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Protein

Oxysterol-binding protein-related protein 8

Gene

Osbpl8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner. Binds oxysterol, 25-hydroxycholesterol and cholesterol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei485PhosphatidylserineBy similarity1
Binding sitei540PhosphatidylserineBy similarity1
Binding sitei706Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei710Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei714Phosphatidylinositol 4-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processLipid transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482801 Acyl chain remodelling of PS

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein-related protein 8
Short name:
ORP-8
Short name:
OSBP-related protein 8
Gene namesi
Name:Osbpl8Imported
Synonyms:Kiaa14511 Publication, Orp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2443807 Osbpl8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei871 – 888HelicalSequence analysisAdd BLAST18

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are apparently healthy and show no developmental defects nor gross abnormality of glucose or lipid metabolism. However, they display a significant elevation of plasma HDL cholesterol and phospholipid levels. They also show a modest increase of APOA1, plus a number of mild gender- or diet-specific lipid metabolism phenotypes (PubMed:23554939). Deletion in bone marrow-derived cells, including macrophages, reduces atherosclerotic lesion progression (PubMed:25347070).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004341211 – 889Oxysterol-binding protein-related protein 8Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei65PhosphoserineBy similarity1
Modified residuei68PhosphoserineBy similarity1
Modified residuei314PhosphoserineCombined sources1
Modified residuei328PhosphoserineBy similarity1
Modified residuei342PhosphoserineBy similarity1
Modified residuei807PhosphoserineCombined sources1
Modified residuei808PhosphoserineCombined sources1
Modified residuei810PhosphoserineCombined sources1
Modified residuei814PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiB9EJ86
PaxDbiB9EJ86
PeptideAtlasiB9EJ86
PRIDEiB9EJ86

PTM databases

iPTMnetiB9EJ86
PhosphoSitePlusiB9EJ86

Expressioni

Tissue specificityi

Widely expressed. Most abundant in liver, spleen, kidney, brain and adipose tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000020189 Expressed in 240 organ(s), highest expression level in dorsal striatum
ExpressionAtlasiB9EJ86 baseline and differential
GenevisibleiB9EJ86 MM

Interactioni

Subunit structurei

Interacts with SPAG5. Interacts with NUP62.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100911

Structurei

3D structure databases

ProteinModelPortaliB9EJ86
SMRiB9EJ86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 265PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni420 – 425Phosphatidylinositol 4-phosphate bindingBy similarity6
Regioni420 – 425Phosphatidylserine bindingBy similarity6
Regioni482 – 485Phosphatidylinositol 4-phosphate bindingBy similarity4
Regioni514 – 515Phosphatidylinositol 4-phosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the OSBP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2210 Eukaryota
ENOG410XRW6 LUCA
GeneTreeiENSGT00550000074515
HOGENOMiHOG000233870
HOVERGENiHBG053375
InParanoidiB9EJ86
KOiK22285
OrthoDBiEOG091G0A3R
PhylomeDBiB9EJ86
TreeFamiTF312807

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037239 OSBP_sf
IPR000648 Oxysterol-bd
IPR018494 Oxysterol-bd_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR10972 PTHR10972, 1 hit
PfamiView protein in Pfam
PF01237 Oxysterol_BP, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SUPFAMiSSF144000 SSF144000, 1 hit
PROSITEiView protein in PROSITE
PS01013 OSBP, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B9EJ86-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEAALADGEP DRSSLLGDSK DVLGPSTVVA NSDEPQHLTP GKMSQRQGRD
60 70 80 90 100
ANPTPTRDLP QPSLSPASLH SQGFERGKED ISQNKDDSSL SMSKSKSESK
110 120 130 140 150
LYNGSEKDSS TSSKLTKKES LKVQKKNYRE EKKRATKELL STITDPSVIV
160 170 180 190 200
MADWLKIRGT LKSWTKLWCV LKPGVLLIYK TQKNGQWVGT VLLNACEIIE
210 220 230 240 250
RPSKKDGFCF KLFHPLEQSI WAVKGPKGEA VGSITQPLPS SYLIIRATSE
260 270 280 290 300
SDGRCWMDAL ELALKCSSLL KRTMVREGKE HDLSISSDST HVTLYGLLRA
310 320 330 340 350
NNLHSGDNFQ LNDSEIERQH FKDQDLYSDK SDKENDPEHD ESDNEVLGKS
360 370 380 390 400
EESDTDTSER QDDSYIDPEP VEPLKETTYM EQSHEELGEA GEASQTETVS
410 420 430 440 450
EENKSLIWTL LKQVRPGMDL SRVVLPTFIL EPRSFLDKLS DYYYHADFLS
460 470 480 490 500
EAALEENPYF RLKKVVKWYL SGFYKKPKGL KKPYNPILGE TFRCLWIHPR
510 520 530 540 550
TNSKTFYIAE QVSHHPPISA FYVSNRKDGF CLSGSILAKS KFYGNSLSAI
560 570 580 590 600
LEGEARLTFL NRGEDYVMTM PYAHCKGILY GTMTLELGGT VNITCQKTGY
610 620 630 640 650
SAILEFKLKP FLGSSDYVNQ ISGKLKLGKE VLATLEGHWD SEVFINDKKT
660 670 680 690 700
DNSEIFWNPT PDIKQWRLIR HTVKFEEQDD FESEKLWQRV TKAINAKDQT
710 720 730 740 750
EATQEKYVLE EAQRQAARDR KTKTQEWVCK LFELDPLTGE WHYKFSDTRP
760 770 780 790 800
WDPLNDMIQF EKDGVIQTKV KHRTPMVSVP KMKHKPTRQQ KKVVKGYSSP
810 820 830 840 850
EPDIQDSSGS EAQSVKPSTR RKKGIDLGDI QSSIESIKQT QEEIKRNIMA
860 870 880
LRNHLLSSTP ATDYFLQQKD YFVIFLLILL QVIINFIFK
Length:889
Mass (Da):101,269
Last modified:March 24, 2009 - v1
Checksum:i01565BEBAD48553C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J150A0A0R4J150_MOUSE
Oxysterol-binding protein
Osbpl8
847Annotation score:

Sequence cautioni

The sequence EDL21722 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121871 Genomic DNA No translation available.
AC124399 Genomic DNA No translation available.
CH466539 Genomic DNA Translation: EDL21721.1
CH466539 Genomic DNA Translation: EDL21722.1 Sequence problems.
BC141383 mRNA Translation: AAI41384.1
AK173174 mRNA Translation: BAD32452.1
CCDSiCCDS36056.1
RefSeqiNP_001003717.1, NM_001003717.1
NP_780698.2, NM_175489.3
XP_006513702.1, XM_006513639.2
UniGeneiMm.220204

Genome annotation databases

EnsembliENSMUST00000105275; ENSMUSP00000100911; ENSMUSG00000020189
GeneIDi237542
KEGGimmu:237542
UCSCiuc007gzy.1 mouse
uc007gzz.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121871 Genomic DNA No translation available.
AC124399 Genomic DNA No translation available.
CH466539 Genomic DNA Translation: EDL21721.1
CH466539 Genomic DNA Translation: EDL21722.1 Sequence problems.
BC141383 mRNA Translation: AAI41384.1
AK173174 mRNA Translation: BAD32452.1
CCDSiCCDS36056.1
RefSeqiNP_001003717.1, NM_001003717.1
NP_780698.2, NM_175489.3
XP_006513702.1, XM_006513639.2
UniGeneiMm.220204

3D structure databases

ProteinModelPortaliB9EJ86
SMRiB9EJ86
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100911

PTM databases

iPTMnetiB9EJ86
PhosphoSitePlusiB9EJ86

Proteomic databases

MaxQBiB9EJ86
PaxDbiB9EJ86
PeptideAtlasiB9EJ86
PRIDEiB9EJ86

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105275; ENSMUSP00000100911; ENSMUSG00000020189
GeneIDi237542
KEGGimmu:237542
UCSCiuc007gzy.1 mouse
uc007gzz.1 mouse

Organism-specific databases

CTDi114882
MGIiMGI:2443807 Osbpl8
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2210 Eukaryota
ENOG410XRW6 LUCA
GeneTreeiENSGT00550000074515
HOGENOMiHOG000233870
HOVERGENiHBG053375
InParanoidiB9EJ86
KOiK22285
OrthoDBiEOG091G0A3R
PhylomeDBiB9EJ86
TreeFamiTF312807

Enzyme and pathway databases

ReactomeiR-MMU-1482801 Acyl chain remodelling of PS

Miscellaneous databases

ChiTaRSiOsbpl8 mouse
PROiPR:B9EJ86
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020189 Expressed in 240 organ(s), highest expression level in dorsal striatum
ExpressionAtlasiB9EJ86 baseline and differential
GenevisibleiB9EJ86 MM

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037239 OSBP_sf
IPR000648 Oxysterol-bd
IPR018494 Oxysterol-bd_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR10972 PTHR10972, 1 hit
PfamiView protein in Pfam
PF01237 Oxysterol_BP, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SUPFAMiSSF144000 SSF144000, 1 hit
PROSITEiView protein in PROSITE
PS01013 OSBP, 1 hit
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOSBL8_MOUSE
AccessioniPrimary (citable) accession number: B9EJ86
Secondary accession number(s): G3X9N6, Q69ZJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: March 24, 2009
Last modified: November 7, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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