UniProtKB - B5DF07 (MRPP3_RAT)
Protein
Mitochondrial ribonuclease P catalytic subunit
Gene
Prorp
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalytic ribonuclease component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends. The presence of TRMT10C/MRPP1, HSD17B10/MRPP2 is required to catalyze tRNA molecules in their 5'-ends.By similarity
Catalytic activityi
- Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.By similarity EC:3.1.26.5
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 348 | ZincBy similarity | 1 | |
Metal bindingi | 351 | ZincBy similarity | 1 | |
Metal bindingi | 409 | Magnesium or manganese 1; catalyticBy similarity | 1 | |
Metal bindingi | 478 | Magnesium or manganese 1; catalyticBy similarity | 1 | |
Metal bindingi | 479 | Magnesium or manganese 2; catalyticBy similarity | 1 | |
Metal bindingi | 499 | Magnesium or manganese 2; catalyticBy similarity | 1 | |
Metal bindingi | 557 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 578 | ZincBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ribonuclease P activity Source: UniProtKB
GO - Biological processi
- mitochondrial tRNA 5'-end processing Source: UniProtKB
- tRNA 5'-leader removal Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Nuclease |
Biological process | tRNA processing |
Ligand | Magnesium, Manganese, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial ribonuclease P catalytic subunitBy similarity (EC:3.1.26.5By similarity)Alternative name(s): Mitochondrial ribonuclease P protein 3By similarity Short name: Mitochondrial RNase P protein 3By similarity |
Gene namesi | Name:Prorp Synonyms:Mrpp3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305089, RGD1305089 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrial nucleoid Source: RGD
- mitochondrial ribonuclease P complex Source: UniProtKB
- mitochondrion Source: RGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 46 | MitochondrionSequence analysisAdd BLAST | 46 | |
ChainiPRO_0000360398 | 47 – 587 | Mitochondrial ribonuclease P catalytic subunitAdd BLAST | 541 |
Post-translational modificationi
Degraded by LONP1 following mitochondrial unfolded protein response, probably leading to inhibit translation in mitochondrion.By similarity
Proteomic databases
PaxDbi | B5DF07 |
PeptideAtlasi | B5DF07 |
PRIDEi | B5DF07 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000007043, Expressed in testis and 22 other tissues |
Genevisiblei | B5DF07, RN |
Interactioni
Subunit structurei
Catalytic component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000009306 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 342 – 578 | PRORPAdd BLAST | 237 |
Domaini
Displays a distorted and non-productive active site that probably switches to a fully productive state only upon association with TRMT10C/MRPP1, HSD17B10/MRPP2 and pre-tRNA substrate.By similarity
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | ENOG502QRKG, Eukaryota |
GeneTreei | ENSGT00390000002201 |
HOGENOMi | CLU_033070_1_0_1 |
InParanoidi | B5DF07 |
OMAi | APYDCVI |
OrthoDBi | 1247650at2759 |
PhylomeDBi | B5DF07 |
TreeFami | TF324726 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR033495, MRPP3 IPR031595, PRORP_C IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR13547:SF1, PTHR13547:SF1, 1 hit |
Pfami | View protein in Pfam PF16953, PRORP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
B5DF07-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTFYLSGFRS FLKLWKSNPY FELGPATSSA SFFLGVHCVI GNQQRWFSVK
60 70 80 90 100
PTTPPNSKIL NLLVTKARTL KKGNDSNKQA SSGPHYFAAG EAKKRSHLSG
110 120 130 140 150
NPQNQGHTLP VKSTVQLPTK PLNSEEWDKL KEDFKGKASF EDFVISQMTR
160 170 180 190 200
SCSSVDVAKS LLAWVAAKNN GIVGYNLLVK YLYLCVFHKQ TSEVIDVYEI
210 220 230 240 250
MKAKYKSLES GGYTLLIRGL IHSDRWREAL LLLEDIKKVM VPSKKNYGDC
260 270 280 290 300
IQGALLHQDV NVAWSLYQEL VGHNLIPLLE TLKAFFDHGK DMNDDQYSNQ
310 320 330 340 350
LLDILLYLRN NQLYPGESFA HSIKTWFESI PGRQWKGQFT TIQKSGQCSS
360 370 380 390 400
CGRAIESIHL SPEEYEFLKE TIMRDVIDGG DQYKKTTPQE LKRFERFVKS
410 420 430 440 450
CPPFDIVIDG LNVAKMFPKG RESQNLLGIV SQLAQQNLQL LVLGRKHMLR
460 470 480 490 500
PSSQWRKDEM EQVRKQAHCF FADNISEDDP FLLYATLNSG SHCKFITKDL
510 520 530 540 550
LRDHKACLPD ARAQRLFFKW QQGHQLAITK GFLKSKLTFQ HILSYDTVVQ
560 570 580
TTGDTWHIPY DEDLVPRSSC EVPTKWLCLQ RKTPAPC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473947 Genomic DNA Translation: EDM03424.1 BC168878 mRNA Translation: AAI68878.1 |
RefSeqi | NP_001100200.1, NM_001106730.2 |
Genome annotation databases
Ensembli | ENSRNOT00000009304; ENSRNOP00000009306; ENSRNOG00000007043 |
GeneIDi | 299050 |
KEGGi | rno:299050 |
UCSCi | RGD:1305089, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473947 Genomic DNA Translation: EDM03424.1 BC168878 mRNA Translation: AAI68878.1 |
RefSeqi | NP_001100200.1, NM_001106730.2 |
3D structure databases
SMRi | B5DF07 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000009306 |
Proteomic databases
PaxDbi | B5DF07 |
PeptideAtlasi | B5DF07 |
PRIDEi | B5DF07 |
Genome annotation databases
Ensembli | ENSRNOT00000009304; ENSRNOP00000009306; ENSRNOG00000007043 |
GeneIDi | 299050 |
KEGGi | rno:299050 |
UCSCi | RGD:1305089, rat |
Organism-specific databases
CTDi | 9692 |
RGDi | 1305089, RGD1305089 |
Phylogenomic databases
eggNOGi | ENOG502QRKG, Eukaryota |
GeneTreei | ENSGT00390000002201 |
HOGENOMi | CLU_033070_1_0_1 |
InParanoidi | B5DF07 |
OMAi | APYDCVI |
OrthoDBi | 1247650at2759 |
PhylomeDBi | B5DF07 |
TreeFami | TF324726 |
Miscellaneous databases
PROi | PR:B5DF07 |
Gene expression databases
Bgeei | ENSRNOG00000007043, Expressed in testis and 22 other tissues |
Genevisiblei | B5DF07, RN |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR033495, MRPP3 IPR031595, PRORP_C IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR13547:SF1, PTHR13547:SF1, 1 hit |
Pfami | View protein in Pfam PF16953, PRORP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MRPP3_RAT | |
Accessioni | B5DF07Primary (citable) accession number: B5DF07 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | October 14, 2008 | |
Last modified: | December 2, 2020 | |
This is version 67 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families