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Protein

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)

Gene

ispG

Organism
Bacillus cereus (strain AH820)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.UniRule annotation

Catalytic activityi

(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 5 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr), 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi268Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi271Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi303Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi310Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayi
UPA00056;UER00096

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)UniRule annotation (EC:1.17.7.3UniRule annotation)
Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthaseUniRule annotation
Gene namesi
Name:ispGUniRule annotation
Ordered Locus Names:BCAH820_4300
OrganismiBacillus cereus (strain AH820)
Taxonomic identifieri405535 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001363 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001181621 – 3704-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)Add BLAST370

Structurei

3D structure databases

ProteinModelPortaliB7JN03
SMRiB7JN03
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000261019
KOiK03526
OMAiDVHHNGM

Family and domain databases

Gene3Di3.20.20.20, 1 hit
HAMAPiMF_00159 IspG, 1 hit
InterProiView protein in InterPro
IPR011005 Dihydropteroate_synth-like
IPR016425 IspG_bac
IPR004588 IspG_bac-typ
PANTHERiPTHR30454 PTHR30454, 1 hit
PfamiView protein in Pfam
PF04551 GcpE, 1 hit
PIRSFiPIRSF004640 IspG, 1 hit
SUPFAMiSSF51717 SSF51717, 1 hit
TIGRFAMsiTIGR00612 ispG_gcpE, 1 hit

Sequencei

Sequence statusi: Complete.

B7JN03-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNEMTHRTKT RPVKVGNLTI GGNNELIIQS MTTTKTHDVE ATVAEIKRLE
60 70 80 90 100
EAGCQVVRVA VPDERAANAI ADIKKQINIP LVADIHFDYR LALKAIEGGI
110 120 130 140 150
DKVRINPGNI GRRHKVEAVV NAAKERGIPI RIGVNAGSLE RHILEKYGYP
160 170 180 190 200
TADGMVESAL HHIKILEDLD FHDIIVSMKA SDVNLAIEAY EKAARAFDYP
210 220 230 240 250
LHLGITESGT LFAGTVKSAA GLGAILNKGI GNTLRISLSA DPVEEVKVAR
260 270 280 290 300
ELLKSFGLAS NAATLISCPT CGRIEIDLIS IANEVEEYIS TLQVPIKVAV
310 320 330 340 350
LGCAVNGPGE AREADIGIAG ARGEGLLFRK GQVVRKVPEE IMVEELKKEI
360 370
DVIAAEMAAE REKEKETQEQ
Length:370
Mass (Da):40,027
Last modified:February 10, 2009 - v1
Checksum:i6F092C1658906340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001283 Genomic DNA Translation: ACK87719.1
RefSeqiWP_001004632.1, NC_011773.1

Genome annotation databases

EnsemblBacteriaiACK87719; ACK87719; BCAH820_4300
KEGGibcu:BCAH820_4300

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001283 Genomic DNA Translation: ACK87719.1
RefSeqiWP_001004632.1, NC_011773.1

3D structure databases

ProteinModelPortaliB7JN03
SMRiB7JN03
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK87719; ACK87719; BCAH820_4300
KEGGibcu:BCAH820_4300

Phylogenomic databases

HOGENOMiHOG000261019
KOiK03526
OMAiDVHHNGM

Enzyme and pathway databases

UniPathwayi
UPA00056;UER00096

Family and domain databases

Gene3Di3.20.20.20, 1 hit
HAMAPiMF_00159 IspG, 1 hit
InterProiView protein in InterPro
IPR011005 Dihydropteroate_synth-like
IPR016425 IspG_bac
IPR004588 IspG_bac-typ
PANTHERiPTHR30454 PTHR30454, 1 hit
PfamiView protein in Pfam
PF04551 GcpE, 1 hit
PIRSFiPIRSF004640 IspG, 1 hit
SUPFAMiSSF51717 SSF51717, 1 hit
TIGRFAMsiTIGR00612 ispG_gcpE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISPG_BACC0
AccessioniPrimary (citable) accession number: B7JN03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: October 10, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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