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Protein

DNA ligase

Gene

ligA

Organism
Thermus aquaticus Y51MC23
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116NADUniRule annotation1
Active sitei118N6-AMP-lysine intermediateUniRule annotation1
Binding sitei139NADUniRule annotation1
Binding sitei174NADUniRule annotation1
Binding sitei291NADUniRule annotation1
Binding sitei315NADUniRule annotation1
Metal bindingi409ZincUniRule annotation1
Metal bindingi412ZincUniRule annotation1
Metal bindingi430ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 38NADUniRule annotation5
Nucleotide bindingi84 – 85NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processDNA damage, DNA repairUniRule annotation, DNA replicationUniRule annotation
LigandMagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
ORF Names:TaqDRAFT_4416Imported
OrganismiThermus aquaticus Y51MC23Imported
Taxonomic identifieri498848 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000005403 Componenti: Unassembled WGS sequence

Interactioni

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_4416

Structurei

3D structure databases

ProteinModelPortaliB7A6G7
SMRiB7A6G7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini586 – 664BRCTInterPro annotationAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili567 – 587Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105C77 Bacteria
COG0272 LUCA
KOiK01972
OrthoDBiPOG091H024G

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00114 LIGANc, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_01588 DNA_ligase_A, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR018239 DNA_ligase_AS
IPR033136 DNA_ligase_CS
IPR001679 DNAligase
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR003583 Hlx-hairpin-Hlx_DNA-bd_motif
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
PIRSFiPIRSF001604 LigA, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00278 HhH1, 4 hits
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00575 dnlj, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit

Sequencei

Sequence statusi: Complete.

B7A6G7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLEAARRRV NELRDLIRYH NYLYYVLDAP EISDAEYDRL LRELKELEER
60 70 80 90 100
FPELQSPDSP TEQVGARPLE STFRPVRHPT RMYSLDNAFS LDEVRAFEER
110 120 130 140 150
IERALGRKGP FLYTVEHKVD GLSVNLYYEE GILVFGATRG DGETGEEVTQ
160 170 180 190 200
NLLTIRTIPR RLTGVPDRLE VRGEVYMPIE AFLRLNQELE EAGERIFKNP
210 220 230 240 250
RNAAAGSLRQ KDPRVTARRG LRATFYALGL GLEETGLKSQ HDLLLWLRER
260 270 280 290 300
GFPVEHGFTR ALGAEGVEEV YQAWLKERRK LPFEADGVVV KLDDLALWRE
310 320 330 340 350
LGYTARAPRF ALAYKFPAEE KETRLLSVAF QVGRTGRITP VGVLEPVFIE
360 370 380 390 400
GSEVSRVTLH NESFIEELDV RIGDWVLVHK AGGVIPEVLR VLKERRTGEE
410 420 430 440 450
KPILWPENCP ECGHALIKEG KVHRCPNPLC PAKRFEAIRH YASRKAMDIQ
460 470 480 490 500
GLGEKLIEKL LEKGLVRDVA DLYRLKKEDL VNLERMGEKS AENLLRQIEE
510 520 530 540 550
SKGRGLERLL YALGLPGVGE VLARNLALRF GHMDRLLEAG LEDLLEVEGV
560 570 580 590 600
GELTARAILN TLKDPEFRDL VRRLKEAGVE MEAKEREGEA LKGLTFVITG
610 620 630 640 650
ELSRPREEVK ALLRRLGAKV TDSVSRKTGF LVVGENPGSK LEKARALGVP
660 670
TLSEEELYRL IEERTGKDPR ALTA
Length:674
Mass (Da):76,527
Last modified:February 10, 2009 - v1
Checksum:iBBE24C10B162EC56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABVK02000002 Genomic DNA Translation: EED10856.1
RefSeqiWP_003044913.1, NZ_CP010822.1

Genome annotation databases

EnsemblBacteriaiEED10856; EED10856; TaqDRAFT_4416
KEGGitaq:TO73_1739

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABVK02000002 Genomic DNA Translation: EED10856.1
RefSeqiWP_003044913.1, NZ_CP010822.1

3D structure databases

ProteinModelPortaliB7A6G7
SMRiB7A6G7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_4416

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEED10856; EED10856; TaqDRAFT_4416
KEGGitaq:TO73_1739

Phylogenomic databases

eggNOGiENOG4105C77 Bacteria
COG0272 LUCA
KOiK01972
OrthoDBiPOG091H024G

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00114 LIGANc, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_01588 DNA_ligase_A, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR018239 DNA_ligase_AS
IPR033136 DNA_ligase_CS
IPR001679 DNAligase
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR003583 Hlx-hairpin-Hlx_DNA-bd_motif
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
PIRSFiPIRSF001604 LigA, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00278 HhH1, 4 hits
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00575 dnlj, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiB7A6G7_THEAQ
AccessioniPrimary (citable) accession number: B7A6G7
Entry historyiIntegrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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