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UniProtKB - B5GW45 (TMUUS_STRCL)
Protein
(+)-T-muurolol synthase ((2E,6E)-farnesyl diphosphate cyclizing)
Gene
SCLAV_p0068
Organism
Streptomyces clavuligerus
Status
Functioni
Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) into (+)-T-muurolol via a 1,10-cyclization, which requires isomerization of FPP to nerolidyl diphosphate (NPP) and then abstraction of the pyrophosphate from intermediate NPP leading to a (E,Z)-germacradienyl (helminthogermacradienyl) cation.
1 PublicationCatalytic activityi
- EC:4.2.3.981 Publication
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
Kineticsi
kcat is 0.00163 sec(-1).1 Publication
- KM=2.7 µM for (2E,6E)-farnesyl diphosphate1 Publication
: terpenoid biosynthesis Pathwayi
This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 80 | Plays a critical role in the stabilization of intermediate cationBy similarity | 1 | |
Metal bindingi | 83 | Magnesium 1By similarity | 1 | |
Sitei | 84 | Plays a critical role for substrate recognitionBy similarity | 1 | |
Metal bindingi | 88 | Magnesium 1By similarity | 1 | |
Metal bindingi | 88 | Magnesium 2By similarity | 1 | |
Sitei | 159 | Plays a critical role for substrate recognitionBy similarity | 1 | |
Binding sitei | 179 | SubstrateBy similarity | 1 | |
Sitei | 183 | Plays a critical role for abstraction of the pyrophosphate groupBy similarity | 1 | |
Metal bindingi | 225 | Magnesium 3By similarity | 1 | |
Metal bindingi | 229 | Magnesium 3By similarity | 1 | |
Binding sitei | 232 | SubstrateBy similarity | 1 | |
Metal bindingi | 233 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- lyase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- terpenoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 4.2.3.98, 5988 |
UniPathwayi | UPA00213 |
Names & Taxonomyi
Protein namesi | Recommended name: (+)-T-muurolol synthase ((2E,6E)-farnesyl diphosphate cyclizing)1 Publication (EC:4.2.3.981 Publication)Alternative name(s): Terpene cyclase1 Publication Type I terpene synthase1 Publication |
Gene namesi | ORF Names:SCLAV_p0068, SSCG_03688 |
Encoded oni | Plasmid pSCL41 Publication |
Organismi | Streptomyces clavuligerus |
Taxonomic identifieri | 1901 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418478 | 1 – 418 | (+)-T-muurolol synthase ((2E,6E)-farnesyl diphosphate cyclizing)Add BLAST | 418 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 312 – 313 | Substrate bindingBy similarity | 2 | |
Regioni | 354 – 418 | DisorderedSequence analysisAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 83 – 88 | DDXXXD motifBy similarity | 6 |
Domaini
The Asp-Asp-Xaa-Xaa-Xaa-Asp (DDXXXD) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity
Sequence similaritiesi
Belongs to the terpene synthase family.Curated
Phylogenomic databases
eggNOGi | COG3170, Bacteria |
OMAi | AMEHWVI |
OrthoDBi | 1869158at2 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034686, Terpene_cyclase-like_2 |
SFLDi | SFLDG01020, Terpene_Cyclase_Like_2, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
B5GW45-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLNHSDLMF YCPVDDLPHP AASGVNDRTL DWASGQGIPT ADRDAGRLRA
60 70 80 90 100
MAPGLLAARI APDARGPVLD AFADHHTWLF AFDDEYCDRA DGSGITEWAS
110 120 130 140 150
FLARLHRVVE TGESALLPGN PYGLALRDIA CRLSTYTTPA QLAEWLEALR
160 170 180 190 200
SYFAALVWER SRRRDDDRLQ SLDDYLLLRL RNGAMHTSIT LLDTVNGYVL
210 220 230 240 250
PRELRETPGV RALVEMTALL VSVDNDILSH HKESTSGTRE ANLLDVLGRT
260 270 280 290 300
GHTTPGEAVA QAVALRNEIM RQFVRVAERV RTPAAVPELY RFTTGLARWI
310 320 330 340 350
RANLDFSLTT TRYTGPVTER AALSPHEVPP LSGQGPAPAR SDVIGWWWRI
360 370 380 390 400
PEPLPEPGSD GADTPVRKRR AGDRPPTAGR GGAPHHQRTG PPPPVLPGGI
410
TASRSSGLQQ STWRREHR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM000914 Genomic DNA Translation: EFG03561.2 DS570654 Genomic DNA Translation: EDY50541.1 |
RefSeqi | WP_003956090.1, NZ_CP027859.1 |
Genome annotation databases
EnsemblBacteriai | EDY50541; EDY50541; SSCG_03688 |
KEGGi | ag:EFG03561 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM000914 Genomic DNA Translation: EFG03561.2 DS570654 Genomic DNA Translation: EDY50541.1 |
RefSeqi | WP_003956090.1, NZ_CP027859.1 |
3D structure databases
SMRi | B5GW45 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 443255.SCLAV_p0068 |
Genome annotation databases
EnsemblBacteriai | EDY50541; EDY50541; SSCG_03688 |
KEGGi | ag:EFG03561 |
Phylogenomic databases
eggNOGi | COG3170, Bacteria |
OMAi | AMEHWVI |
OrthoDBi | 1869158at2 |
Enzyme and pathway databases
UniPathwayi | UPA00213 |
BRENDAi | 4.2.3.98, 5988 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034686, Terpene_cyclase-like_2 |
SFLDi | SFLDG01020, Terpene_Cyclase_Like_2, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TMUUS_STRCL | |
Accessioni | B5GW45Primary (citable) accession number: B5GW45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | October 14, 2008 | |
Last modified: | September 29, 2021 | |
This is version 54 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Plasmid, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families