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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277S-adenosyl-L-methionineUniRule annotation1
Binding sitei303S-adenosyl-L-methionineUniRule annotation1
Binding sitei322S-adenosyl-L-methionineUniRule annotation1
Active sitei375NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEFER585054:EFER_RS16445-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase BUniRule annotation (EC:2.1.1.176UniRule annotation)
Alternative name(s):
16S rRNA m5C967 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmBUniRule annotation
Gene namesi
Name:rsmBUniRule annotation
Synonyms:sunUniRule annotation
Ordered Locus Names:EFER_3272
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000745 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001886961 – 429Ribosomal RNA small subunit methyltransferase BAdd BLAST429

Proteomic databases

PRIDEiB7LRQ5

Structurei

3D structure databases

ProteinModelPortaliB7LRQ5
SMRiB7LRQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 260S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000037300
KOiK03500
OMAiLRVNRQH
OrthoDBiPOG091H02E2

Family and domain databases

Gene3Di1.10.940.10, 1 hit
HAMAPiMF_01856 16SrRNA_methyltr_B, 1 hit
InterProiView protein in InterPro
IPR018314 Fmu/NOL1/Nop2p_CS
IPR001678 MeTrfase_RsmB/NOP2
IPR035926 NusB-like_sf
IPR006027 NusB_RsmB_TIM44
IPR023267 RCMT
IPR004573 rRNA_ssu_MeTfrase_B
IPR023541 rRNA_ssu_MeTfrase_B_ent
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF01029 NusB, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF48013 SSF48013, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00563 rsmB, 1 hit
PROSITEiView protein in PROSITE
PS01153 NOL1_NOP2_SUN, 1 hit
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequencei

Sequence statusi: Complete.

B7LRQ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG
60 70 80 90 100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA
110 120 130 140 150
ETVEGAVAIK RPQLKGLING VLRQFQRQQE ELLAEFNASD ARYLHPSWLL
160 170 180 190 200
KRLQKAYPEQ WQSIIEANNQ RPPMWLRVNR THHSRDSWLA LLDEAGMKGF
210 220 230 240 250
PHADYPDAVR LETPAPVHVL PGFEEGWVTV QDASAQGCMA WLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA
360 370 380 390 400
QLQSEILDAI WPHLKSGGIL IYATCSVLPE ENSLQIKAFL QRTADAELCE
410 420
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Length:429
Mass (Da):48,358
Last modified:February 10, 2009 - v1
Checksum:i278AFB70DD42A5D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928158 Genomic DNA Translation: CAQ90752.1
RefSeqiWP_002431756.1, NC_011740.1

Genome annotation databases

EnsemblBacteriaiCAQ90752; CAQ90752; EFER_3272
KEGGiefe:EFER_3272

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928158 Genomic DNA Translation: CAQ90752.1
RefSeqiWP_002431756.1, NC_011740.1

3D structure databases

ProteinModelPortaliB7LRQ5
SMRiB7LRQ5
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB7LRQ5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ90752; CAQ90752; EFER_3272
KEGGiefe:EFER_3272

Phylogenomic databases

HOGENOMiHOG000037300
KOiK03500
OMAiLRVNRQH
OrthoDBiPOG091H02E2

Enzyme and pathway databases

BioCyciEFER585054:EFER_RS16445-MONOMER

Family and domain databases

Gene3Di1.10.940.10, 1 hit
HAMAPiMF_01856 16SrRNA_methyltr_B, 1 hit
InterProiView protein in InterPro
IPR018314 Fmu/NOL1/Nop2p_CS
IPR001678 MeTrfase_RsmB/NOP2
IPR035926 NusB-like_sf
IPR006027 NusB_RsmB_TIM44
IPR023267 RCMT
IPR004573 rRNA_ssu_MeTfrase_B
IPR023541 rRNA_ssu_MeTfrase_B_ent
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF01029 NusB, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF48013 SSF48013, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00563 rsmB, 1 hit
PROSITEiView protein in PROSITE
PS01153 NOL1_NOP2_SUN, 1 hit
PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSMB_ESCF3
AccessioniPrimary (citable) accession number: B7LRQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: October 10, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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