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UniProtKB - B6D1N4 (HPXO_KLEPN)
Protein
FAD-dependent urate hydroxylase
Gene
hpxO
Organism
Klebsiella pneumoniae
Status
Functioni
Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.
By similarityCatalytic activityi
- EC:1.14.13.113By similarity
Cofactori
FADBy similarity
: urate degradation Pathwayi
This protein is involved in the pathway urate degradation, which is part of Purine metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway urate degradation and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 43 | FADBy similarity | 1 | |
Binding sitei | 125 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 178 | SubstrateBy similarity | 1 | |
Binding sitei | 204 | SubstrateBy similarity | 1 | |
Sitei | 204 | Involved in substrate activation for the transfer of oxygen from the flavin hydroperoxideBy similarity | 1 | |
Binding sitei | 285 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 31 | FADBy similarity | 2 | |
Nucleotide bindingi | 295 – 299 | FADBy similarity | 5 |
GO - Molecular functioni
- FAD binding Source: UniProtKB
- FAD-dependent urate hydroxylase activity Source: UniProtKB-EC
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB
GO - Biological processi
- purine nucleobase metabolic process Source: UniProtKB-KW
- urate catabolic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Purine metabolism |
Ligand | FAD, Flavoprotein, NAD |
Enzyme and pathway databases
UniPathwayi | UPA00394 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hpxO |
Organismi | Klebsiella pneumoniae |
Taxonomic identifieri | 573 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella |
Pathology & Biotechi
Disruption phenotypei
Mutants show impaired utilization of both hypoxanthine and uric acid as nitrogen sources.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418847 | 1 – 384 | FAD-dependent urate hydroxylaseAdd BLAST | 384 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 216 – 218 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the FAD-dependent urate hydroxylase family.Curated
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
B6D1N4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAIVIGAGI GGLSAAVALK QSGIDCDVYE AVKEIKPVGA AISVWPNGVK
60 70 80 90 100
CMAHLGMGDI METFGGPLRR MAYRDFRSGE NMTQFSLAPL IERTGSRPCP
110 120 130 140 150
VSRAELQREM LDYWGRDSVQ FGKRVTRCEE DADGVTVWFT DGSSASGDLL
160 170 180 190 200
IAADGSHSAL RPWVLGFTPQ RRYAGYVNWN GLVEIDEALA PGDQWTTFVG
210 220 230 240 250
EGKRVSLMPV SAGRFYFFFD VPLPAGLAED RDTLRADLSR YFAGWAPPVQ
260 270 280 290 300
KLIAALDPQT TNRIEIHDIE PFSRLVRGRV ALLGDAGHST TPDIGQGGCA
310 320 330 340 350
AMEDAVVLGA VFRQTRDIAA ALCEYEAQRC DRVRDLVLKA RKRCDITHGK
360 370 380
DMQLTEAWYQ ELREETGERI INGMCDTILS GPLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU653284 Genomic DNA Translation: ACF60813.1 |
RefSeqi | WP_020804972.1, NZ_WULK01000014.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU653284 Genomic DNA Translation: ACF60813.1 |
RefSeqi | WP_020804972.1, NZ_WULK01000014.1 |
3D structure databases
SMRi | B6D1N4 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00394 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HPXO_KLEPN | |
Accessioni | B6D1N4Primary (citable) accession number: B6D1N4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | November 25, 2008 | |
Last modified: | February 23, 2022 | |
This is version 43 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families