UniProtKB - B5X0E4 (ABCB5_MOUSE)
Protein
ATP-binding cassette sub-family B member 5
Gene
Abcb5
Organism
Mus musculus (Mouse)
Status
Functioni
Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 422 – 429 | ATPPROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1051 – 1058 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- efflux transmembrane transporter activity Source: MGI
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- eye development Source: UniProtKB
- regulation of membrane potential Source: MGI
- transmembrane transport Source: MGI
Keywordsi
Biological process | Differentiation, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-382556, ABC-family proteins mediated transport |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-binding cassette sub-family B member 5Alternative name(s): ABCB5 P-gp P-glycoprotein ABCB5 |
Gene namesi | Name:Abcb5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1924956, Abcb5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 46 – 66 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 104 – 124 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 290 – 310 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 314 – 334 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 694 – 714 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 738 – 758 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 814 – 836 | HelicalPROSITE-ProRule annotationAdd BLAST | 23 | |
Transmembranei | 841 – 863 | HelicalPROSITE-ProRule annotationAdd BLAST | 23 | |
Transmembranei | 955 – 975 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Defects in corneal development. While eyes contain all anterior and posterior segment components, corneas show developmental abnormalities characterized by decreased cellularity of the apical epithelial layer and disorganized basal and wing cell layers. Defects are due to depletion of quiescent limbal stem cells, leading to enhanced proliferation and apoptosis, and resulting in defective corneal differentiation and wound healing.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430433 | 1 – 1255 | ATP-binding cassette sub-family B member 5Add BLAST | 1255 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 86 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 189 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 372 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 391 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 643 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 790 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1036 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1105 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1189 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | B5X0E4 |
MaxQBi | B5X0E4 |
PaxDbi | B5X0E4 |
PRIDEi | B5X0E4 |
PTM databases
GlyGeni | B5X0E4, 11 sites |
iPTMneti | B5X0E4 |
PhosphoSitePlusi | B5X0E4 |
Expressioni
Tissue specificityi
In developing eye, expressed in basal limbal epithelium but not in central cornea. Acts as a marker of limbal stem cells.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000072791, Expressed in zygote and 14 other tissues |
Interactioni
Protein-protein interaction databases
BioGRIDi | 218862, 2 interactors |
IntActi | B5X0E4, 1 interactor |
STRINGi | 10090.ENSMUSP00000046177 |
Miscellaneous databases
RNActi | B5X0E4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 351 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 301 | |
Domaini | 387 – 623 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 237 | |
Domaini | 694 – 981 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 288 | |
Domaini | 1016 – 1254 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 239 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0055, Eukaryota |
GeneTreei | ENSGT00940000161340 |
HOGENOMi | CLU_000604_17_2_1 |
InParanoidi | B5X0E4 |
OMAi | QVGAQYH |
PhylomeDBi | B5X0E4 |
TreeFami | TF105193 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase IPR039421, Type_I_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: B5X0E4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MANSERTNGL QETNQRYGPL QEQVPKVGNQ AVGPIEIFRF ADNLDIVLMT
60 70 80 90 100
LGILASMING ATVPLMSLVL GEISDHLING CLVQTNRTKY QNCSQTQEKL
110 120 130 140 150
NEDIIVLTLY YIGIGAAALI FGYVQISFWV ITAARQTTRI RKQFFHSILA
160 170 180 190 200
QDISWFDGSD ICELNTRMTG DINKLCDGIG DKIPLMFQNI SGFSIGLVIS
210 220 230 240 250
LIKSWKLSLV VLSTSPLIMA SSALCSRMII SLTSKELDAY SKAGAVAEEA
260 270 280 290 300
LSSIQTVTAF GAQEKEIQRY TQHLKDAKDA GIKRATASKL SLGAVYFFMN
310 320 330 340 350
GAYGLAFWYG TSLIFGGEPG YTIGTILAVF FSVIHSSYCI GSVAPHLETF
360 370 380 390 400
TVARGAAFNI FQVIDKKPNI DNFSTAGFVP ECIEGNIEFK NVSFSYPSRP
410 420 430 440 450
SAKVLKGLNL KIKAGETVAL VGPSGSGKST TVQLLQRLYD PEDGCITVDE
460 470 480 490 500
NDIRAQNVRH YREQIGVVRQ EPVLFGTTIG NNIKFGREGV GEKEMEQAAR
510 520 530 540 550
EANAYDFIMA FPKKFNTLVG EKGAQMSGGQ KQRIAIARAL VRNPKILILD
560 570 580 590 600
EATSALDTES ESLVQTALEK ASKGRTTIVV AHRLSTIRGA DLIVTMKDGM
610 620 630 640 650
VVEKGTHAEL MAKQGLYYSL AMAQDIKKVD EQMESRTCST AGNASYGSLC
660 670 680 690 700
DVNSAKAPCT DQLEEAVHHQ KTSLPEVSLL KIFKLSKSEW PFVVLGTLAS
710 720 730 740 750
ALNGSVHPVF SIIFGKLVTM FEDKNKATLK QDAELYSMML VVLGIVALVT
760 770 780 790 800
YLMQGLFYGR AEENLAMRLR HSAFKAMLYQ DMAWYDDKEN NTGALTTTLA
810 820 830 840 850
VDVAQIQGAA TSRLGIVTQD VSNMSLSILI SFIYGWEMTL LILSFAPVLA
860 870 880 890 900
VTGMIQTAAM AGFANRDKQA LKRAGKIATE AVENIRTVVS LTRERAFEQM
910 920 930 940 950
YEETLQTQHR NALKRAHITG CCYAVSHAFV HFAHAAGFRF GAYLIQAGRM
960 970 980 990 1000
MPEGMFIVFT AIAYGAMAIG ETLVWAPEYS KAKAGASHLF ALLKNKPTIN
1010 1020 1030 1040 1050
SCSQSGEKPD TCEGNLEFRE VSFVYPCRPE VPVLQNMSLS IEKGKTVAFV
1060 1070 1080 1090 1100
GSSGCGKSTC VQLLQRFYDP MKGQVLLDGV DVKELNVQWL RSQTAIVSQE
1110 1120 1130 1140 1150
PVLFNCSIAE NIAYGDNSRM VPLEEIKEVA DAANIHSFIE GLPRKYNTLV
1160 1170 1180 1190 1200
GLRGVQLSGG QKQRLAIARA LLRKPKILLL DEATSALDNE SEKVVQQALD
1210 1220 1230 1240 1250
KARRGKTCLV VAHRLSTIQN ADMIVVLQNG SIKEQGTHQE LLRNGDTYFK
LVAAH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 564 | V → I in AFS60113 (PubMed:25030174).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056757 | 1 – 525 | Missing in isoform 2. 1 PublicationAdd BLAST | 525 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY766239 mRNA Translation: AAX11686.1 JQ655148 mRNA Translation: AFS60113.1 AC126277 Genomic DNA No translation available. AC131717 Genomic DNA No translation available. |
CCDSi | CCDS49198.1 [B5X0E4-1] |
RefSeqi | NP_084237.1, NM_029961.2 [B5X0E4-1] |
Genome annotation databases
Ensembli | ENSMUST00000035515; ENSMUSP00000046177; ENSMUSG00000072791 [B5X0E4-1] |
GeneIDi | 77706 |
KEGGi | mmu:77706 |
UCSCi | uc007pij.2, mouse [B5X0E4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY766239 mRNA Translation: AAX11686.1 JQ655148 mRNA Translation: AFS60113.1 AC126277 Genomic DNA No translation available. AC131717 Genomic DNA No translation available. |
CCDSi | CCDS49198.1 [B5X0E4-1] |
RefSeqi | NP_084237.1, NM_029961.2 [B5X0E4-1] |
3D structure databases
SMRi | B5X0E4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 218862, 2 interactors |
IntActi | B5X0E4, 1 interactor |
STRINGi | 10090.ENSMUSP00000046177 |
PTM databases
GlyGeni | B5X0E4, 11 sites |
iPTMneti | B5X0E4 |
PhosphoSitePlusi | B5X0E4 |
Proteomic databases
jPOSTi | B5X0E4 |
MaxQBi | B5X0E4 |
PaxDbi | B5X0E4 |
PRIDEi | B5X0E4 |
Protocols and materials databases
Antibodypediai | 11940, 392 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000035515; ENSMUSP00000046177; ENSMUSG00000072791 [B5X0E4-1] |
GeneIDi | 77706 |
KEGGi | mmu:77706 |
UCSCi | uc007pij.2, mouse [B5X0E4-1] |
Organism-specific databases
CTDi | 340273 |
MGIi | MGI:1924956, Abcb5 |
Phylogenomic databases
eggNOGi | KOG0055, Eukaryota |
GeneTreei | ENSGT00940000161340 |
HOGENOMi | CLU_000604_17_2_1 |
InParanoidi | B5X0E4 |
OMAi | QVGAQYH |
PhylomeDBi | B5X0E4 |
TreeFami | TF105193 |
Enzyme and pathway databases
Reactomei | R-MMU-382556, ABC-family proteins mediated transport |
Miscellaneous databases
BioGRID-ORCSi | 77706, 0 hits in 17 CRISPR screens |
PROi | PR:B5X0E4 |
RNActi | B5X0E4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000072791, Expressed in zygote and 14 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase IPR039421, Type_I_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCB5_MOUSE | |
Accessioni | B5X0E4Primary (citable) accession number: B5X0E4 Secondary accession number(s): W5QLL5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 2014 |
Last sequence update: | November 25, 2008 | |
Last modified: | December 2, 2020 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families