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Protein

Wilms tumor protein homolog

Gene

wt1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription factor required for development of the vascular component of the pronephric kidney, the glomus; may repress tubule-specific gene expression in the portion of the pronephros fated to form the glomus (By similarity). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (By similarity). Inhibits Wnt-signaling during embryonic development (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei388Important for interaction with target DNABy similarity1
Sitei394Important for interaction with target DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri290 – 314C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri320 – 344C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri350 – 372C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri378 – 402C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, RNA-binding
Biological processTranscription, Transcription regulation, Wnt signaling pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Wilms tumor protein homologBy similarity
Gene namesi
Name:wt1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-484999 wt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003913891 – 413Wilms tumor protein homologAdd BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki159Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiB5DE03

Structurei

3D structure databases

SMRiB5DE03
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni334 – 348Important for interaction with target DNABy similarityAdd BLAST15
Regioni360 – 368Important for interaction with target DNABy similarity9

Domaini

Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers. Starting from the N-terminus, the second zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine, 5-formylcytosine or 5-carboxylcytosine.By similarity

Sequence similaritiesi

Belongs to the EGR C2H2-type zinc-finger protein family.Sequence analysis

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri290 – 314C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri320 – 344C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri350 – 372C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri378 – 402C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000230937
HOVERGENiHBG006960
InParanoidiB5DE03
KOiK09234

Family and domain databases

InterProiView protein in InterPro
IPR017987 Wilms_tumour
IPR000976 Wilms_tumour_N
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44601 PTHR44601, 1 hit
PfamiView protein in Pfam
PF02165 WT1, 1 hit
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 4 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

B5DE03-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSDVRDMNA LLPPVSTLSG NSSCSMPVSS SGQWAPVLDF PPGAPYSSLP
60 70 80 90 100
PHSFIKQEPT WNPDPHEDQC LSAFTVHFSG QFTGTAGACR YGAFGAPTPS
110 120 130 140 150
QATTGQARMF PNSPYLSNCL DNQQGMRNQG YSAVAFDGTP SYGHTPSHHT
160 170 180 190 200
AQFTNHSFKH EDPMGQQTSL GEQQYSVPPP VYGCHTPTDS CTGSQALLLR
210 220 230 240 250
TPYNSDNLYQ MTSQLECMTW NQMNLGSSLK SHGTSYENDS HSTPMLYSCG
260 270 280 290 300
GQYRIHTHGV FRGIQDVRRV PGVTPAIVRS STEANEKRPF MCAYPGCNKR
310 320 330 340 350
YFKLSHLQMH SRKHTGEKPY QCDFKDCERR FSRSDQLKRH QRRHTGIKPF
360 370 380 390 400
QCKTCQRKFS RSDHLKTHTR THTGEKPFSC RWPSCQKKFA RSDELVRHHN
410
MHQRNMTKLQ LAL
Length:413
Mass (Da):46,461
Last modified:October 14, 2008 - v1
Checksum:iCF62CB23A13C58A8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6SKU4F6SKU4_XENTR
Wilms tumor protein homolog
wt1
423Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC168476 mRNA Translation: AAI68476.1
RefSeqiNP_001135625.1, NM_001142153.1
UniGeneiStr.44768

Genome annotation databases

GeneIDi100216184
KEGGixtr:100216184

Keywords - Coding sequence diversityi

RNA editing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC168476 mRNA Translation: AAI68476.1
RefSeqiNP_001135625.1, NM_001142153.1
UniGeneiStr.44768

3D structure databases

SMRiB5DE03
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB5DE03

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100216184
KEGGixtr:100216184

Organism-specific databases

CTDi7490
XenbaseiXB-GENE-484999 wt1

Phylogenomic databases

HOGENOMiHOG000230937
HOVERGENiHBG006960
InParanoidiB5DE03
KOiK09234

Family and domain databases

InterProiView protein in InterPro
IPR017987 Wilms_tumour
IPR000976 Wilms_tumour_N
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44601 PTHR44601, 1 hit
PfamiView protein in Pfam
PF02165 WT1, 1 hit
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 4 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiWT1_XENTR
AccessioniPrimary (citable) accession number: B5DE03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: October 14, 2008
Last modified: October 10, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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