UniProtKB - B6CJY4 (TRAF6_CERAT)
TNF receptor-associated factor 6
TRAF6
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 70 – 109 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 40 | |
Zinc fingeri | 150 – 202 | TRAF-type 1PROSITE-ProRule annotationAdd BLAST | 53 | |
Zinc fingeri | 203 – 259 | TRAF-type 2PROSITE-ProRule annotationAdd BLAST | 57 |
GO - Molecular functioni
- histone deacetylase binding Source: Ensembl
- identical protein binding Source: Ensembl
- mitogen-activated protein kinase kinase kinase binding Source: Ensembl
- protein kinase B binding Source: Ensembl
- protein N-terminus binding Source: Ensembl
- thioesterase binding Source: Ensembl
- tumor necrosis factor receptor binding Source: Ensembl
- ubiquitin conjugating enzyme binding Source: Ensembl
- ubiquitin protein ligase activity Source: Ensembl
- ubiquitin protein ligase binding Source: Ensembl
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- activation of NF-kappaB-inducing kinase activity Source: Ensembl
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Ensembl
- bone resorption Source: Ensembl
- cell development Source: Ensembl
- cellular response to DNA damage stimulus Source: UniProtKB-KW
- cellular response to lipopolysaccharide Source: Ensembl
- I-kappaB kinase/NF-kappaB signaling Source: Ensembl
- interleukin-1-mediated signaling pathway Source: Ensembl
- in utero embryonic development Source: Ensembl
- myeloid dendritic cell differentiation Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- neural tube closure Source: Ensembl
- odontogenesis of dentin-containing tooth Source: Ensembl
- ossification Source: Ensembl
- osteoclast differentiation Source: Ensembl
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of interleukin-12 production Source: Ensembl
- positive regulation of interleukin-2 production Source: Ensembl
- positive regulation of interleukin-6 production Source: Ensembl
- positive regulation of JUN kinase activity Source: Ensembl
- positive regulation of leukocyte adhesion to vascular endothelial cell Source: Ensembl
- positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of NIK/NF-kappaB signaling Source: Ensembl
- positive regulation of osteoclast differentiation Source: Ensembl
- positive regulation of T cell cytokine production Source: Ensembl
- positive regulation of T cell proliferation Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of transcription regulatory region DNA binding Source: Ensembl
- protein autoubiquitination Source: Ensembl
- protein K63-linked ubiquitination Source: UniProtKB
- regulation of apoptotic process Source: InterPro
- regulation of immunoglobulin secretion Source: Ensembl
- T cell receptor signaling pathway Source: Ensembl
- T-helper 1 type immune response Source: Ensembl
Keywordsi
Molecular function | Transferase |
Biological process | DNA damage, Immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: TNF receptor-associated factor 6 (EC:2.3.2.27)Alternative name(s): E3 ubiquitin-protein ligase TRAF6 RING-type E3 ubiquitin transferase TRAF6Curated |
Gene namesi | Name:TRAF6 |
Organismi | Cercocebus atys (Sooty mangabey) (Cercocebus torquatus atys) |
Taxonomic identifieri | 9531 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Cercocebus |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
- cell cortex By similarity
- Lipid droplet By similarity
Note: RSAD2/viperin recruits it to the lipid droplet.By similarity
Cytosol
- cytosol Source: Ensembl
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- CD40 receptor complex Source: Ensembl
- cytoplasmic side of plasma membrane Source: Ensembl
Other locations
- cell cortex Source: UniProtKB-SubCell
- lipid droplet Source: UniProtKB
- perinuclear region of cytoplasm Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, Lipid droplet, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000391608 | 1 – 522 | TNF receptor-associated factor 6Add BLAST | 522 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 124 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 124 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Cross-linki | 142 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 453 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationInteractioni
Subunit structurei
Homotrimer (By similarity). Homooligomer (By similarity). N-terminal region is dimeric while C-terminal region is trimeric; maybe providing a mode of oligomerization. Upon IL1B treatment, forms a complex with PELI1, IRAK1, IRAK4 and MYD88; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK2, IRAK3, RIPK2, MAP3K1, MAP3K5, MAP3K14, CSK, TRAF, TRAF-interacting protein TRIP and TNF receptor associated protein TDP2.
Interacts with IL17R.
Interacts with SQSTM1 bridging NTRK1 and NGFR.
Forms a ternary complex with SQSTM1 and PRKCZ.
Interacts with PELI2 and PELI3. Binds UBE2V1.
Interacts with MAVS/IPS1.
Interacts with TAX1BP1.
Interacts with IL1RL1.
Interacts with TRAFD1.
Interacts with ZNF675.
Interacts with AJUBA.
Interacts with TICAM2.
Interacts with ZFAND5.
Interacts with ARRB1 and ARRB2.
Interacts with MAP3K7 and TAB1/MAP3K7IP1; during IL-1 signaling.
Interacts with UBE2N.
Interacts with TGFBR1, HDAC1 and RANGAP1.
Interacts with AKT1, AKT2 and AKT3.
Interacts (via TRAF domains) with NUMBL (via C-terminal).
Interacts (via TRAF domains) with DYNC2I2 (via WD domains).
Interacts with RBCK1 (By similarity).
Interacts with LIMD1 (via LIM domains) (By similarity).
Interacts with RSAD2/viperin (By similarity).
Interacts with IFIT3 (via N-terminus) (By similarity).
Interacts (via C-terminus) with EIF2AK2/PKR (via the kinase catalytic domain).
Interacts with CARD14 (By similarity).
Interacts with CD40 and MAP3K8; the interaction is required for ERK activation.
Interacts with TICAM1 and this interaction is enhanced in the presence of WDFY1 (By similarity).
Interacts with TANK; this interaction increases in response to DNA damage (By similarity).
Interacts with USP10; this interaction increases in response to DNA damage (By similarity).
Interacts with ZC3H12A; this interaction increases in response to DNA damage and is stimulated by TANK (By similarity).
Interacts with WDFY3 (By similarity).
Interacts with GPS2 (By similarity).
Interacts (via C-terminus) with SASH1 (By similarity).
Interacts with LRRC19 (By similarity).
Interacts with IL17RA AND TRAF3IP2.
Interacts with TOMM70 (By similarity).
By similarityGO - Molecular functioni
- histone deacetylase binding Source: Ensembl
- identical protein binding Source: Ensembl
- mitogen-activated protein kinase kinase kinase binding Source: Ensembl
- protein kinase B binding Source: Ensembl
- protein N-terminus binding Source: Ensembl
- thioesterase binding Source: Ensembl
- tumor necrosis factor receptor binding Source: Ensembl
- ubiquitin conjugating enzyme binding Source: Ensembl
- ubiquitin protein ligase binding Source: Ensembl
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 350 – 499 | MATHPROSITE-ProRule annotationAdd BLAST | 150 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 354 | Interaction with TAX1BP1By similarityAdd BLAST | 354 | |
Regioni | 355 – 522 | Interaction with TANKBy similarityAdd BLAST | 168 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 288 – 348 | Sequence analysisAdd BLAST | 61 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 70 – 109 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 40 | |
Zinc fingeri | 150 – 202 | TRAF-type 1PROSITE-ProRule annotationAdd BLAST | 53 | |
Zinc fingeri | 203 – 259 | TRAF-type 2PROSITE-ProRule annotationAdd BLAST | 57 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerPhylogenomic databases
GeneTreei | ENSGT00940000155426 |
OMAi | ECAICID |
OrthoDBi | 918518at2759 |
Family and domain databases
CDDi | cd03776, MATH_TRAF6, 1 hit |
Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 3 hits |
InterProi | View protein in InterPro IPR002083, MATH/TRAF_dom IPR043211, TNF_rcpt-assoc_TRAF IPR012227, TNF_rcpt-assoc_TRAF_met IPR008974, TRAF-like IPR027139, TRAF6 IPR037309, TRAF6_MATH IPR041310, TRAF6_Z2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS IPR001293, Znf_TRAF |
PANTHERi | PTHR10131, PTHR10131, 1 hit PTHR10131:SF131, PTHR10131:SF131, 1 hit |
Pfami | View protein in Pfam PF18048, TRAF6_Z2, 1 hit PF02176, zf-TRAF, 1 hit |
PIRSFi | PIRSF015614, TRAF, 1 hit |
SMARTi | View protein in SMART SM00061, MATH, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF49599, SSF49599, 3 hits |
PROSITEi | View protein in PROSITE PS50144, MATH, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit PS50145, ZF_TRAF, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSLLNCENSC GSSQSESDCC VAMASSCSAA TKDDSVGGTA STGNLSSSFM
60 70 80 90 100
EEIQGYDVEF DPPLESKYEC PICLMALREA VQTPCGHRFC KACIIKSIRD
110 120 130 140 150
AGHKCPVDNE ILLENQLFPD NFAKREILSL MVKCPNEGCL HKMELRHLED
160 170 180 190 200
HQAHCEFALV DCPQCQRPFQ KFHINIHILK DCPRRQVSCD NCAALVAFED
210 220 230 240 250
KEIHDQNCPL ANVICEYCNT ILIREQMPNH YDLDCPTAPI PCTFSTFGCH
260 270 280 290 300
EKMQRNHLAR HLQENTQSHM RMLAQAVHSL SLIPDSGYVS EVRNFQETIH
310 320 330 340 350
QLEGRLVRQD HQIRELTAKM ETQSTYVSEL KRTIRTLEDK VAEIEAQQCN
360 370 380 390 400
GIYIWKIGNF GMHLKCQEEE KPVVIHSPGF YTGKPGYKLC MRLHLQLPTA
410 420 430 440 450
QRCANYISLF VHTMQGEYDS HLPWPFQGTI RLTILDQSEA PVRQNHEEIM
460 470 480 490 500
DAKPDLLAFQ RPTIPRNPKG FGYVTFMHLE ALRQRTFIKD DTLLVRCEVS
510 520
TRFDMGSLRR EGFQPRSTDS GV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU204928 mRNA Translation: ABY64982.1 |
RefSeqi | NP_001292890.1, NM_001305961.1 |
Genome annotation databases
Ensembli | ENSCATT00000037104; ENSCATP00000012971; ENSCATG00000030633 |
GeneIDi | 105586809 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU204928 mRNA Translation: ABY64982.1 |
RefSeqi | NP_001292890.1, NM_001305961.1 |
3D structure databases
BMRBi | B6CJY4 |
SMRi | B6CJY4 |
ModBasei | Search... |
Genome annotation databases
Ensembli | ENSCATT00000037104; ENSCATP00000012971; ENSCATG00000030633 |
GeneIDi | 105586809 |
Organism-specific databases
CTDi | 7189 |
Phylogenomic databases
GeneTreei | ENSGT00940000155426 |
OMAi | ECAICID |
OrthoDBi | 918518at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Family and domain databases
CDDi | cd03776, MATH_TRAF6, 1 hit |
Gene3Di | 2.60.210.10, 1 hit 3.30.40.10, 3 hits |
InterProi | View protein in InterPro IPR002083, MATH/TRAF_dom IPR043211, TNF_rcpt-assoc_TRAF IPR012227, TNF_rcpt-assoc_TRAF_met IPR008974, TRAF-like IPR027139, TRAF6 IPR037309, TRAF6_MATH IPR041310, TRAF6_Z2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS IPR001293, Znf_TRAF |
PANTHERi | PTHR10131, PTHR10131, 1 hit PTHR10131:SF131, PTHR10131:SF131, 1 hit |
Pfami | View protein in Pfam PF18048, TRAF6_Z2, 1 hit PF02176, zf-TRAF, 1 hit |
PIRSFi | PIRSF015614, TRAF, 1 hit |
SMARTi | View protein in SMART SM00061, MATH, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF49599, SSF49599, 3 hits |
PROSITEi | View protein in PROSITE PS50144, MATH, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit PS50145, ZF_TRAF, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRAF6_CERAT | |
Accessioni | B6CJY4Primary (citable) accession number: B6CJY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 9, 2010 |
Last sequence update: | November 25, 2008 | |
Last modified: | February 10, 2021 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families