UniProtKB - B9DFX7 (HMA8_ARATH)
Copper-transporting ATPase PAA2, chloroplastic
PAA2
Functioni
Mediates copper transfer across the chloroplast thylakoid membrane. Required for copper delivery into the thylakoids lumen, which is essential for the function of copper proteins.
1 PublicationCatalytic activityi
- EC:7.2.2.9
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 87 | CopperPROSITE-ProRule annotation | 1 | |
Metal bindingi | 90 | CopperPROSITE-ProRule annotation | 1 | |
Active sitei | 548 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 762 | MagnesiumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 766 | MagnesiumPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 761 – 768 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- copper ion binding Source: GO_Central
- copper ion transmembrane transporter activity Source: TAIR
- P-type divalent copper transporter activity Source: GO_Central
GO - Biological processi
- copper ion homeostasis Source: GO_Central
- copper ion transport Source: TAIR
Keywordsi
Molecular function | Translocase |
Biological process | Copper transport, Ion transport, Transport |
Ligand | ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:MONOMER-14496 |
BRENDAi | 7.2.2.9, 399 |
Protein family/group databases
TCDBi | 3.A.3.5.12, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Copper-transporting ATPase PAA2, chloroplastic (EC:7.2.2.9)Alternative name(s): Protein HEAVY METAL ATPASE 8 |
Gene namesi | Name:PAA2 Synonyms:HMA8 Ordered Locus Names:At5g21930 ORF Names:F13M11, T6G21 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G21930 |
TAIRi | locus:504956435, AT5G21930 |
Subcellular locationi
Chloroplast
- chloroplast thylakoid membrane 1 Publication; Multi-pass membrane protein 1 Publication
Chloroplast
- chloroplast thylakoid membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 179 – 199 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 250 – 270 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 274 – 294 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 445 – 465 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 499 – 519 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 822 – 842 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 846 – 866 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, ThylakoidPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 65 | ChloroplastSequence analysisAdd BLAST | 65 | |
ChainiPRO_0000416858 | 66 – 883 | Copper-transporting ATPase PAA2, chloroplasticAdd BLAST | 818 |
Proteomic databases
PaxDbi | B9DFX7 |
ProteomicsDBi | 230256 [B9DFX7-1] |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | B9DFX7, baseline and differential |
Structurei
Secondary structure
3D structure databases
SMRi | B9DFX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 77 – 147 | HMAPROSITE-ProRule annotationAdd BLAST | 71 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0207, Eukaryota |
InParanoidi | B9DFX7 |
OMAi | MALEPMG |
OrthoDBi | 649559at2759 |
PhylomeDBi | B9DFX7 |
Family and domain databases
CDDi | cd00371, HMA, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006121, HMA_dom IPR036163, HMA_dom_sf IPR027256, P-typ_ATPase_IB IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00403, HMA, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF55008, SSF55008, 1 hit SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01525, ATPase-IB_hvy, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit PS50846, HMA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASNLLRFPL PPPSSLHIRP SKFLVNRCFP RLRRSRIRRH CSRPFFLVSN
60 70 80 90 100
SVEISTQSFE STESSIESVK SITSDTPILL DVSGMMCGGC VARVKSVLMS
110 120 130 140 150
DDRVASAVVN MLTETAAVKF KPEVEVTADT AESLAKRLTE SGFEAKRRVS
160 170 180 190 200
GMGVAENVKK WKEMVSKKED LLVKSRNRVA FAWTLVALCC GSHTSHILHS
210 220 230 240 250
LGIHIAHGGI WDLLHNSYVK GGLAVGALLG PGRELLFDGI KAFGKRSPNM
260 270 280 290 300
NSLVGLGSMA AFSISLISLV NPELEWDASF FDEPVMLLGF VLLGRSLEER
310 320 330 340 350
AKLQASTDMN ELLSLISTQS RLVITSSDNN TPVDSVLSSD SICINVSVDD
360 370 380 390 400
IRVGDSLLVL PGETFPVDGS VLAGRSVVDE SMLTGESLPV FKEEGCSVSA
410 420 430 440 450
GTINWDGPLR IKASSTGSNS TISKIVRMVE DAQGNAAPVQ RLADAIAGPF
460 470 480 490 500
VYTIMSLSAM TFAFWYYVGS HIFPDVLLND IAGPDGDALA LSLKLAVDVL
510 520 530 540 550
VVSCPCALGL ATPTAILIGT SLGAKRGYLI RGGDVLERLA SIDCVALDKT
560 570 580 590 600
GTLTEGRPVV SGVASLGYEE QEVLKMAAAV EKTATHPIAK AIVNEAESLN
610 620 630 640 650
LKTPETRGQL TEPGFGTLAE IDGRFVAVGS LEWVSDRFLK KNDSSDMVKL
660 670 680 690 700
ESLLDHKLSN TSSTSRYSKT VVYVGREGEG IIGAIAISDC LRQDAEFTVA
710 720 730 740 750
RLQEKGIKTV LLSGDREGAV ATVAKNVGIK SESTNYSLSP EKKFEFISNL
760 770 780 790 800
QSSGHRVAMV GDGINDAPSL AQADVGIALK IEAQENAASN AASVILVRNK
810 820 830 840 850
LSHVVDALSL AQATMSKVYQ NLAWAIAYNV ISIPIAAGVL LPQYDFAMTP
860 870 880
SLSGGLMALS SIFVVSNSLL LQLHKSETSK NSL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4K8C8 | F4K8C8_ARATH | P-type ATPase of Arabidopsis 2 | PAA2 ATHMA8, HEAVY METAL ATPASE 8, HMA8, P-type ATPase of Arabidopsis 2, At5g21930 | 860 | Annotation score: | ||
A0A1P8BE56 | A0A1P8BE56_ARATH | P-type ATPase of Arabidopsis 2 | PAA2 ATHMA8, HEAVY METAL ATPASE 8, HMA8, P-type ATPase of Arabidopsis 2, At5g21930 | 820 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 312 | L → P in AAP55720 (PubMed:15772282).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY297817 mRNA Translation: AAP55720.1 AC140977 Genomic DNA Translation: AAO73891.1 Sequence problems. AL589883 Genomic DNA Translation: CAC34486.1 Sequence problems. CP002688 Genomic DNA Translation: AED92956.1 CP002688 Genomic DNA Translation: AED92957.1 AK316941 mRNA Translation: BAH19644.1 |
RefSeqi | NP_001031920.1, NM_001036843.1 [B9DFX7-1] NP_680181.2, NM_147876.5 [B9DFX7-1] |
Genome annotation databases
EnsemblPlantsi | AT5G21930.1; AT5G21930.1; AT5G21930 [B9DFX7-1] AT5G21930.2; AT5G21930.2; AT5G21930 [B9DFX7-1] |
GeneIDi | 832253 |
Gramenei | AT5G21930.1; AT5G21930.1; AT5G21930 [B9DFX7-1] AT5G21930.2; AT5G21930.2; AT5G21930 [B9DFX7-1] |
KEGGi | ath:AT5G21930 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY297817 mRNA Translation: AAP55720.1 AC140977 Genomic DNA Translation: AAO73891.1 Sequence problems. AL589883 Genomic DNA Translation: CAC34486.1 Sequence problems. CP002688 Genomic DNA Translation: AED92956.1 CP002688 Genomic DNA Translation: AED92957.1 AK316941 mRNA Translation: BAH19644.1 |
RefSeqi | NP_001031920.1, NM_001036843.1 [B9DFX7-1] NP_680181.2, NM_147876.5 [B9DFX7-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5LBK | X-ray | 1.75 | A/B | 557-689 | [»] | |
SMRi | B9DFX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G21930.2 |
Protein family/group databases
TCDBi | 3.A.3.5.12, the p-type atpase (p-atpase) superfamily |
Proteomic databases
PaxDbi | B9DFX7 |
ProteomicsDBi | 230256 [B9DFX7-1] |
Genome annotation databases
EnsemblPlantsi | AT5G21930.1; AT5G21930.1; AT5G21930 [B9DFX7-1] AT5G21930.2; AT5G21930.2; AT5G21930 [B9DFX7-1] |
GeneIDi | 832253 |
Gramenei | AT5G21930.1; AT5G21930.1; AT5G21930 [B9DFX7-1] AT5G21930.2; AT5G21930.2; AT5G21930 [B9DFX7-1] |
KEGGi | ath:AT5G21930 |
Organism-specific databases
Araporti | AT5G21930 |
TAIRi | locus:504956435, AT5G21930 |
Phylogenomic databases
eggNOGi | KOG0207, Eukaryota |
InParanoidi | B9DFX7 |
OMAi | MALEPMG |
OrthoDBi | 649559at2759 |
PhylomeDBi | B9DFX7 |
Enzyme and pathway databases
BioCyci | ARA:MONOMER-14496 |
BRENDAi | 7.2.2.9, 399 |
Miscellaneous databases
PROi | PR:B9DFX7 |
Gene expression databases
ExpressionAtlasi | B9DFX7, baseline and differential |
Family and domain databases
CDDi | cd00371, HMA, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006121, HMA_dom IPR036163, HMA_dom_sf IPR027256, P-typ_ATPase_IB IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00403, HMA, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF55008, SSF55008, 1 hit SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01525, ATPase-IB_hvy, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit PS50846, HMA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HMA8_ARATH | |
Accessioni | B9DFX7Primary (citable) accession number: B9DFX7 Secondary accession number(s): Q7Y051, Q9C594 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2012 |
Last sequence update: | March 24, 2009 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families