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Protein

UvrABC system protein B

Gene

uvrB

Organism
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:ECUMN_0921
OrganismiEscherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Taxonomic identifieri585056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000007097 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002005431 – 673UvrABC system protein BAdd BLAST673

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7NA78
SMRiB7NA78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 183Helicase ATP-bindingUniRule annotationAdd BLAST158
Domaini431 – 597Helicase C-terminalUniRule annotationAdd BLAST167
Domaini633 – 668UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 115Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073580
KOiK03702
OMAiRYMHSEI

Family and domain databases

CDDicd00079 HELICc, 1 hit
HAMAPiMF_00204 UvrB, 1 hit
InterProiView protein in InterPro
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR001943 UVR_dom
IPR036876 UVR_dom_sf
IPR004807 UvrB
IPR024759 UvrB_YAD/RRR_dom
PANTHERiPTHR24029 PTHR24029, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
PF02151 UVR, 1 hit
PF12344 UvrB, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF46600 SSF46600, 1 hit
SSF52540 SSF52540, 3 hits
TIGRFAMsiTIGR00631 uvrb, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50151 UVR, 1 hit

Sequencei

Sequence statusi: Complete.

B7NA78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA
60 70 80 90 100
NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPSSDTFIE KDASVNEHIE QMRLSATKAM LERRDVVVVA SVSAIYGLGD
160 170 180 190 200
PDLYLKMMLH LTVGMIIDQR AILRRLAELQ YARNDQAFQR GTFRVRGEVI
210 220 230 240 250
DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR FTIYPKTHYV
260 270 280 290 300
TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL
310 320 330 340 350
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM
360 370 380 390 400
YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE
410 420 430 440 450
LEKSGGDVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL
460 470 480 490 500
VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL
510 520 530 540 550
VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA
560 570 580 590 600
ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG
610 620 630 640 650
QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL
660 670
EFEEAAQIRD QLHQLRELFI AAS
Length:673
Mass (Da):76,226
Last modified:March 24, 2009 - v1
Checksum:i2F172045344FDAD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928163 Genomic DNA Translation: CAR12130.1
RefSeqiWP_000042533.1, NC_011751.1
YP_002411675.1, NC_011751.1

Genome annotation databases

EnsemblBacteriaiCAR12130; CAR12130; ECUMN_0921
GeneIDi7155879
KEGGieum:ECUMN_0921
PATRICifig|585056.7.peg.1116

Similar proteinsi

Entry informationi

Entry nameiUVRB_ECOLU
AccessioniPrimary (citable) accession number: B7NA78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 24, 2009
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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