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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58PotassiumBy similarity1
Metal bindingi123PotassiumBy similarity1
Binding sitei156NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi159PotassiumBy similarity1
Binding sitei329NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei447NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi417 – 421ADPBy similarity5
Nucleotide bindingi437 – 446ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciTYEL289376:G1GCQ-332-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:THEYE_A0332
OrganismiThermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)
Taxonomic identifieri289376 [NCBI]
Taxonomic lineageiBacteriaNitrospiraeNitrospiralesNitrospiraceaeThermodesulfovibrio
Proteomesi
  • UP000000718 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004164221 – 514Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST514

Interactioni

Protein-protein interaction databases

STRINGi289376.THEYE_A0332

Structurei

3D structure databases

ProteinModelPortaliB5YIM0
SMRiB5YIM0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 213YjeF N-terminalAdd BLAST203
Domaini223 – 506YjeF C-terminalAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 215NAD(P)H-hydrate epimeraseBy similarityAdd BLAST215
Regioni57 – 61NAD(P)HX (for epimerase activity)By similarity5
Regioni127 – 133NAD(P)HX (for epimerase activity)By similarity7
Regioni223 – 514ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST292
Regioni380 – 386NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
InParanoidiB5YIM0
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

B5YIM0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIMKIVTSHE MAEIDKLTID YYGIPSTVLM ERAALSIVKH VLKFNPENLI
60 70 80 90 100
ILAGPGNNGG DGIACGRILK NKVKNIKIFQ LFPEEKLSED CKLQLSVAKK
110 120 130 140 150
FGIPIVVGYP DNDQIDKADL IIDAIFGTGL KREIEGKVAE FIEILNSFKK
160 170 180 190 200
LTVAVDIPSG VSSDTGEILG AAIKANLTVT FGLPKRGHLL YPGKDYTGEL
210 220 230 240 250
FIEDIGFPEE LTNNENLKIS LIEREFALSL MPPRPKYSHK TRYGHVFVIA
260 270 280 290 300
GSTGKTGAAM MTAKAALRAG SGLVTMAVPA ALKVVFQSKV LEEMILPLPC
310 320 330 340 350
TMNTLSKQAL PEIMDFVKEK ANSIAFGPGV GVNEDIEIIL RELILNSSCP
360 370 380 390 400
IVIDADGLTV LSKILNVLKD AHSEIVLTPH PGELSRLINI PVKDIEKQRI
410 420 430 440 450
DIAQKVAQEL NVIIVLKGVP TVIAEPQGRV YLNTTGNPGM ATGGSGDVLT
460 470 480 490 500
GIIASLIGQG LSPLYASVFG VYIHGLSGDI ASRRKGFHGL IAGDIIETLP
510
EAFIELTDEI DSEP
Length:514
Mass (Da):55,363
Last modified:November 25, 2008 - v1
Checksum:iCE8BE1AD1829700F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA Translation: ACI20172.1
RefSeqiWP_012544910.1, NC_011296.1
YP_002248179.1, NC_011296.1

Genome annotation databases

EnsemblBacteriaiACI20172; ACI20172; THEYE_A0332
GeneIDi6942714
KEGGitye:THEYE_A0332
PATRICifig|289376.4.peg.325

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001147 Genomic DNA Translation: ACI20172.1
RefSeqiWP_012544910.1, NC_011296.1
YP_002248179.1, NC_011296.1

3D structure databases

ProteinModelPortaliB5YIM0
SMRiB5YIM0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi289376.THEYE_A0332

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACI20172; ACI20172; THEYE_A0332
GeneIDi6942714
KEGGitye:THEYE_A0332
PATRICifig|289376.4.peg.325

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
InParanoidiB5YIM0
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Enzyme and pathway databases

BioCyciTYEL289376:G1GCQ-332-MONOMER

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNR_THEYD
AccessioniPrimary (citable) accession number: B5YIM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: November 25, 2008
Last modified: October 10, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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