota |
GeneTreei | ENSGT00940000158293 |
HOGENOMi | CLU_028813_1_0_1 |
InParanoidi | B9EJA2 |
OMAi | MCPVEAL |
OrthoDBi | 264951at2759 |
PhylomeDBi | B9EJA2 |
TreeFami | TF325130 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR019131, Cortactin-binding_p2_N |
Pfami | View protein in Pfam PF12796, Ank_2, 2 hits PF09727, CortBP2, 2 hits |
SMARTi | View protein in SMART SM00248, ANK, 6 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 4 hits |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATDSASCEP DLSRTPGDTE GATAEAAKKE FDVDTLSKSE LRMLLSVMEG
60 70 80 90 100
ELEARDLVIE ALRARRKEVF IQERYGRFNL NDPFLALQRD YEAGPGDKEK
110 120 130 140 150
PVCTNPLSIL EAVMAHCRKM QERMSAQLVA AESRQKKLEM EKLQLQALEQ
160 170 180 190 200
EHKKLAAHLE EERGKNKHVV LMLVKECKQL SGKVVEEAQK LEEVMAQLEE
210 220 230 240 250
EKKKTSELEE QLSAEKQRSS GMEAQLEKQL SEFDTEREQL RAKLSREEAH
260 270 280 290 300
TTDLKEEIDK MKKMMEQMKK GSDGKPGLSL PRKTKDKRLA SISVATEGPV
310 320 330 340 350
TRSVACQTDV VTESTDPVKK LPLTVPIKPS TGSPLVPTNT KGNVGPSALL
360 370 380 390 400
IRPGIDRQSS HSDLGPSPPT ALPSSANRIE ENGPSTGNAP DLSNSTPSTP
410 420 430 440 450
SSTAPAAAQT PGTAPQNHSQ APTVHSLHSP CANTHPGLNP RIQAARFRFQ
460 470 480 490 500
GNANDPDQNG NNTQSPPSRD VSPTSRDNLV AKQLARNTVT QALSRFTSPQ
510 520 530 540 550
AGASSRLGVS PGGDAGTCPP VGRTGLKTPG AARVDRGNPP PIPPKKPGLS
560 570 580 590 600
QTPSPPHPQL RASNAGAKVD NKIVASPPST LPQGTKVVNE ENVPKSSSPQ
610 620 630 640 650
LPPKPSIDLT VAPAGCPVSA LATSQVGAWP AGTPGLNQPA CSDSSLVIPA
660 670 680 690 700
TVAFCSSINP VSASSRSPGA SDSLLVAASG WSPSLTPLLM SGGPAPLAGR
710 720 730 740 750
PTLLQQAAAQ GNVTLLSMLL NEEGLDINYS CEDGHSALYS AAKNGHTDCV
760 770 780 790 800
RLLLNAEARV DAADKNGFTP LCVAAAQGHF ECIELLTAYN ANINHSAAGG
810 820 830 840 850
QTPLYLACKN GNKECIKLLL EAGTDRSIKT RDGWTPIHAA VDTGNVDSLK
860 870 880 890 900
LLMYHRVRAH GNSLSSEEPK SGLFSLNGGE SPTGPSKPVV PADLINHADK
910 920 930 940 950
EGWTAAHIAA SKGFKNCLEV LCRHGGLEPE RRDKCNRTVH DVATDDCKHL
960 970 980 990 1000
LENLNALKIP LRISVGEIQP SNDVSDDFEC EHTICTLNIR KQTSWEDFSK
1010 1020 1030 1040 1050
AVSQALTNHF QAISSDGWWS LEDGTFNNAT DSCIGLGTSS IRSIMLGSMP
1060 1070 1080 1090 1100
WSTGQSFSQS PWDFLKKKKV EQVLALLSGP QEGCLSSVTY ASMIPLQMLQ
1110 1120 1130 1140 1150
NYLRLVEQYH NVIFHGPEGS LQDYIANQLA LCMKYRQMAA GFPCEIVRAE
1160 1170 1180 1190 1200
VDSGFSKEQL VDVFIRNACL IPVKQFPVKK KIIVILENLE KSSLSELLGD
1210 1220 1230 1240 1250
FLAPLENRST ESPCTFQKGN GTSECYYFHE NCFLVGTIAK ACLQGSDLLV
1260 1270 1280 1290 1300
QQHFRWVQLR WDCEPIQGLL QRFLRRKVVS KFRGQLPAPC DPVCKIVDWA
1310 1320 1330 1340 1350
LSVWRQLNSC LARLGTPEAL LGPKYFLSCP VVPGHAQATV KWMSKLWNAV
1360 1370 1380 1390 1400
IAPRVQEAIL SRASMNKQPG TGQTASKKYP SQGQQAVVRA ALSILLNKAV
1410 1420 1430 1440 1450
LHGCPLPRAE LDQQIADFKG GSFPLSIVSS YSKKKVESGA WRKVNTSPRK
1460 1470 1480 1490 1500
KPGHFSSPTW NKPDPKREGM RNKTIPHLNT NRNSSLSKQQ SLENDLSVTL
1510 1520 1530 1540 1550
TLDHRLSLGS DDEADLVKEL QSMCSSKSES DISKIADSRD DLRKFDSSRT
1560 1570 1580 1590 1600
NPGTSAPLNL RTPVPQKEAS PPSSRQTAEC SNSKSKTEMG VSSVKSFLPV
1610 1620 1630 1640
PRSKVAQCSQ NTKRNSSSSN TRQLEINNNS KEENWTLDKH EQVEKPNK
The sequence of this isoform differs from the canonical sequence as follows:
626-630: VGAWP → AGHPP
631-1648: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
681-710: WSPSLTPLLMSGGPAPLAGRPTLLQQAAAQ → KGIEVFYPFVKNVPYSLTSLNFFYVVLKRF
711-1648: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6Q1W8 | F6Q1W8_MOUSE | Cortactin-binding protein 2 | Cttnbp2 | 1,139 | Annotation score: | ||
D3Z1E3 | D3Z1E3_MOUSE | Cortactin-binding protein 2 | Cttnbp2 | 160 | Annotation score: | ||
D3Z551 | D3Z551_MOUSE | Cortactin-binding protein 2 | Cttnbp2 | 94 | Annotation score: | ||
F6XSM7 | F6XSM7_MOUSE | Cortactin-binding protein 2 | Cttnbp2 | 193 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 521 | V → F in AK028980 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 661 | V → F in AK028980 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1619 | S → SSS in AAI41408 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046473 | 626 – 680 | VGAWP…VAASG → KIQKVKCTREEPSC in isoform 5. 1 PublicationAdd BLAST | 55 | |
Alternative sequenceiVSP_046474 | 626 – 630 | VGAWP → AGHPP in isoform 2. 1 Publication | 5 | |
Alternative sequenceiVSP_046475 | 631 – 1648 | Missing in isoform 2. 1 PublicationAdd BLAST | 1018 | |
Alternative sequenceiVSP_046476 | 681 – 710 | WSPSL…QAAAQ → KGIEVFYPFVKNVPYSLTSL NFFYVVLKRF in isoform 3. 1 PublicationAdd BLAST | 30 | |
Alternative sequenceiVSP_046477 | 711 – 1648 | Missing in isoform 3. 1 PublicationAdd BLAST | 938 | |
Alternative sequenceiVSP_046478 | 1568 – 1648 | EASPP…EKPNK → KQSRSVFPEHQKKQQQQQYK ATRNQQQLQRRELDLRQTRT SRKTKQIGLPTPPTTT in isoform 4. 1 PublicationAdd BLAST | 81 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028980 mRNA No translation available. AK032356 mRNA Translation: BAC27832.1 AK078315 mRNA Translation: BAC37216.1 AC027654 Genomic DNA No translation available. AC158663 Genomic DNA No translation available. CH466533 Genomic DNA Translation: EDL13863.1 BC068156 mRNA No translation available. BQ769661 mRNA No translation available. BC141407 mRNA Translation: AAI41408.1 CB526439 mRNA No translation available. AK173254 mRNA Translation: BAD32532.1 Sequence problems. |
CCDSi | CCDS39432.1 [B9EJA2-1] |
RefSeqi | NP_525024.1, NM_080285.1 [B9EJA2-1] XP_006505167.1, XM_006505104.2 [B9EJA2-5] |
Genome annotation databases
Ensembli | ENSMUST00000090601; ENSMUSP00000088089; ENSMUSG00000000416 [B9EJA2-1] ENSMUST00000148602; ENSMUSP00000118432; ENSMUSG00000000416 [B9EJA2-2] |
GeneIDi | 30785 |
KEGGi | mmu:30785 |
UCSCi | uc009bak.1, mouse [B9EJA2-1] uc009ban.1, mouse [B9EJA2-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK028980 mRNA No translation available. AK032356 mRNA Translation: BAC27832.1 AK078315 mRNA Translation: BAC37216.1 AC027654 Genomic DNA No translation available. AC158663 Genomic DNA No translation available. CH466533 Genomic DNA Translation: EDL13863.1 BC068156 mRNA No translation available. BQ769661 mRNA No translation available. BC141407 mRNA Translation: AAI41408.1 CB526439 mRNA No translation available. AK173254 mRNA Translation: BAD32532.1 Sequence problems. |
CCDSi | CCDS39432.1 [B9EJA2-1] |
RefSeqi | NP_525024.1, NM_080285.1 [B9EJA2-1] XP_006505167.1, XM_006505104.2 [B9EJA2-5] |
3D structure databases
AlphaFoldDBi | B9EJA2 |
SMRi | B9EJA2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205963, 27 interactors |
IntActi | B9EJA2, 4 interactors |
STRINGi | 10090.ENSMUSP00000088089 |
PTM databases
iPTMneti | B9EJA2 |
PhosphoSitePlusi | B9EJA2 |
Proteomic databases
PaxDbi | B9EJA2 |
PeptideAtlasi | B9EJA2 |
PRIDEi | B9EJA2 |
ProteomicsDBi | 279291 [B9EJA2-1] 279292 [B9EJA2-2] 279293 [B9EJA2-3] 279294 [B9EJA2-4] 279295 [B9EJA2-5] |
Protocols and materials databases
Antibodypediai | 3970, 93 antibodies from 19 providers |
Genome annotation databases
Ensembli | ENSMUST00000090601; ENSMUSP00000088089; ENSMUSG00000000416 [B9EJA2-1] ENSMUST00000148602; ENSMUSP00000118432; ENSMUSG00000000416 [B9EJA2-2] |
GeneIDi | 30785 |
KEGGi | mmu:30785 |
UCSCi | uc009bak.1, mouse [B9EJA2-1] uc009ban.1, mouse [B9EJA2-3] |
Organism-specific databases
CTDi | 83992 |
MGIi | MGI:1353467, Cttnbp2 |
VEuPathDBi | HostDB:ENSMUSG00000000416 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QWG2, Eukaryota |
GeneTreei | ENSGT00940000158293 |
HOGENOMi | CLU_028813_1_0_1 |
InParanoidi | B9EJA2 |
OMAi | MCPVEAL |
OrthoDBi | 264951at2759 |
PhylomeDBi | B9EJA2 |
TreeFami | TF325130 |
Miscellaneous databases
BioGRID-ORCSi | 30785, 1 hit in 72 CRISPR screens |
ChiTaRSi | Cttnbp2, mouse |
PROi | PR:B9EJA2 |
RNActi | B9EJA2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000416, Expressed in Ammon's horn and 233 other tissues |
ExpressionAtlasi | B9EJA2, baseline and differential |
Genevisiblei | B9EJA2, MM |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR019131, Cortactin-binding_p2_N |
Pfami | View protein in Pfam PF12796, Ank_2, 2 hits PF09727, CortBP2, 2 hits |
SMARTi | View protein in SMART SM00248, ANK, 6 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CTTB2_MOUSE | |
Accessioni | B9EJA2Primary (citable) accession number: B9EJA2 Secondary accession number(s): G3X9L7 Q8C044 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2013 |
Last sequence update: | May 1, 2013 | |
Last modified: | May 25, 2022 | |
This is version 95 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot