UniProtKB - B7UN10 (MUKB_ECO27)
Protein
Chromosome partition protein MukB
Gene
mukB
Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Functioni
Plays a central role in chromosome condensation, segregation and cell cycle progression. Functions as a homodimer, which is essential for chromosome partition. Involved in negative DNA supercoiling in vivo, and by this means organize and compact chromosomes. May achieve or facilitate chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division.UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 41 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome condensation Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-UniRule
- DNA replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, Chromosome partition, DNA condensation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Chromosome partition protein MukBUniRule annotationAlternative name(s): Structural maintenance of chromosome-related proteinUniRule annotation |
Gene namesi | Name:mukBUniRule annotation Ordered Locus Names:E2348C_0917 |
Organismi | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
Taxonomic identifieri | 574521 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- nucleoid UniRule annotation Note: Restricted to the nucleoid region.UniRule annotation
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000187466 | 1 – 1486 | Chromosome partition protein MukBAdd BLAST | 1486 |
Interactioni
Subunit structurei
Homodimerization via its hinge domain. Binds to DNA via its C-terminal region.
Interacts, and probably forms a ternary complex, with MukE and MukF via its C-terminal region. The complex formation is stimulated by calcium or magnesium.
Interacts with tubulin-related protein FtsZ.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 666 – 783 | Flexible hingeUniRule annotationAdd BLAST | 118 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 326 – 418 | UniRule annotationAdd BLAST | 93 | |
Coiled coili | 444 – 480 | UniRule annotationAdd BLAST | 37 | |
Coiled coili | 509 – 603 | UniRule annotationAdd BLAST | 95 | |
Coiled coili | 835 – 923 | UniRule annotationAdd BLAST | 89 | |
Coiled coili | 977 – 1115 | UniRule annotationAdd BLAST | 139 | |
Coiled coili | 1209 – 1266 | UniRule annotationAdd BLAST | 58 |
Domaini
The hinge domain, which separates the large intramolecular coiled coil regions, allows the homodimerization, forming a V-shaped homodimer.UniRule annotation
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
HOGENOMi | CLU_004430_0_0_6 |
OMAi | FIAVYQH |
Family and domain databases
Gene3Di | 1.20.1060.30, 1 hit |
HAMAPi | MF_01800, MukB, 1 hit |
InterProi | View protein in InterPro IPR012090, MukB IPR032520, MukB_hinge IPR042501, MukB_hinge_sf IPR007406, MukB_N_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF04310, MukB, 1 hit PF16330, MukB_hinge, 1 hit |
PIRSFi | PIRSF005246, MukB, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
i Sequence
Sequence statusi: Complete.
B7UN10-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIERGKFRSL TLINWNGFFA RTFDLDELVT TLSGGNGAGK STTMAAFVTA
60 70 80 90 100
LIPDLTLLHF RNTTEAGATS GSRDKGLHGK LKAGVCYSML DTINSRHQRV
110 120 130 140 150
VVGVRLQQVA GRDRKVDIKP FAIQGLPMSV QPTQLVTETL NERQARVLPL
160 170 180 190 200
NELKDKLEAM EGVQFKQFNS ITDYHSLMFD LGIIARRLRS ASDRSKFYRL
210 220 230 240 250
IEASLYGGIS SAITRSLRDY LLPENSGVRK AFQDMEAALR ENRMTLEAIR
260 270 280 290 300
VTQSDRDLFK HLISEATNYV AADYMRHANE RRVHLDKALE FRRELHTSRQ
310 320 330 340 350
QLAAEQYKHV DMARELAEHN GAEGDLEADY QAASDHLNLV QTALRQQEKI
360 370 380 390 400
ERYEADLDEL QIRLEEQNEV VAEAIERQEE NEARAEAAEL EVDELKSQLA
410 420 430 440 450
DYQQALDVQQ TRAIQYNQAI AALNRAKELC HLPDLTADSA AEWLETFQAK
460 470 480 490 500
ELEATEKMLS LEQKMSMAQT AHSQFEQAYQ LVVAINGPLA RNEAWDVARE
510 520 530 540 550
LLREGVDQRH LAEQVQPLRM RLSELEQRLR EQQEAERLLA DFCKRQGKNF
560 570 580 590 600
DIDELEALHQ ELEARIASLS DSVSNAREER MALRQEQEQL QSRIQSLMQR
610 620 630 640 650
APVWLAAQNS LNQLSEQCGE EFTSSQDVTE FLQQLLERER EAIVERDEVG
660 670 680 690 700
ARKNAVDEEI ERLSQPGGSE DQRLNALAER FGGVLLSEIY DDVSLEDAPY
710 720 730 740 750
FSALYGPSRH AIVVPDLSQV TEHLEGLTDC PEDLYLIEGD PQSFDDSVFS
760 770 780 790 800
VDELEKAVVV KIADRQWRYS RFPEVPLFGR AARESRIESL HAEREVLSER
810 820 830 840 850
FATLSFDVQK IQRLHQAFSR FIGSHLAVAF ESDPEAEIRQ LNSRRVELER
860 870 880 890 900
ALSNHENDNQ QQRIQFEQAK EGVTALNRIL PRLNLLADDS LADRVDEIRE
910 920 930 940 950
RLDEAQEAAR FVQQFGNQLA KLEPIVSVLQ SDPEQFEQLK EDYAYSQQMQ
960 970 980 990 1000
RDARQQAFAL TEVVQRRAHF SYSDSAEMLS GNSDLNEKLR ERLEQAEAER
1010 1020 1030 1040 1050
TRAREALRGH AAQLNQYNQV LASLKSSYDT KKELLNDLQR ELQDIGVRAD
1060 1070 1080 1090 1100
SGAEERARIR RDELHAQLSN NRSRRNQLEK ALTFCEAEMD NLTRKLRKLE
1110 1120 1130 1140 1150
RDYFEMREQV VTAKAGWCAV MRMVKDNGVE RRLHRRELAY LSADDLRSMS
1160 1170 1180 1190 1200
DKALGALRLA VADNEHLRDV LRMSEDPKRP ERKIQFFVAV YQHLRERIRQ
1210 1220 1230 1240 1250
DIIRTDDPVE AIEQMEIELS RLTEELTSRE QKLAISSRSV ANIIRKTIQR
1260 1270 1280 1290 1300
EQNRIRMLNQ GLQNVSFGQV NSVRLNVNVR ETHAMLLDVL SEQHEQHQDL
1310 1320 1330 1340 1350
FNSNRLTFSE ALAKLYQRLN PQIDMGQRTP QTIGEELLDY RNYLEMEVEV
1360 1370 1380 1390 1400
NRGSDGWLRA ESGALSTGEA IGTGMSILVM VVQSWEDESR RLRGKDISPC
1410 1420 1430 1440 1450
RLLFLDEAAR LDARSIATLF ELCERLQMQL IIAAPENISP EKGTTYKLVR
1460 1470 1480
KVFQNTEHVH VVGLRGFAPQ LPETLPGSDE APSQAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS08465.1 |
RefSeqi | WP_000572652.1, NC_011601.1 |
Genome annotation databases
EnsemblBacteriai | CAS08465; CAS08465; E2348C_0917 |
KEGGi | ecg:E2348C_0917 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS08465.1 |
RefSeqi | WP_000572652.1, NC_011601.1 |
3D structure databases
SMRi | B7UN10 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | CAS08465; CAS08465; E2348C_0917 |
KEGGi | ecg:E2348C_0917 |
Phylogenomic databases
HOGENOMi | CLU_004430_0_0_6 |
OMAi | FIAVYQH |
Family and domain databases
Gene3Di | 1.20.1060.30, 1 hit |
HAMAPi | MF_01800, MukB, 1 hit |
InterProi | View protein in InterPro IPR012090, MukB IPR032520, MukB_hinge IPR042501, MukB_hinge_sf IPR007406, MukB_N_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF04310, MukB, 1 hit PF16330, MukB_hinge, 1 hit |
PIRSFi | PIRSF005246, MukB, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUKB_ECO27 | |
Accessioni | B7UN10Primary (citable) accession number: B7UN10 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | February 10, 2009 | |
Last modified: | December 2, 2020 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |