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Protein

Copper-transporting ATPase PAA2, chloroplastic

Gene

PAA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates copper transfer across the chloroplast thylakoid membrane. Required for copper delivery into the thylakoids lumen, which is essential for the function of copper proteins.1 Publication

Catalytic activityi

ATP + H2O + Cu2+(Side 1) = ADP + phosphate + Cu2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi87CopperPROSITE-ProRule annotation1
Metal bindingi90CopperPROSITE-ProRule annotation1
Active sitei5484-aspartylphosphate intermediateBy similarity1
Metal bindingi762MagnesiumPROSITE-ProRule annotation1
Metal bindingi766MagnesiumPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi761 – 768ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper-exporting ATPase activity Source: UniProtKB-EC
  • copper ion transmembrane transporter activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • copper ion transport Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processCopper transport, Ion transport, Transport
LigandATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G21930-MONOMER
MetaCyc:MONOMER-14496
BRENDAi3.6.3.4 399

Protein family/group databases

TCDBi3.A.3.5.12 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting ATPase PAA2, chloroplastic (EC:3.6.3.4)
Alternative name(s):
Protein HEAVY METAL ATPASE 8
Gene namesi
Name:PAA2
Synonyms:HMA8
Ordered Locus Names:At5g21930
ORF Names:F13M11, T6G21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G21930
TAIRilocus:504956435 AT5G21930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21
Transmembranei274 – 294HelicalSequence analysisAdd BLAST21
Transmembranei445 – 465HelicalSequence analysisAdd BLAST21
Transmembranei499 – 519HelicalSequence analysisAdd BLAST21
Transmembranei822 – 842HelicalSequence analysisAdd BLAST21
Transmembranei846 – 866HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

High-chlorophyll-fluorescence phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65ChloroplastSequence analysisAdd BLAST65
ChainiPRO_000041685866 – 883Copper-transporting ATPase PAA2, chloroplasticAdd BLAST818

Proteomic databases

PaxDbiB9DFX7
PRIDEiB9DFX7

Expressioni

Tissue specificityi

Expressed in the shoots only and not in the roots.1 Publication

Gene expression databases

ExpressionAtlasiB9DFX7 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G21930.1

Structurei

Secondary structure

1883
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi559 – 568Combined sources10
Helixi570 – 580Combined sources11
Turni581 – 583Combined sources3
Helixi587 – 598Combined sources12
Beta strandi609 – 612Combined sources4
Turni613 – 615Combined sources3
Beta strandi616 – 621Combined sources6
Beta strandi624 – 629Combined sources6
Helixi631 – 637Combined sources7
Helixi644 – 655Combined sources12
Beta strandi670 – 676Combined sources7
Turni677 – 679Combined sources3
Beta strandi680 – 688Combined sources9

3D structure databases

ProteinModelPortaliB9DFX7
SMRiB9DFX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 147HMAPROSITE-ProRule annotationAdd BLAST71

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207 Eukaryota
COG2217 LUCA
HOGENOMiHOG000250397
InParanoidiB9DFX7
KOiK01533
OMAiADSEHPV
OrthoDBiEOG0936082Z
PhylomeDBiB9DFX7

Family and domain databases

CDDicd00371 HMA, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 1 hit
SUPFAMiSSF55008 SSF55008, 1 hit
SSF56784 SSF56784, 3 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 2 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS50846 HMA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: B9DFX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNLLRFPL PPPSSLHIRP SKFLVNRCFP RLRRSRIRRH CSRPFFLVSN
60 70 80 90 100
SVEISTQSFE STESSIESVK SITSDTPILL DVSGMMCGGC VARVKSVLMS
110 120 130 140 150
DDRVASAVVN MLTETAAVKF KPEVEVTADT AESLAKRLTE SGFEAKRRVS
160 170 180 190 200
GMGVAENVKK WKEMVSKKED LLVKSRNRVA FAWTLVALCC GSHTSHILHS
210 220 230 240 250
LGIHIAHGGI WDLLHNSYVK GGLAVGALLG PGRELLFDGI KAFGKRSPNM
260 270 280 290 300
NSLVGLGSMA AFSISLISLV NPELEWDASF FDEPVMLLGF VLLGRSLEER
310 320 330 340 350
AKLQASTDMN ELLSLISTQS RLVITSSDNN TPVDSVLSSD SICINVSVDD
360 370 380 390 400
IRVGDSLLVL PGETFPVDGS VLAGRSVVDE SMLTGESLPV FKEEGCSVSA
410 420 430 440 450
GTINWDGPLR IKASSTGSNS TISKIVRMVE DAQGNAAPVQ RLADAIAGPF
460 470 480 490 500
VYTIMSLSAM TFAFWYYVGS HIFPDVLLND IAGPDGDALA LSLKLAVDVL
510 520 530 540 550
VVSCPCALGL ATPTAILIGT SLGAKRGYLI RGGDVLERLA SIDCVALDKT
560 570 580 590 600
GTLTEGRPVV SGVASLGYEE QEVLKMAAAV EKTATHPIAK AIVNEAESLN
610 620 630 640 650
LKTPETRGQL TEPGFGTLAE IDGRFVAVGS LEWVSDRFLK KNDSSDMVKL
660 670 680 690 700
ESLLDHKLSN TSSTSRYSKT VVYVGREGEG IIGAIAISDC LRQDAEFTVA
710 720 730 740 750
RLQEKGIKTV LLSGDREGAV ATVAKNVGIK SESTNYSLSP EKKFEFISNL
760 770 780 790 800
QSSGHRVAMV GDGINDAPSL AQADVGIALK IEAQENAASN AASVILVRNK
810 820 830 840 850
LSHVVDALSL AQATMSKVYQ NLAWAIAYNV ISIPIAAGVL LPQYDFAMTP
860 870 880
SLSGGLMALS SIFVVSNSLL LQLHKSETSK NSL
Length:883
Mass (Da):94,260
Last modified:March 24, 2009 - v1
Checksum:iF5E365AB6B7A90C3
GO

Sequence cautioni

The sequence AAO73891 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAC34486 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312L → P in AAP55720 (PubMed:15772282).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297817 mRNA Translation: AAP55720.1
AC140977 Genomic DNA Translation: AAO73891.1 Sequence problems.
AL589883 Genomic DNA Translation: CAC34486.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92956.1
CP002688 Genomic DNA Translation: AED92957.1
AK316941 mRNA Translation: BAH19644.1
RefSeqiNP_001031920.1, NM_001036843.1 [B9DFX7-1]
NP_680181.2, NM_147876.5 [B9DFX7-1]
UniGeneiAt.44341

Genome annotation databases

EnsemblPlantsiAT5G21930.1; AT5G21930.1; AT5G21930 [B9DFX7-1]
AT5G21930.2; AT5G21930.2; AT5G21930 [B9DFX7-1]
GeneIDi832253
GrameneiAT5G21930.1; AT5G21930.1; AT5G21930 [B9DFX7-1]
AT5G21930.2; AT5G21930.2; AT5G21930 [B9DFX7-1]
KEGGiath:AT5G21930

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHMA8_ARATH
AccessioniPrimary (citable) accession number: B9DFX7
Secondary accession number(s): Q7Y051, Q9C594
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 24, 2009
Last modified: April 25, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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