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UniProtKB - B7NEG2 (TREF_ECOLU)
Protein
Cytoplasmic trehalase
Gene
treF
Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Functioni
Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity.
UniRule annotationCatalytic activityi
- EC:3.2.1.28UniRule annotation
: trehalose degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glucose from alpha,alpha-trehalose.UniRule annotation This subpathway is part of the pathway trehalose degradation, which is itself part of Glycan degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose from alpha,alpha-trehalose, the pathway trehalose degradation and in Glycan degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 168 | SubstrateUniRule annotation | 1 | |
Binding sitei | 212 | SubstrateUniRule annotation | 1 | |
Binding sitei | 324 | Substrate; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 326 | Proton donor/acceptorUniRule annotation | 1 | |
Active sitei | 509 | Proton donor/acceptorUniRule annotation | 1 | |
Binding sitei | 525 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- alpha,alpha-trehalase activity Source: UniProtKB-UniRule
GO - Biological processi
- cellular hyperosmotic response Source: InterPro
- trehalose catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
UniPathwayi | UPA00300;UER00535 |
Protein family/group databases
CAZyi | GH37, Glycoside Hydrolase Family 37 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoplasmic trehalaseUniRule annotation (EC:3.2.1.28UniRule annotation)Alternative name(s): Alpha,alpha-trehalaseUniRule annotation Alpha,alpha-trehalose glucohydrolaseUniRule annotation |
Gene namesi | Name:treFUniRule annotation Ordered Locus Names:ECUMN_4020 |
Organismi | Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) |
Taxonomic identifieri | 585056 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000136404 | 1 – 549 | Cytoplasmic trehalaseAdd BLAST | 549 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 585056.ECUMN_4020 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 175 – 176 | Substrate bindingUniRule annotation | 2 | |
Regioni | 221 – 223 | Substrate bindingUniRule annotation | 3 | |
Regioni | 292 – 294 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 37 family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_006451_3_1_6 |
OMAi | DAPFGWA |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
HAMAPi | MF_01059, Cyt_trehalase, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR023715, Cyt_trehalase IPR001661, Glyco_hydro_37 IPR018232, Glyco_hydro_37_CS |
PANTHERi | PTHR23403, PTHR23403, 1 hit |
Pfami | View protein in Pfam PF01204, Trehalase, 1 hit |
PRINTSi | PR00744, GLHYDRLASE37 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00927, TREHALASE_1, 1 hit PS00928, TREHALASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
B7NEG2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNQKIQNPN PDELMIEVDL CYELDPYELK LDEMIEAEPE PEMIEGLPAS
60 70 80 90 100
DALTPADRYL ELFEHVQSAK IFPDSKTFPD CAPKMDPLDI LIRYRKVRRH
110 120 130 140 150
RDFDLRKFVE NHFWLPEVYS SEYVSDPQNS LKEHIDQLWP VLTREPQDHI
160 170 180 190 200
PWSSLLALPQ SYIVPGGRFS ETYYWDSYFT MLGLAESGRE DLLKCMADNF
210 220 230 240 250
AWMIENYGHI PNGNRTYYLS RSQPPVFALM VELFEEDGVR GARRYLDHLK
260 270 280 290 300
MEYAFWMDGA ESLIPNQAYR HVVRMPDGSL LNRYWDDRDT PRDESWLEDV
310 320 330 340 350
ETAKHSGRPP NEVYRDLRAG AASGWDYSSR WLRDTGRLAS IRTTQFIPID
360 370 380 390 400
LNAFLFKLES AIANISALKG EKETEALFRQ KASARRDAVN RYLWDDENGI
410 420 430 440 450
YRDYDWRREQ LALFSAAAIV PLYVGMANHE QADRLANAVR SRLLTPGGIL
460 470 480 490 500
ASEYETGEQW DKPNGWAPLQ WMAIQGFKMY GDDLLGDEIA RSWLKTVNQF
510 520 530 540
YLEQHKLIEK YHIADGVPRE GGGGEYPLQD GFGWTNGVVR RLIGLYGEP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU928163 Genomic DNA Translation: CAR15163.1 |
RefSeqi | WP_000934216.1, NC_011751.1 YP_002414667.1, NC_011751.1 |
Genome annotation databases
EnsemblBacteriai | CAR15163; CAR15163; ECUMN_4020 |
GeneIDi | 58462844 |
KEGGi | eum:ECUMN_4020 |
PATRICi | fig|585056.7.peg.4194 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU928163 Genomic DNA Translation: CAR15163.1 |
RefSeqi | WP_000934216.1, NC_011751.1 YP_002414667.1, NC_011751.1 |
3D structure databases
SMRi | B7NEG2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 585056.ECUMN_4020 |
Protein family/group databases
CAZyi | GH37, Glycoside Hydrolase Family 37 |
Genome annotation databases
EnsemblBacteriai | CAR15163; CAR15163; ECUMN_4020 |
GeneIDi | 58462844 |
KEGGi | eum:ECUMN_4020 |
PATRICi | fig|585056.7.peg.4194 |
Phylogenomic databases
HOGENOMi | CLU_006451_3_1_6 |
OMAi | DAPFGWA |
Enzyme and pathway databases
UniPathwayi | UPA00300;UER00535 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
HAMAPi | MF_01059, Cyt_trehalase, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR023715, Cyt_trehalase IPR001661, Glyco_hydro_37 IPR018232, Glyco_hydro_37_CS |
PANTHERi | PTHR23403, PTHR23403, 1 hit |
Pfami | View protein in Pfam PF01204, Trehalase, 1 hit |
PRINTSi | PR00744, GLHYDRLASE37 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00927, TREHALASE_1, 1 hit PS00928, TREHALASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TREF_ECOLU | |
Accessioni | B7NEG2Primary (citable) accession number: B7NEG2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 14, 2009 |
Last sequence update: | February 10, 2009 | |
Last modified: | February 23, 2022 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families