UniProtKB - B8GZM2 (PLED_CAUVN)
Protein
Response regulator PleD
Gene
pleD
Organism
Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus)
Status
Functioni
Response regulator that is part of a signal transduction pathway controlling cell differentiation in the swarmer-to-stalked cell transition.1 Publication
Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger.1 Publication
Catalytic activityi
- EC:2.7.7.651 Publication
Cofactori
Mg2+1 PublicationNote: Binds 2 Mg2+ per monomer.1 Publication
Activity regulationi
Allosterically inhibited by dimers of the product c-di-GMP.1 Publication
: 3',5'-cyclic di-GMP biosynthesis Pathwayi
This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 9 | Magnesium 11 Publication | 1 | |
Metal bindingi | 10 | Magnesium 13 Publications | 1 | |
Metal bindingi | 53 | Magnesium 13 Publications | 1 | |
Metal bindingi | 55 | Magnesium 1; via carbonyl oxygen3 Publications | 1 | |
Sitei | 178 | Allosteric product binding, non-activate state | 1 | |
Sitei | 313 | Allosteric product binding, active state | 1 | |
Metal bindingi | 327 | Magnesium 21 Publication | 1 | |
Metal bindingi | 328 | Magnesium 2; via carbonyl oxygen1 Publication | 1 | |
Sitei | 332 | Transition state stabilizerSequence analysis | 1 | |
Binding sitei | 335 | Substrate2 Publications | 1 | |
Binding sitei | 344 | Substrate2 Publications | 1 | |
Sitei | 359 | Allosteric product phosphate group binding, activate and inactive state | 1 | |
Sitei | 362 | Allosteric product binding, activate and inactive state | 1 | |
Active sitei | 370 | Proton acceptorSequence analysis | 1 | |
Metal bindingi | 370 | Magnesium 21 Publication | 1 | |
Sitei | 390 | Allosteric product binding, activate and inactive state | 1 |
GO - Molecular functioni
- diguanylate cyclase activity Source: UniProtKB-EC
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- phosphorelay signal transduction system Source: UniProtKB-KW
Keywordsi
Molecular function | Transducer, Transferase |
Biological process | Cell cycle, Differentiation, Two-component regulatory system |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00599 |
Names & Taxonomyi
Protein namesi | Recommended name: Response regulator PleDAlternative name(s): Stalked cell differentiation-controlling protein Including the following 1 domains: Alternative name(s): Diguanylate kinase |
Gene namesi | Name:pleD Ordered Locus Names:CCNA_02546 |
Organismi | Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus) |
Taxonomic identifieri | 565050 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Note: Phosphorylated PleD localizes to the differentiating pole.1 Publication
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000396955 | 1 – 454 | Response regulator PleDAdd BLAST | 454 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 53 | 4-aspartylphosphatePROSITE-ProRule annotation2 Publications | 1 |
Post-translational modificationi
Phosphorylated by PleC and DivJ. Phosphorylation stimulates cyclase activity.2 Publications
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Homodimer. Inactive monomer in solution, dimerization seems to be required for activity. Dimerization in vitro is enhanced by BeF3- and by 1 mM Mn2+ or 10 mM Mg2+.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | B8GZM2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | B8GZM2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 120 | Response regulatory 1PROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 155 – 269 | Response regulatory 2PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 319 – 454 | GGDEFPROSITE-ProRule annotationAdd BLAST | 136 |
Domaini
Activated by phosphorylation at the first response regulatory domain, which induces dimerization mediated by the two response regulatory domains and allows the two substrate-binding sites to approach each other and the condensation reaction to occur (Probable). The diguanylate cyclase activity is harbored by the GGDEF domain.Curated
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | CLU_000445_11_28_5 |
OMAi | NDYFVYP |
OrthoDBi | 1635706at2 |
Family and domain databases
CDDi | cd01949, GGDEF, 1 hit cd00156, REC, 1 hit |
Gene3Di | 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR000160, GGDEF_dom IPR029787, Nucleotide_cyclase IPR043128, Rev_trsase/Diguanyl_cyclase IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF00990, GGDEF, 1 hit PF00072, Response_reg, 2 hits |
SMARTi | View protein in SMART SM00267, GGDEF, 1 hit SM00448, REC, 2 hits |
SUPFAMi | SSF52172, SSF52172, 2 hits SSF55073, SSF55073, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit |
PROSITEi | View protein in PROSITE PS50887, GGDEF, 1 hit PS50110, RESPONSE_REGULATORY, 2 hits |
i Sequence
Sequence statusi: Complete.
B8GZM2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSARILVVDD IEANVRLLEA KLTAEYYEVS TAMDGPTALA MAARDLPDII
60 70 80 90 100
LLDVMMPGMD GFTVCRKLKD DPTTRHIPVV LITALDGRGD RIQGLESGAS
110 120 130 140 150
DFLTKPIDDV MLFARVRSLT RFKLVIDELR QREASGRRMG VIAGAAARLD
160 170 180 190 200
GLGGRVLIVD DNERQAQRVA AELGVEHRPV IESDPEKAKI SAGGPVDLVI
210 220 230 240 250
VNAAAKNFDG LRFTAALRSE ERTRQLPVLA MVDPDDRGRM VKALEIGVND
260 270 280 290 300
ILSRPIDPQE LSARVKTQIQ RKRYTDYLRN NLDHSLELAV TDQLTGLHNR
310 320 330 340 350
RYMTGQLDSL VKRATLGGDP VSALLIDIDF FKKINDTFGH DIGDEVLREF
360 370 380 390 400
ALRLASNVRA IDLPCRYGGE EFVVIMPDTA LADALRIAER IRMHVSGSPF
410 420 430 440 450
TVAHGREMLN VTISIGVSAT AGEGDTPEAL LKRADEGVYQ AKASGRNAVV
GKAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001340 Genomic DNA Translation: ACL96011.1 |
RefSeqi | WP_010920320.1, NC_011916.1 YP_002517919.1, NC_011916.1 |
Genome annotation databases
EnsemblBacteriai | ACL96011; ACL96011; CCNA_02546 |
GeneIDi | 7333643 |
KEGGi | ccs:CCNA_02546 |
PATRICi | fig|565050.3.peg.2497 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001340 Genomic DNA Translation: ACL96011.1 |
RefSeqi | WP_010920320.1, NC_011916.1 YP_002517919.1, NC_011916.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1W25 | X-ray | 2.70 | A/B | 2-454 | [»] | |
2V0N | X-ray | 2.71 | A/B | 2-454 | [»] | |
2WB4 | X-ray | 2.80 | A/B | 1-454 | [»] | |
SMRi | B8GZM2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
EnsemblBacteriai | ACL96011; ACL96011; CCNA_02546 |
GeneIDi | 7333643 |
KEGGi | ccs:CCNA_02546 |
PATRICi | fig|565050.3.peg.2497 |
Phylogenomic databases
HOGENOMi | CLU_000445_11_28_5 |
OMAi | NDYFVYP |
OrthoDBi | 1635706at2 |
Enzyme and pathway databases
UniPathwayi | UPA00599 |
Miscellaneous databases
EvolutionaryTracei | B8GZM2 |
Family and domain databases
CDDi | cd01949, GGDEF, 1 hit cd00156, REC, 1 hit |
Gene3Di | 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR000160, GGDEF_dom IPR029787, Nucleotide_cyclase IPR043128, Rev_trsase/Diguanyl_cyclase IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF00990, GGDEF, 1 hit PF00072, Response_reg, 2 hits |
SMARTi | View protein in SMART SM00267, GGDEF, 1 hit SM00448, REC, 2 hits |
SUPFAMi | SSF52172, SSF52172, 2 hits SSF55073, SSF55073, 1 hit |
TIGRFAMsi | TIGR00254, GGDEF, 1 hit |
PROSITEi | View protein in PROSITE PS50887, GGDEF, 1 hit PS50110, RESPONSE_REGULATORY, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLED_CAUVN | |
Accessioni | B8GZM2Primary (citable) accession number: B8GZM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 10, 2010 |
Last sequence update: | March 3, 2009 | |
Last modified: | December 2, 2020 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references