UniProtKB - B5AR80 (OXLA_BOTPA)
Protein
L-amino-acid oxidase
Gene
N/A
Organism
Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis)
Status
Functioni
Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (highly against L-Met, L-Leu, L-Phe and L-Ile), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as antibacterial on both Gram-positive and Gram-negative bacteria and antiparasitic activities, as well as induction of platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. This protein may also have activities in hemorrhage, hemolysis, edema, and apoptosis.1 Publication
Miscellaneous
Has parasiticidal activities against leishmania, as a result of enzyme-catalyzed hydrogen peroxide production.1 Publication
Catalytic activityi
- EC:1.4.3.2
Cofactori
FADBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 89 | FADBy similarity | 1 | |
Binding sitei | 108 | SubstrateBy similarity | 1 | |
Binding sitei | 241 | SubstrateBy similarity | 1 | |
Binding sitei | 279 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 390 | SubstrateBy similarity | 1 | |
Binding sitei | 475 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 61 – 62 | FADBy similarity | 2 | |
Nucleotide bindingi | 81 – 82 | FADBy similarity | 2 | |
Nucleotide bindingi | 105 – 108 | FADBy similarity | 4 | |
Nucleotide bindingi | 482 – 487 | FADBy similarity | 6 |
GO - Molecular functioni
- L-amino-acid oxidase activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- defense response to bacterium Source: UniProtKB-KW
- hemolysis in other organism Source: UniProtKB-KW
Keywordsi
Molecular function | Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin |
Biological process | Apoptosis, Cytolysis, Hemolysis |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BRENDAi | 1.4.3.2 10579 |
Names & Taxonomyi
Protein namesi | Recommended name: L-amino-acid oxidase (EC:1.4.3.2)Short name: Bp-LAAO Short name: LAAO Short name: LAO |
Organismi | Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis) |
Taxonomic identifieri | 1042543 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothrops › |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 2 PublicationsAdd BLAST | 18 | |
ChainiPRO_0000412598 | 19 – ›503 | L-amino-acid oxidaseAdd BLAST | ›485 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 28 ↔ 191 | By similarity | ||
Glycosylationi | 190 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 349 ↔ 430 | By similarity |
Post-translational modificationi
N-glycosylated (Probable). The enzymatic activity is not affected by deglycosylation.Curated1 Publication
Keywords - PTMi
Disulfide bond, GlycoproteinExpressioni
Tissue specificityi
Expressed by the venom gland.
Interactioni
Subunit structurei
Homodimer; non-covalently linked.1 Publication
Structurei
3D structure databases
ProteinModelPortali | B5AR80 |
SMRi | B5AR80 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 482 – 483 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
HOVERGENi | HBG005729 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002937 Amino_oxidase IPR036188 FAD/NAD-bd_sf IPR001613 Flavin_amine_oxidase |
Pfami | View protein in Pfam PF01593 Amino_oxidase, 1 hit |
PRINTSi | PR00757 AMINEOXDASEF |
SUPFAMi | SSF51905 SSF51905, 1 hit |
i Sequence
Sequence statusi: Fragment.
: The displayed sequence is further processed into a mature form. Sequence processingi
B5AR80-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNVFFMFSLL FLAALGSCAD DGNPLEECFR ETDYEEFLEI AKNGLSATSN
60 70 80 90 100
PKHVVIVGAG MSGLSAAYVL ANAGHQVTVL EASKRAGGRV RTYRNDKEGW
110 120 130 140 150
YANLGPMRLP EKHRIVREYI RKFGLQLNEF SQENENAWYF IKNIRKRVGE
160 170 180 190 200
VNKDPGVLEY PVKPSEVGKS AGQLYEESLQ KAVEELRRTN CSYMLNKYDT
210 220 230 240 250
YSTKEYLLKE GNLSPGAVDM IGDLLNEDSG YYVSFIESLK HDDIFAYEKR
260 270 280 290 300
FDEIVGGMDK LPTSMYQAIQ EKVRLNVRVI KIQQDVKEVT VTYQTSAKET
310 320 330 340 350
LSVTADYVIV CTTSRAARRI KFEPPLPPKK AHALRSVHYR SGTKIFLTCT
360 370 380 390 400
KKFWEDDGIH GGKSTTDLPS RFIYYPNHNF PSGVGVIIAY GIGDDANFFQ
410 420 430 440 450
ALDFKDCGDI VINDLSLIHQ LPKEEIQAFC RPSMIQRWSL DKYAMGGITT
460 470 480 490 500
FTPYQFQHFS EALTAPVDRI YFAGEYTAQA HGWIDSTIKS GLTAARDVNR
ASE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 503 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU870608 mRNA Translation: ACG55578.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU870608 mRNA Translation: ACG55578.1 |
3D structure databases
ProteinModelPortali | B5AR80 |
SMRi | B5AR80 |
ModBasei | Search... |
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Phylogenomic databases
HOVERGENi | HBG005729 |
Enzyme and pathway databases
BRENDAi | 1.4.3.2 10579 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR002937 Amino_oxidase IPR036188 FAD/NAD-bd_sf IPR001613 Flavin_amine_oxidase |
Pfami | View protein in Pfam PF01593 Amino_oxidase, 1 hit |
PRINTSi | PR00757 AMINEOXDASEF |
SUPFAMi | SSF51905 SSF51905, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | OXLA_BOTPA | |
Accessioni | B5AR80Primary (citable) accession number: B5AR80 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 21, 2011 |
Last sequence update: | September 23, 2008 | |
Last modified: | December 5, 2018 | |
This is version 38 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families