Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription termination factor 1b, mitochondrial

Gene

Mterf1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Transcription termination activity appears to be polarized with highest specificity for transcripts initiated on the light strand.1 Publication

GO - Molecular functioni

  • double-stranded DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation, Transcription termination

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor 1b, mitochondrial
Alternative name(s):
Mitochondrial transcription termination factor 1bImported
Short name:
mTERF1b
Gene namesi
Name:Mterf1bImported
Synonyms:Gm9897Imported, Mterf, Mterf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:3704243 Mterf1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Double knockout of Mterf1a and Mterf1b results in viable animals with no gross phenotype, and normal oxidative phosphorylation capacity. Steady-state mitochondrial DNA levels are normal. There are subtle effects on levels of mitochondrial transcripts: transcripts initiated at the light strand promoter and also situated downstream of the MTERF binding site are increased, levels of 7S RNA are reduced, while levels of other mitochondrial transcripts appear normal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37MitochondrionSequence analysisAdd BLAST37
ChainiPRO_000043015238 – 379Transcription termination factor 1b, mitochondrialSequence analysisAdd BLAST342

Post-translational modificationi

Phosphoprotein with mostly four phosphate groups. While the DNA-binding activity is unaffected by the phosphorylation state, only the phosphorylated form of the protein is active for termination activity. Functioning seems to be regulated by phosphorylation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB9EJ57
PeptideAtlasiB9EJ57
PRIDEiB9EJ57

Expressioni

Tissue specificityi

Expressed strongly in the heart and at lower levels in brain, liver and kidney.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei144Interaction with DNABy similarity1
Sitei184Interaction with DNABy similarity1
Sitei332Interaction with DNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000135408

Structurei

3D structure databases

ProteinModelPortaliB9EJ57
SMRiB9EJ57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 152Interaction with DNABy similarity2
Regioni229 – 233Interaction with DNABy similarity5
Regioni306 – 313Interaction with DNABy similarity8
Regioni337 – 340Interaction with DNABy similarity4
Regioni366 – 373Interaction with DNABy similarity8

Domaini

Contains nine structural repeats of about 35 residues, where each repeat contains three helices. The repeats form a left-handed superhelical assembly with a solenoid structure that wraps itself around DNA (By similarity).By similarity

Sequence similaritiesi

Belongs to the mTERF family.Sequence analysis

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1267 Eukaryota
ENOG410XT49 LUCA
HOGENOMiHOG000252957
HOVERGENiHBG052524
KOiK15031
PhylomeDBiB9EJ57

Family and domain databases

Gene3Di1.25.70.10, 1 hit
InterProiView protein in InterPro
IPR003690 MTERF
IPR038538 MTERF_sf
PfamiView protein in Pfam
PF02536 mTERF, 1 hit
SMARTiView protein in SMART
SM00733 Mterf, 6 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B9EJ57-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASRNIWCVR RNFLFDLRGW MLQYSAEVFL KSISFRTFSV ECDSKDKESL
60 70 80 90 100
EEEREDLLSN LVTMGVDIDM ARRRQPGVFN KAVTNEQELK IFLLSKGASD
110 120 130 140 150
KVIGSIISRY PRAITRTPES LSKRWDLWRK IMASDLEIVN ILERSPESFF
160 170 180 190 200
RSNNNLNLEN NIKFLCSVGL THKCLCRLLT NAPRTFSNSL NLNKQMVEFL
210 220 230 240 250
QETGMSLGHN DPRDFVRKII SKNPSILIQS TKRVKTNIEF LQSTFNLNKQ
260 270 280 290 300
DLLLLICGPG ARILDLSNDC TKKNYTNIRE RLLSLGCSEE EVQRFVLSYL
310 320 330 340 350
NMVFLSEKKF NDKIDCLIEE KISASQIIEN PRILDSSINT LKTRIRELSH
360 370
AGYDLSTSSI ALLSWSQRRY EAKLKRLCG
Length:379
Mass (Da):43,761
Last modified:March 24, 2009 - v1
Checksum:iDA43F79AB4FE1F3D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A096P6K7A0A096P6K7_MOUSE
Transcription termination factor 1b...
Mterf1b
381Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068609 Genomic DNA No translation available.
CH466600 Genomic DNA Translation: EDL14630.1
CH466600 Genomic DNA Translation: EDL14631.1
BC141337 mRNA Translation: AAI41338.1
RefSeqiNP_001036135.2, NM_001042670.1
UniGeneiMm.129815

Genome annotation databases

GeneIDi208595
KEGGimmu:208595

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068609 Genomic DNA No translation available.
CH466600 Genomic DNA Translation: EDL14630.1
CH466600 Genomic DNA Translation: EDL14631.1
BC141337 mRNA Translation: AAI41338.1
RefSeqiNP_001036135.2, NM_001042670.1
UniGeneiMm.129815

3D structure databases

ProteinModelPortaliB9EJ57
SMRiB9EJ57
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000135408

Proteomic databases

PaxDbiB9EJ57
PeptideAtlasiB9EJ57
PRIDEiB9EJ57

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi208595
KEGGimmu:208595

Organism-specific databases

CTDi208595
MGIiMGI:3704243 Mterf1b

Phylogenomic databases

eggNOGiKOG1267 Eukaryota
ENOG410XT49 LUCA
HOGENOMiHOG000252957
HOVERGENiHBG052524
KOiK15031
PhylomeDBiB9EJ57

Miscellaneous databases

PROiPR:B9EJ57
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.70.10, 1 hit
InterProiView protein in InterPro
IPR003690 MTERF
IPR038538 MTERF_sf
PfamiView protein in Pfam
PF02536 mTERF, 1 hit
SMARTiView protein in SMART
SM00733 Mterf, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiMTF1B_MOUSE
AccessioniPrimary (citable) accession number: B9EJ57
Secondary accession number(s): L7N482
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: March 24, 2009
Last modified: November 7, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again