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Protein

Beta-mannosidase B

Gene

mndB

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers mannobiose over mannotriose and has no activity against polymeric mannan. Is also severely restricted by galactosyl substitutions at the +1 subsite (By similarity).By similarity

Miscellaneous

In contrast to clade A beta-mannosidases, which are likely secreted, clade B proteins appear to be intracellular.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathwayi: N-glycan degradation

This protein is involved in the pathway N-glycan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei432Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00280

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase B (EC:3.2.1.25)
Alternative name(s):
Mannanase B
Short name:
Mannase B
Gene namesi
Name:mndB
ORF Names:AFLA_117830
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_117830

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003946531 – 844Beta-mannosidase BAdd BLAST844

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi723N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5059.CADAFLAP00012355

Structurei

3D structure databases

ProteinModelPortaliB8NW36
SMRiB8NW36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000186861
KOiK01192
OMAiMFANFDY
OrthoDBiEOG092C0CWO

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR036156 Beta-gal/glucu_dom_sf
IPR008979 Galactose-bd-like_sf
IPR006102 Glyco_hydro_2_Ig-like
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00703 Glyco_hydro_2, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

B8NW36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFSQYPLS TGWSFKDSDD QSPEAWMPVP VVPSVAHQDL QANQKLKNPY
60 70 80 90 100
IGFNELDARW VNDKSWTYRT VFQKPAVAAG SSIILAFDGL DTFATVKLDG
110 120 130 140 150
SVILQSDNMF LAHRVDVTKA LEAEGDHVLE IDFDCAMRRA RELREKDTKH
160 170 180 190 200
NWASFNGDPA RMAVRKAQYH WGWDWGPLLS TAGIWREVRL EVYSAKISDL
210 220 230 240 250
WTEVELASDH QTARVSAFTE VDAADSVDSY KASFLLSLHG KEVAREVATL
260 270 280 290 300
KDKVAKVTFD VTQPSLWWPN GYGDPALYEI SVSLEKEDCE IHSVSKKIGI
310 320 330 340 350
RTAELIQQPD RHGKSFFFRI NGVDVFCGGS CWIPADNLLP SITAERYRKW
360 370 380 390 400
IELMVAGRQV MIRVWGGGCY EDDSFYQACD ELGVLVWQDF MFGCGNYPTW
410 420 430 440 450
PELLESIEKE ANYNVRRLRH HPSIVVYVGN NEDYQVQESA GLVYDYEDKN
460 470 480 490 500
PENWLKTDFP ARYIYEKLLP SVVEKLSPKT VYHPGSPWGD GKITSDPTVG
510 520 530 540 550
DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI EYFVENEADK
560 570 580 590 600
YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETYVYL TQVVQAETMM
610 620 630 640 650
FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAFYAVA
660 670 680 690 700
RVLKPIAVGV RREHHDWSVT HAQPPKTSKY ELWIASSLQK ETVGTIELRF
710 720 730 740 750
LSVNTGLDVR APILRDNVKI VPNGTTNILE GVINHKAQPE PHVLAARLWV
760 770 780 790 800
DGEVTARDVD WPQPFKYLDL SDRGLEVNKV SESGNEQKLL ITAKKPVKCL
810 820 830 840
VFEERDGIRV SDSAMDIVPG DGQTVTVTGL KAGDAPLKYK YLGQ
Length:844
Mass (Da):96,003
Last modified:March 3, 2009 - v1
Checksum:iCB0DFBDDBC8C0ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963485 Genomic DNA Translation: EED45554.1
RefSeqiXP_002384490.1, XM_002384449.1

Genome annotation databases

EnsemblFungiiEED45554; EED45554; AFLA_117830
GeneIDi7921506
KEGGiafv:AFLA_117830

Similar proteinsi

Entry informationi

Entry nameiMANBB_ASPFN
AccessioniPrimary (citable) accession number: B8NW36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 3, 2009
Last modified: February 28, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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