UniProtKB - B7UN08 (MUKF_ECO27)
Protein
Chromosome partition protein MukF
Gene
mukF
Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Functioni
Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.UniRule annotation
GO - Molecular functioni
- calcium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome condensation Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-UniRule
- DNA replication Source: UniProtKB-UniRule
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, DNA condensation |
Ligand | Calcium |
Names & Taxonomyi
Protein namesi | Recommended name: Chromosome partition protein MukFUniRule annotation |
Gene namesi | Name:mukFUniRule annotation Ordered Locus Names:E2348C_0915 |
Organismi | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
Taxonomic identifieri | 574521 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- nucleoid UniRule annotation Note: Restricted to the nucleoid region.UniRule annotation
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000187498 | 1 – 440 | Chromosome partition protein MukFAdd BLAST | 440 |
Interactioni
Subunit structurei
Interacts, and probably forms a ternary complex, with MukE and MukB via its C-terminal region. The complex formation is stimulated by calcium or magnesium. It is required for an interaction between MukE and MukB.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 208 – 236 | Leucine-zipperAdd BLAST | 29 |
Sequence similaritiesi
Belongs to the MukF family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_049853_0_0_6 |
OMAi | RIQDCVI |
Family and domain databases
CDDi | cd16337, MukF_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.10.225.40, 1 hit 1.20.58.590, 1 hit |
HAMAPi | MF_01803, MukF, 1 hit |
InterProi | View protein in InterPro IPR005582, Chromosome_partition_MukF IPR033441, MukF_C IPR038198, MukF_C_sf IPR033440, MukF_M IPR036141, MukF_M_sp IPR033439, MukF_WHTH IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF03882, KicB, 1 hit PF17193, MukF_C, 1 hit PF17192, MukF_M, 1 hit |
PIRSFi | PIRSF018282, MukF, 1 hit |
SUPFAMi | SSF140570, SSF140570, 1 hit SSF46785, SSF46785, 1 hit |
i Sequence
Sequence statusi: Complete.
B7UN08-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG
60 70 80 90 100
ELVDAFRHVS DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI
110 120 130 140 150
YRLTPLGIGI TDYYIRQREF STLRLSMQLS IVAGELKRAA DAAEEGGDEF
160 170 180 190 200
HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE QQQQVKDDIA QLLNKDWRAA
210 220 230 240 250
ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT MIHDDLHFVD
260 270 280 290 300
RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR
310 320 330 340 350
LRQSVQTYFD EPWALTYANA DRLLDMRDEE MVLRDEEVTG ELPEDLEYEE
360 370 380 390 400
FNEIREQLAA IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI
410 420 430 440
VIDQAVRLGV AQADFTGLPA KWQPINDYGA KVQAHVIDKY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS08463.1 |
RefSeqi | WP_001288839.1, NC_011601.1 |
Genome annotation databases
EnsemblBacteriai | CAS08463; CAS08463; E2348C_0915 |
KEGGi | ecg:E2348C_0915 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS08463.1 |
RefSeqi | WP_001288839.1, NC_011601.1 |
3D structure databases
SMRi | B7UN08 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | CAS08463; CAS08463; E2348C_0915 |
KEGGi | ecg:E2348C_0915 |
Phylogenomic databases
HOGENOMi | CLU_049853_0_0_6 |
OMAi | RIQDCVI |
Family and domain databases
CDDi | cd16337, MukF_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 1.10.225.40, 1 hit 1.20.58.590, 1 hit |
HAMAPi | MF_01803, MukF, 1 hit |
InterProi | View protein in InterPro IPR005582, Chromosome_partition_MukF IPR033441, MukF_C IPR038198, MukF_C_sf IPR033440, MukF_M IPR036141, MukF_M_sp IPR033439, MukF_WHTH IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF03882, KicB, 1 hit PF17193, MukF_C, 1 hit PF17192, MukF_M, 1 hit |
PIRSFi | PIRSF018282, MukF, 1 hit |
SUPFAMi | SSF140570, SSF140570, 1 hit SSF46785, SSF46785, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MUKF_ECO27 | |
Accessioni | B7UN08Primary (citable) accession number: B7UN08 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | February 10, 2009 | |
Last modified: | December 2, 2020 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |