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UniProtKB - B8AKX6 (GLO1_ORYSI)
Protein
Glycolate oxidase 1
Gene
GLO1
Organism
Oryza sativa subsp. indica (Rice)
Status
Functioni
Catalyzes the oxidation of glycolate to glyoxylate, with a reduction of O2 to H2O2 (By similarity).
Is a key enzyme in photorespiration in plants (By similarity).
Can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Does not seem to play a role in oxalate accumulation (By similarity).
By similarityCatalytic activityi
Cofactori
FMNBy similarity
: photorespiration Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.By similarity This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 25 | GlyoxylateBy similarity | 1 | |
Binding sitei | 107 | FMNBy similarity | 1 | |
Sitei | 109 | Involved in determining the substrate specificity of glycolate oxidaseBy similarity | 1 | |
Binding sitei | 130 | GlyoxylateBy similarity | 1 | |
Binding sitei | 156 | FMNBy similarity | 1 | |
Binding sitei | 165 | GlyoxylateBy similarity | 1 | |
Binding sitei | 231 | FMNBy similarity | 1 | |
Binding sitei | 253 | FMNBy similarity | 1 | |
Active sitei | 255 | Proton acceptorBy similarity | 1 | |
Binding sitei | 255 | GlyoxylateBy similarity | 1 | |
Binding sitei | 258 | GlyoxylateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 78 – 80 | FMNBy similarity | 3 | |
Nucleotide bindingi | 128 – 130 | FMNBy similarity | 3 | |
Nucleotide bindingi | 286 – 290 | FMNBy similarity | 5 | |
Nucleotide bindingi | 309 – 310 | FMNBy similarity | 2 |
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: UniProtKB
- FMN binding Source: InterPro
GO - Biological processi
- cellular response to virus Source: UniProtKB
- oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
- photorespiration Source: UniProtKB
- regulation of photosynthesis Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolate pathway, Host-virus interaction, Photorespiration |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
UniPathwayi | UPA00951;UER00912 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycolate oxidase 1 (EC:1.1.3.15By similarity)Short name: GOX 1 Short name: OsGLO1 Alternative name(s): Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 Short chain alpha-hydroxy acid oxidase GLO1 |
Gene namesi | Name:GLO1 ORF Names:OsI_13800 |
Organismi | Oryza sativa subsp. indica (Rice) |
Taxonomic identifieri | 39946 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Peroxisome
- peroxisome Source: UniProtKB
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000403410 | 1 – 369 | Glycolate oxidase 1Add BLAST | 369 |
Proteomic databases
PRIDEi | B8AKX6 |
Interactioni
Subunit structurei
Homotetramer (By similarity).
Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 39946.B8AKX6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 360 | FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST | 360 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 367 – 369 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Phylogenomic databases
HOGENOMi | CLU_020639_0_0_1 |
OMAi | FDSGIRC |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
B8AKX6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGEITNVMEY QAIAKQKLPK MIYDYYASGA EDEWTLKENR EAFSRILFRP
60 70 80 90 100
RILIDVSKID MSATVLGFKI SMPIMIAPSA MQKMAHPDGE YATARAASAA
110 120 130 140 150
GTIMTLSSWA TSSVEEVAST GPGIRFFQLY VYKDRNVVEQ LVRRAERAGF
160 170 180 190 200
KAIALTVDTP RLGRREADIK NRFVLPPYLT LKNFEGLDLA EMDKSNDSGL
210 220 230 240 250
ASYVAGQIDR TLSWKDVKWL QSITSLPILV KGVITAEDAR LAVHSGAAGI
260 270 280 290 300
IVSNHGARQL DYVPATISAL EEVVTAAAGR IPVYLDGGVR RGTDVFKALA
310 320 330 340 350
LGAAGVFIGR PVVFALAAEG EAGVRNVLRM MREEFELTMA LSGCTSLADI
360
TRAHIYTDAD RLARPFPRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM000128 Genomic DNA Translation: EEC76290.1 |
Genome annotation databases
EnsemblPlantsi | BGIOSGA013686-TA; BGIOSGA013686-PA; BGIOSGA013686 |
Gramenei | BGIOSGA013686-TA; BGIOSGA013686-PA; BGIOSGA013686 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM000128 Genomic DNA Translation: EEC76290.1 |
3D structure databases
SMRi | B8AKX6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 39946.B8AKX6 |
Proteomic databases
PRIDEi | B8AKX6 |
Genome annotation databases
EnsemblPlantsi | BGIOSGA013686-TA; BGIOSGA013686-PA; BGIOSGA013686 |
Gramenei | BGIOSGA013686-TA; BGIOSGA013686-PA; BGIOSGA013686 |
Phylogenomic databases
HOGENOMi | CLU_020639_0_0_1 |
OMAi | FDSGIRC |
Enzyme and pathway databases
UniPathwayi | UPA00951;UER00912 |
Family and domain databases
CDDi | cd02809, alpha_hydroxyacid_oxid_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR012133, Alpha-hydoxy_acid_DH_FMN IPR000262, FMN-dep_DH IPR037396, FMN_HAD IPR008259, FMN_hydac_DH_AS |
Pfami | View protein in Pfam PF01070, FMN_dh, 1 hit |
PIRSFi | PIRSF000138, Al-hdrx_acd_dh, 1 hit |
PROSITEi | View protein in PROSITE PS00557, FMN_HYDROXY_ACID_DH_1, 1 hit PS51349, FMN_HYDROXY_ACID_DH_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLO1_ORYSI | |
Accessioni | B8AKX6Primary (citable) accession number: B8AKX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2011 |
Last sequence update: | March 3, 2009 | |
Last modified: | February 23, 2022 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families