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Protein

ATP-binding cassette sub-family B member 5

Gene

Abcb5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi422 – 429ATPPROSITE-ProRule annotation8
Nucleotide bindingi1051 – 1058ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • compound eye corneal lens development Source: UniProtKB
  • regulation of membrane potential Source: MGI
  • transmembrane transport Source: MGI

Keywordsi

Biological processDifferentiation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family B member 5
Alternative name(s):
ABCB5 P-gp
P-glycoprotein ABCB5
Gene namesi
Name:Abcb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1924956 Abcb5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei46 – 66HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei104 – 124HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei290 – 310HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei314 – 334HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei694 – 714HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei738 – 758HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei814 – 836HelicalPROSITE-ProRule annotationAdd BLAST23
Transmembranei841 – 863HelicalPROSITE-ProRule annotationAdd BLAST23
Transmembranei955 – 975HelicalPROSITE-ProRule annotationAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Defects in corneal development. While eyes contain all anterior and posterior segment components, corneas show developmental abnormalities characterized by decreased cellularity of the apical epithelial layer and disorganized basal and wing cell layers. Defects are due to depletion of quiescent limbal stem cells, leading to enhanced proliferation and apoptosis, and resulting in defective corneal differentiation and wound healing.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004304331 – 1255ATP-binding cassette sub-family B member 5Add BLAST1255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi372N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi391N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi643N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi790N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1036N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1105N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1229N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiB5X0E4
PaxDbiB5X0E4
PRIDEiB5X0E4

PTM databases

iPTMnetiB5X0E4
PhosphoSitePlusiB5X0E4

Expressioni

Tissue specificityi

In developing eye, expressed in basal limbal epithelium but not in central cornea. Acts as a marker of limbal stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000072791 Expressed in 6 organ(s), highest expression level in female gonad

Interactioni

Protein-protein interaction databases

BioGridi218862, 1 interactor
IntActiB5X0E4, 1 interactor
STRINGi10090.ENSMUSP00000046177

Structurei

3D structure databases

ProteinModelPortaliB5X0E4
SMRiB5X0E4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 351ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST301
Domaini387 – 623ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini694 – 981ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST288
Domaini1016 – 1254ABC transporter 2PROSITE-ProRule annotationAdd BLAST239

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00530000062896
HOVERGENiHBG080809
InParanoidiB5X0E4
KOiK05660
OMAiGVFQAKM
OrthoDBiEOG091G0HVA
PhylomeDBiB5X0E4
TreeFamiTF105193

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR039421 Type_I_exporter
PANTHERiPTHR24221 PTHR24221, 2 hits
PfamiView protein in Pfam
PF00664 ABC_membrane, 2 hits
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
SSF90123 SSF90123, 2 hits
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 2 hits
PS00211 ABC_TRANSPORTER_1, 2 hits
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: B5X0E4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MANSERTNGL QETNQRYGPL QEQVPKVGNQ AVGPIEIFRF ADNLDIVLMT
60 70 80 90 100
LGILASMING ATVPLMSLVL GEISDHLING CLVQTNRTKY QNCSQTQEKL
110 120 130 140 150
NEDIIVLTLY YIGIGAAALI FGYVQISFWV ITAARQTTRI RKQFFHSILA
160 170 180 190 200
QDISWFDGSD ICELNTRMTG DINKLCDGIG DKIPLMFQNI SGFSIGLVIS
210 220 230 240 250
LIKSWKLSLV VLSTSPLIMA SSALCSRMII SLTSKELDAY SKAGAVAEEA
260 270 280 290 300
LSSIQTVTAF GAQEKEIQRY TQHLKDAKDA GIKRATASKL SLGAVYFFMN
310 320 330 340 350
GAYGLAFWYG TSLIFGGEPG YTIGTILAVF FSVIHSSYCI GSVAPHLETF
360 370 380 390 400
TVARGAAFNI FQVIDKKPNI DNFSTAGFVP ECIEGNIEFK NVSFSYPSRP
410 420 430 440 450
SAKVLKGLNL KIKAGETVAL VGPSGSGKST TVQLLQRLYD PEDGCITVDE
460 470 480 490 500
NDIRAQNVRH YREQIGVVRQ EPVLFGTTIG NNIKFGREGV GEKEMEQAAR
510 520 530 540 550
EANAYDFIMA FPKKFNTLVG EKGAQMSGGQ KQRIAIARAL VRNPKILILD
560 570 580 590 600
EATSALDTES ESLVQTALEK ASKGRTTIVV AHRLSTIRGA DLIVTMKDGM
610 620 630 640 650
VVEKGTHAEL MAKQGLYYSL AMAQDIKKVD EQMESRTCST AGNASYGSLC
660 670 680 690 700
DVNSAKAPCT DQLEEAVHHQ KTSLPEVSLL KIFKLSKSEW PFVVLGTLAS
710 720 730 740 750
ALNGSVHPVF SIIFGKLVTM FEDKNKATLK QDAELYSMML VVLGIVALVT
760 770 780 790 800
YLMQGLFYGR AEENLAMRLR HSAFKAMLYQ DMAWYDDKEN NTGALTTTLA
810 820 830 840 850
VDVAQIQGAA TSRLGIVTQD VSNMSLSILI SFIYGWEMTL LILSFAPVLA
860 870 880 890 900
VTGMIQTAAM AGFANRDKQA LKRAGKIATE AVENIRTVVS LTRERAFEQM
910 920 930 940 950
YEETLQTQHR NALKRAHITG CCYAVSHAFV HFAHAAGFRF GAYLIQAGRM
960 970 980 990 1000
MPEGMFIVFT AIAYGAMAIG ETLVWAPEYS KAKAGASHLF ALLKNKPTIN
1010 1020 1030 1040 1050
SCSQSGEKPD TCEGNLEFRE VSFVYPCRPE VPVLQNMSLS IEKGKTVAFV
1060 1070 1080 1090 1100
GSSGCGKSTC VQLLQRFYDP MKGQVLLDGV DVKELNVQWL RSQTAIVSQE
1110 1120 1130 1140 1150
PVLFNCSIAE NIAYGDNSRM VPLEEIKEVA DAANIHSFIE GLPRKYNTLV
1160 1170 1180 1190 1200
GLRGVQLSGG QKQRLAIARA LLRKPKILLL DEATSALDNE SEKVVQQALD
1210 1220 1230 1240 1250
KARRGKTCLV VAHRLSTIQN ADMIVVLQNG SIKEQGTHQE LLRNGDTYFK

LVAAH
Length:1,255
Mass (Da):137,397
Last modified:November 25, 2008 - v1
Checksum:i481BA90341116BC6
GO
Isoform 2 (identifier: B5X0E4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-525: Missing.

Show »
Length:730
Mass (Da):80,013
Checksum:i4FE7A189C3DA5ECA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti564V → I in AFS60113 (PubMed:25030174).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0567571 – 525Missing in isoform 2. 1 PublicationAdd BLAST525

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY766239 mRNA Translation: AAX11686.1
JQ655148 mRNA Translation: AFS60113.1
AC126277 Genomic DNA No translation available.
AC131717 Genomic DNA No translation available.
CCDSiCCDS49198.1 [B5X0E4-1]
RefSeqiNP_084237.1, NM_029961.2 [B5X0E4-1]
UniGeneiMm.261496

Genome annotation databases

EnsembliENSMUST00000035515; ENSMUSP00000046177; ENSMUSG00000072791 [B5X0E4-1]
GeneIDi77706
KEGGimmu:77706
UCSCiuc007pij.2 mouse [B5X0E4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY766239 mRNA Translation: AAX11686.1
JQ655148 mRNA Translation: AFS60113.1
AC126277 Genomic DNA No translation available.
AC131717 Genomic DNA No translation available.
CCDSiCCDS49198.1 [B5X0E4-1]
RefSeqiNP_084237.1, NM_029961.2 [B5X0E4-1]
UniGeneiMm.261496

3D structure databases

ProteinModelPortaliB5X0E4
SMRiB5X0E4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218862, 1 interactor
IntActiB5X0E4, 1 interactor
STRINGi10090.ENSMUSP00000046177

PTM databases

iPTMnetiB5X0E4
PhosphoSitePlusiB5X0E4

Proteomic databases

MaxQBiB5X0E4
PaxDbiB5X0E4
PRIDEiB5X0E4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035515; ENSMUSP00000046177; ENSMUSG00000072791 [B5X0E4-1]
GeneIDi77706
KEGGimmu:77706
UCSCiuc007pij.2 mouse [B5X0E4-1]

Organism-specific databases

CTDi340273
MGIiMGI:1924956 Abcb5

Phylogenomic databases

eggNOGiKOG0055 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00530000062896
HOVERGENiHBG080809
InParanoidiB5X0E4
KOiK05660
OMAiGVFQAKM
OrthoDBiEOG091G0HVA
PhylomeDBiB5X0E4
TreeFamiTF105193

Enzyme and pathway databases

ReactomeiR-MMU-382556 ABC-family proteins mediated transport

Miscellaneous databases

PROiPR:B5X0E4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072791 Expressed in 6 organ(s), highest expression level in female gonad

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR039421 Type_I_exporter
PANTHERiPTHR24221 PTHR24221, 2 hits
PfamiView protein in Pfam
PF00664 ABC_membrane, 2 hits
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
SSF90123 SSF90123, 2 hits
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 2 hits
PS00211 ABC_TRANSPORTER_1, 2 hits
PS50893 ABC_TRANSPORTER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiABCB5_MOUSE
AccessioniPrimary (citable) accession number: B5X0E4
Secondary accession number(s): W5QLL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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