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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n) (Desulfurococcus kamchatkensis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADUniRule annotation1
Binding sitei62NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei127NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi157Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi172IronUniRule annotation1
Binding sitei222NAD; via amide nitrogenUniRule annotation1
Binding sitei232GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 55NADUniRule annotation2
Nucleotide bindingi155 – 157NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciDKAM490899:G1GCN-687-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:DKAM_0713Imported
OrganismiDesulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n) (Desulfurococcus kamchatkensis)Imported
Taxonomic identifieri490899 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus
Proteomesi
  • UP000006903 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi490899.DKAM_0713

Structurei

3D structure databases

ProteinModelPortaliB8D4K8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK03146
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

B8D4K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLESHITRV IWEEASRDWV ELSSCDIVVV GAGPSGLTAA KYLAEKGLKT
60 70 80 90 100
LVLERRLSFG GGIGGGGMLL HKTVVDERGL GILRDFNIRY KPSSIKGLYV
110 120 130 140 150
VDTAELTAKL AAGALDAGAK IIPGISVEDV IVRYNPFRVQ GVVVEWSAVQ
160 170 180 190 200
LSGLHVDPLF IESKAVIDAT GHDAEVLRIL EKKNPESKVK IPGEKSAYSE
210 220 230 240 250
KADVDVVEYT GRVIPGLYAT GMAVAAVRGL NRMGPIFTGM LLSGRKVAEA
260
VIRDLESAPK
Length:260
Mass (Da):27,823
Last modified:March 3, 2009 - v1
Checksum:i27165DCD8D3A33AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001140 Genomic DNA Translation: ACL11039.1
RefSeqiWP_012608380.1, NC_011766.1

Genome annotation databases

EnsemblBacteriaiACL11039; ACL11039; DKAM_0713
GeneIDi7170888
KEGGidka:DKAM_0713

Similar proteinsi

Entry informationi

Entry nameiB8D4K8_DESA1
AccessioniPrimary (citable) accession number: B8D4K8
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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