UniProtKB - B7UMN5 (RHLB_ECO27)
Protein
ATP-dependent RNA helicase RhlB
Gene
rhlB
Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Functioni
DEAD-box RNA helicase involved in RNA degradation. Has RNA-dependent ATPase activity and unwinds double-stranded RNA.UniRule annotation
Catalytic activityi
- EC:3.6.4.13UniRule annotation
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 53 – 60 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- RNA binding Source: UniProtKB-UniRule
- RNA helicase activity Source: UniProtKB-UniRule
GO - Biological processi
- RNA catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rhlBUniRule annotation Ordered Locus Names:E2348C_4080 |
Organismi | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
Taxonomic identifieri | 574521 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000147580 | 1 – 421 | ATP-dependent RNA helicase RhlBAdd BLAST | 421 |
Interactioni
Subunit structurei
Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 219 | Helicase ATP-bindingUniRule annotationAdd BLAST | 180 | |
Domaini | 245 – 390 | Helicase C-terminalUniRule annotationAdd BLAST | 146 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 9 – 37 | Q motifAdd BLAST | 29 | |
Motifi | 165 – 168 | DEAD box | 4 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_003041_1_3_6 |
OMAi | RNPIRIL |
Family and domain databases
HAMAPi | MF_00661, DEAD_helicase_RhlB, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR023554, RNA_helicase_ATP-dep_RhlB IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
B7UMN5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA
60 70 80 90 100
GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI
110 120 130 140 150
HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK
160 170 180 190 200
QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT
210 220 230 240 250
LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL
260 270 280 290 300
IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD
310 320 330 340 350
EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA
360 370 380 390 400
GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT
410 420
RPRTGNGPRR TGAPRNRRRS G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS11628.1 |
RefSeqi | WP_000047499.1, NC_011601.1 |
Genome annotation databases
EnsemblBacteriai | CAS11628; CAS11628; E2348C_4080 |
GeneIDi | 49586958 |
KEGGi | ecg:E2348C_4080 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS11628.1 |
RefSeqi | WP_000047499.1, NC_011601.1 |
3D structure databases
SMRi | B7UMN5 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | CAS11628; CAS11628; E2348C_4080 |
GeneIDi | 49586958 |
KEGGi | ecg:E2348C_4080 |
Phylogenomic databases
HOGENOMi | CLU_003041_1_3_6 |
OMAi | RNPIRIL |
Family and domain databases
HAMAPi | MF_00661, DEAD_helicase_RhlB, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR023554, RNA_helicase_ATP-dep_RhlB IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHLB_ECO27 | |
Accessioni | B7UMN5Primary (citable) accession number: B7UMN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | February 10, 2009 | |
Last modified: | December 2, 2020 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |