UniProtKB - B7UM08 (DPS_ECO27)
Protein
DNA protection during starvation protein
Gene
dps
Organism
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Status
Functioni
During stationary phase, binds the chromosome non-specifically, forming a highly ordered and stable dps-DNA co-crystal within which chromosomal DNA is condensed and protected from diverse damages. It protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral, which can be released after reduction. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction. Dps also protects the cell from UV and gamma irradiation, iron and copper toxicity, thermal stress and acid and base shocks. Also shows a weak catalase activity.UniRule annotation
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 51 | Iron 1; shared with neighboring subunitUniRule annotation | 1 | |
Metal bindingi | 78 | Iron 1UniRule annotation | 1 | |
Metal bindingi | 82 | Iron 1UniRule annotation | 1 | |
Metal bindingi | 82 | Iron 2UniRule annotation | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-UniRule
- ferric iron binding Source: UniProtKB-UniRule
- oxidoreductase activity, oxidizing metal ions Source: InterPro
GO - Biological processi
- cellular iron ion homeostasis Source: UniProtKB-KW
- chromosome condensation Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Oxidoreductase |
Biological process | DNA condensation, Iron storage |
Ligand | Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: DNA protection during starvation proteinUniRule annotation (EC:1.16.-.-UniRule annotation) |
Gene namesi | Name:dpsUniRule annotation Ordered Locus Names:E2348C_0764 |
Organismi | Escherichia coli O127:H6 (strain E2348/69 / EPEC) |
Taxonomic identifieri | 574521 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- nucleoid UniRule annotation
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000184931 | 1 – 167 | DNA protection during starvation proteinAdd BLAST | 167 |
Interactioni
Subunit structurei
Homododecamer. The 12 subunits form a hollow sphere into which the mineral iron core of up to 500 Fe3+ can be deposited.
UniRule annotationFamily & Domainsi
Sequence similaritiesi
Belongs to the Dps family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_098183_1_2_6 |
OMAi | DDYSIGR |
Family and domain databases
CDDi | cd01043, DPS, 1 hit |
Gene3Di | 1.20.1260.10, 1 hit |
HAMAPi | MF_01441, Dps, 1 hit |
InterProi | View protein in InterPro IPR002177, DPS_DNA-bd IPR023188, DPS_DNA-bd_CS IPR023067, Dps_gammaproteobac IPR012347, Ferritin-like IPR009078, Ferritin-like_SF IPR008331, Ferritin_DPS_dom |
PANTHERi | PTHR42932, PTHR42932, 1 hit |
Pfami | View protein in Pfam PF00210, Ferritin, 1 hit |
PIRSFi | PIRSF005900, Dps, 1 hit |
PRINTSi | PR01346, HELNAPAPROT |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00818, DPS_1, 1 hit PS00819, DPS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
B7UM08-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTAKLVKSK ATNLLYTRND VSDSEKKATV ELLNRQVIQF IDLSLITKQA
60 70 80 90 100
HWNMRGANFI AVHEMLDGFR TALIDHLDTM AERAVQLGGV ALGTTQVINS
110 120 130 140 150
KTPLKSYPLD IHNVQDHLKE LADRYAIVAN DVRKAIGEAK DDDTADILTA
160
ASRDLDKFLW FIESNIE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS08312.1 |
RefSeqi | WP_000100800.1, NC_011601.1 |
Genome annotation databases
EnsemblBacteriai | CAS08312; CAS08312; E2348C_0764 |
GeneIDi | 52076120 |
KEGGi | ecg:E2348C_0764 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FM180568 Genomic DNA Translation: CAS08312.1 |
RefSeqi | WP_000100800.1, NC_011601.1 |
3D structure databases
SMRi | B7UM08 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | CAS08312; CAS08312; E2348C_0764 |
GeneIDi | 52076120 |
KEGGi | ecg:E2348C_0764 |
Phylogenomic databases
HOGENOMi | CLU_098183_1_2_6 |
OMAi | DDYSIGR |
Family and domain databases
CDDi | cd01043, DPS, 1 hit |
Gene3Di | 1.20.1260.10, 1 hit |
HAMAPi | MF_01441, Dps, 1 hit |
InterProi | View protein in InterPro IPR002177, DPS_DNA-bd IPR023188, DPS_DNA-bd_CS IPR023067, Dps_gammaproteobac IPR012347, Ferritin-like IPR009078, Ferritin-like_SF IPR008331, Ferritin_DPS_dom |
PANTHERi | PTHR42932, PTHR42932, 1 hit |
Pfami | View protein in Pfam PF00210, Ferritin, 1 hit |
PIRSFi | PIRSF005900, Dps, 1 hit |
PRINTSi | PR01346, HELNAPAPROT |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00818, DPS_1, 1 hit PS00819, DPS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPS_ECO27 | |
Accessioni | B7UM08Primary (citable) accession number: B7UM08 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | February 10, 2009 | |
Last modified: | December 2, 2020 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |