Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 83 (17 Jun 2020)
Sequence version 1 (14 Oct 2008)
Previous versions | rss
Help videoAdd a publicationFeedback
Protein

Thiamine pyrophosphokinase

Gene

Tpk1

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase (Tpk1)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinaseUniRule annotation, Transferase
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-RNO-196819 Vitamin B1 (thiamin) metabolism

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00060;UER00597

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Thiamine pyrophosphokinaseUniRule annotation (EC:2.7.6.2UniRule annotation)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Tpk1Imported
ORF Names:rCG_28258Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4

Organism-specific databases

Rat genome database

More...
RGDi
1589408 Tpk1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSRNOG00000029922 Expressed in adult mammalian kidney and 8 other tissues

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
10116.ENSRNOP00000044768

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini168 – 235TPK_B1_bindingInterPro annotationAdd BLAST68

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3153 Eukaryota
COG1564 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000016016

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_044237_0_1_1

KEGG Orthology (KO)

More...
KOi
K00949

Identification of Orthologs from Complete Genome Data

More...
OMAi
QMEFGGM

Database of Orthologous Groups

More...
OrthoDBi
1428589at2759

TreeFam database of animal gene trees

More...
TreeFami
TF313224

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07995 TPK, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.10240, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006282 Thi_PPkinase
IPR016966 Thiamin_pyrophosphokinase_euk
IPR007373 Thiamin_PyroPKinase_B1-bd
IPR036371 TPK_B1-bd_sf
IPR007371 TPK_catalytic
IPR036759 TPK_catalytic_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04265 TPK_B1_binding, 1 hit
PF04263 TPK_catalytic, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF031057 Thiamin_pyrophosphokinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00983 TPK_B1_binding, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF63862 SSF63862, 1 hit
SSF63999 SSF63999, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01378 thi_PPkinase, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

B5DEJ6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEHVFTPLEP LLPTGDLKFC LVVLNQTLDP HFRHLWRKAL LRACADGGAN
60 70 80 90 100
HLYDLTEGER ESFLPEFING DFDSIRPEVK EYYTKKGCDL ISTPDQDHTD
110 120 130 140 150
FTKCLQVLQR KIEEKELQVD VIVTLGGLGG RFDQIMASVN TLFQATDIIP
160 170 180 190 200
VPIIIIQKES LIYLLQPGKH RLRVDTGMEG SWCGLIPVGQ PCNHVTTTGL
210 220 230 240
KWNLTNDVLG FGTLVSTSNT YDGSGLVTVE TDHPLLWTMA IKT
Length:243
Mass (Da):27,207
Last modified:October 14, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB12C148D16B2CA14
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AABR07060411 Genomic DNA No translation available.
AABR07060412 Genomic DNA No translation available.
AABR07060413 Genomic DNA No translation available.
AABR07060414 Genomic DNA No translation available.
AABR07060415 Genomic DNA No translation available.
AABR07060416 Genomic DNA No translation available.
AABR07060417 Genomic DNA No translation available.
AABR07060418 Genomic DNA No translation available.
AABR07060419 Genomic DNA No translation available.
AABR07060420 Genomic DNA No translation available.
BC168695 mRNA Translation: AAI68695.1
CH473959 Genomic DNA Translation: EDM15544.1

NCBI Reference Sequences

More...
RefSeqi
NP_001128466.1, NM_001134994.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSRNOT00000048203; ENSRNOP00000044768; ENSRNOG00000029922

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
680668

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
rno:680668

UCSC genome browser

More...
UCSCi
RGD:1589408 rat

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR07060411 Genomic DNA No translation available.
AABR07060412 Genomic DNA No translation available.
AABR07060413 Genomic DNA No translation available.
AABR07060414 Genomic DNA No translation available.
AABR07060415 Genomic DNA No translation available.
AABR07060416 Genomic DNA No translation available.
AABR07060417 Genomic DNA No translation available.
AABR07060418 Genomic DNA No translation available.
AABR07060419 Genomic DNA No translation available.
AABR07060420 Genomic DNA No translation available.
BC168695 mRNA Translation: AAI68695.1
CH473959 Genomic DNA Translation: EDM15544.1
RefSeqiNP_001128466.1, NM_001134994.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044768

Genome annotation databases

EnsembliENSRNOT00000048203; ENSRNOP00000044768; ENSRNOG00000029922
GeneIDi680668
KEGGirno:680668
UCSCiRGD:1589408 rat

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
27010
RGDi1589408 Tpk1

Phylogenomic databases

eggNOGiKOG3153 Eukaryota
COG1564 LUCA
GeneTreeiENSGT00390000016016
HOGENOMiCLU_044237_0_1_1
KOiK00949
OMAiQMEFGGM
OrthoDBi1428589at2759
TreeFamiTF313224

Enzyme and pathway databases

UniPathwayiUPA00060;UER00597
ReactomeiR-RNO-196819 Vitamin B1 (thiamin) metabolism

Gene expression databases

BgeeiENSRNOG00000029922 Expressed in adult mammalian kidney and 8 other tissues

Family and domain databases

CDDicd07995 TPK, 1 hit
Gene3Di3.40.50.10240, 1 hit
InterProiView protein in InterPro
IPR006282 Thi_PPkinase
IPR016966 Thiamin_pyrophosphokinase_euk
IPR007373 Thiamin_PyroPKinase_B1-bd
IPR036371 TPK_B1-bd_sf
IPR007371 TPK_catalytic
IPR036759 TPK_catalytic_sf
PfamiView protein in Pfam
PF04265 TPK_B1_binding, 1 hit
PF04263 TPK_catalytic, 1 hit
PIRSFiPIRSF031057 Thiamin_pyrophosphokinase, 1 hit
SMARTiView protein in SMART
SM00983 TPK_B1_binding, 1 hit
SUPFAMiSSF63862 SSF63862, 1 hit
SSF63999 SSF63999, 1 hit
TIGRFAMsiTIGR01378 thi_PPkinase, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiB5DEJ6_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: B5DEJ6
Secondary accession number(s): F1LQE3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: October 14, 2008
Last sequence update: October 14, 2008
Last modified: June 17, 2020
This is version 83 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Proteomics identificationCombined sources, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again