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Protein

Glutathione synthetase

Gene
N/A
Organism
Homo sapiens (Human)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (GCLM), Glutamate--cysteine ligase catalytic subunit (GCLC)
  2. Glutathione synthetase, Glutathione synthetase (GSS), Glutathione synthetase (GSS), Glutathione synthetase, Glutathione synthetase (HEL-S-64p)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumUniRule annotation1
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei177ATPUniRule annotation1
Metal bindingi240MagnesiumUniRule annotation1
Binding sitei247ATPUniRule annotation1
Binding sitei297ATPUniRule annotation1
Binding sitei322SubstrateUniRule annotation1
Binding sitei324ATPUniRule annotation1
Binding sitei330ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi236 – 245ATPUniRule annotation10
Nucleotide bindingi270 – 273ATPUniRule annotation4

GO - Molecular functioni

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

PTM / Processingi

Proteomic databases

MaxQBiB7Z514
PeptideAtlasiB7Z514
PRIDEiB7Z514

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliB7Z514
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 174GSH_synthaseInterPro annotationAdd BLAST98

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
HOVERGENiHBG002458

Family and domain databases

Gene3Di3.30.1490.50, 1 hit
3.30.1490.80, 1 hit
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequencei

Sequence statusi: Complete.

B7Z514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQRSADGSP ALKQIEINTI SASFGGLASR TPAVHRHVLS VLSKTKEAGK
60 70 80 90 100
ILSNNPSKGL ALGIAKAWEL YGSPNALVLL IAQEKERNIF DQRAIENELL
110 120 130 140 150
ARNIHVIRRT FEDISEKGSL DQDRRLFVDG QEIAVVYFRD GYMPRQYSLQ
160 170 180 190 200
NWEARLLLER SHAAKCPDIA TQLAGTKKVQ QELSRPGMLE MLLPGQPEAV
210 220 230 240 250
ARLRATFAGL YSLDVGEEGD QAIAEALAAP SRFVLKPQRE GGGNNLYGEE
260 270 280 290 300
MVQALKQLKD SEERASYILM EKIEPEPFEN CLLRPGSPAR VVQCISELGI
310 320 330 340
FGVYVRQEKT LVMNKHVGHL LRTKAIEHAD GGVAAGVAVL DNPYPV
Length:346
Mass (Da):38,179
Last modified:March 3, 2009 - v1
Checksum:iDAC1ACDEDFD9A0FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK298292 mRNA Translation: BAH12750.1
UniGeneiHs.82327

Similar proteinsi

Entry informationi

Entry nameiB7Z514_HUMAN
AccessioniPrimary (citable) accession number: B7Z514
Entry historyiIntegrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: February 28, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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