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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei154Allosteric activator ADPUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei244Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:SPN23F08180
OrganismiStreptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)
Taxonomic identifieri561276 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001923821 – 335ATP-dependent 6-phosphofructokinaseAdd BLAST335

Proteomic databases

PRIDEiB8ZNU0

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8ZNU0
SMRiB8ZNU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 25Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni185 – 187Allosteric activator ADP bindingUniRule annotation3
Regioni213 – 215Allosteric activator ADP bindingUniRule annotation3
Regioni250 – 253Substrate bindingUniRule annotation4

Sequence similaritiesi

Phylogenomic databases

KOiK00850
OMAiGKLHSII

Family and domain databases

CDDicd00763 Bacterial_PFK, 1 hit
HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

B8ZNU0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRIAVLTSG GDAPGMNAAI RAVVRQAISE GMEVFGIYDG YAGMVAGEIH
60 70 80 90 100
PLDAASVGDI ISRGGTFLHS ARYPEFAQLE GQLKGIEQLK KHGIEGVVVI
110 120 130 140 150
GGDGSYHGAM RLTEHGFPAI GLPGTIDNDI VGTDFTIGFD TAVTTAMDAI
160 170 180 190 200
DKIRDTSSSH RRTFVIEVMG RNAGDIALWA GIATGADEII IPEAGFKMED
210 220 230 240 250
IVASIKAGYE CGKKHNIIVL AEGVMSAAEF GQKLKEAGDT SDLRVTELGH
260 270 280 290 300
IQRGGSPTAR DRVLASRMGA HAVKLLKEGI GGVAVGIRNE KMVENPILGT
310 320 330
AEEGALFSLT AEGKIVVNNP HKADIELSSL NKSLS
Length:335
Mass (Da):35,174
Last modified:March 3, 2009 - v1
Checksum:i2DFFBAACC58CAF66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211187 Genomic DNA Translation: CAR68652.1
RefSeqiWP_000820852.1, NC_011900.1

Genome annotation databases

EnsemblBacteriaiCAR68652; CAR68652; SPN23F08180
KEGGisne:SPN23F08180

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211187 Genomic DNA Translation: CAR68652.1
RefSeqiWP_000820852.1, NC_011900.1

3D structure databases

ProteinModelPortaliB8ZNU0
SMRiB8ZNU0
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB8ZNU0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR68652; CAR68652; SPN23F08180
KEGGisne:SPN23F08180

Phylogenomic databases

KOiK00850
OMAiGKLHSII

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

Family and domain databases

CDDicd00763 Bacterial_PFK, 1 hit
HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_STRPJ
AccessioniPrimary (citable) accession number: B8ZNU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 10, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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