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Protein

Thiamine thiazole synthase

Gene

AFLA_037160

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Substrate; via amide nitrogenUniRule annotation1
Binding sitei115Substrate; via amide nitrogenUniRule annotation1
Binding sitei182Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei218SubstrateUniRule annotation1
Binding sitei233SubstrateUniRule annotation1
Binding sitei285Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
ORF Names:AFLA_037160Imported
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)Imported
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_037160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2162,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-216 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi5059.CADAFLAP00003090

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 108Substrate bindingUniRule annotation2
Regioni295 – 297Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

B8N4L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPAAIYEP TVAATGLKGK VVVPETVPVE GASQTKLLDH FGGKWDEFKF
60 70 80 90 100
APIRESQVSR AMTRRYFEDL DKYAESDVVI VGAGSCGLST AYVLAKARPD
110 120 130 140 150
LKIAIVEASV SPGGGAWLGG QLFSAMVMRR PAEVFLNELG VPYEEDANPN
160 170 180 190 200
YVVVKHASLF TSTLMSKVLS FPNVKLFNAT AVEDLITRPT ENGNPQIAGV
210 220 230 240 250
VVNWTLVTLH HDDHSCMDPN TINAPVIIST TGHDGPFGAF CAKRLVSMGS
260 270 280 290 300
VDKLGGMRGL DMNSAEDAIV KNTREVTKGL IIGGMELSEI DGFNRMGPTF
310 320
GAMVLSGVKA AEEALKVFDE RQRECAE
Length:327
Mass (Da):35,109
Last modified:March 3, 2009 - v1
Checksum:iE5EBA8A3FF2F053D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963473 Genomic DNA Translation: EED56443.1
RefSeqiXP_002375225.1, XM_002375184.1

Genome annotation databases

EnsemblFungiiEED56443; EED56443; AFLA_037160
GeneIDi7913863
KEGGiafv:AFLA_037160

Similar proteinsi

Entry informationi

Entry nameiB8N4L2_ASPFN
AccessioniPrimary (citable) accession number: B8N4L2
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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