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Protein

Lipoyl synthase 2, mitochondrial

Gene

PHATRDRAFT_28652

Organism
Phaeodactylum tricornutum (strain CCAP 1055/1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase 1, mitochondrial (PHATRDRAFT_18029), Lipoyl synthase 2, mitochondrial (PHATRDRAFT_28652)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi57Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi72Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi76Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi79Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2, mitochondrial (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 2UniRule annotation
Short name:
LS 2UniRule annotation
Short name:
Lip-syn 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Gene namesi
ORF Names:PHATRDRAFT_28652
OrganismiPhaeodactylum tricornutum (strain CCAP 1055/1)
Taxonomic identifieri556484 [NCBI]
Taxonomic lineageiEukaryotaStramenopilesBacillariophytaBacillariophyceaeBacillariophycidaeNaviculalesPhaeodactylaceaePhaeodactylum
Proteomesi
  • UP000000759 Componentsi: Chromosome 13, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982441 – 314Lipoyl synthase 2, mitochondrialAdd BLAST314

Interactioni

Protein-protein interaction databases

STRINGi2850.Phatr28652

Structurei

3D structure databases

ProteinModelPortaliB7G3Y9
SMRiB7G3Y9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000235998
InParanoidiB7G3Y9
KOiK03644

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

B7G3Y9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPKGRPSWFK VPAPSQGTQS TYSTASYSRY AQVKDSLQKL DLHTVCEEAQ
60 70 80 90 100
CPNIGECWNG GTGTIMLLGD TCTRGCMFCA VNTDSKPPPP DPFEPFKTAE
110 120 130 140 150
AVVAWGVDYI VLTSVDRDDI ADGGAQHFAQ TVQLLKQNKP NLLVECLVSD
160 170 180 190 200
FQGMLDSVET LALSGLDVYA HNVETVERLQ PFVRDARANY QQSLSTLQHA
210 220 230 240 250
KVVKPELYTK TSIMLGLGET EEEVTQTMTD LRAIGVDVVT FGQYLRPTEH
260 270 280 290 300
HLSVVEYVTP EKFDHYRQVG ENMGFKYVAS GPMVRSSYKA GEFYLEHMIK
310
KERTEAAIDI GENA
Length:314
Mass (Da):34,862
Last modified:February 10, 2009 - v1
Checksum:i70E379E29A535340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000615 Genomic DNA Translation: EEC46905.1
RefSeqiXP_002181691.1, XM_002181655.1
UniGeneiPtc.241

Genome annotation databases

GeneIDi7202486
KEGGipti:PHATRDRAFT_28652

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000615 Genomic DNA Translation: EEC46905.1
RefSeqiXP_002181691.1, XM_002181655.1
UniGeneiPtc.241

3D structure databases

ProteinModelPortaliB7G3Y9
SMRiB7G3Y9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi2850.Phatr28652

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7202486
KEGGipti:PHATRDRAFT_28652

Phylogenomic databases

HOGENOMiHOG000235998
InParanoidiB7G3Y9
KOiK03644

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLIPA2_PHATC
AccessioniPrimary (citable) accession number: B7G3Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: February 10, 2009
Last modified: October 10, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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