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Protein

Beta-galactosidase

Gene

lacZ

Organism
Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103SubstrateUniRule annotation1
Metal bindingi201SodiumUniRule annotation1
Binding sitei201SubstrateUniRule annotation1
Sitei356Transition state stabilizerUniRule annotation1
Sitei390Transition state stabilizerUniRule annotation1
Metal bindingi415Magnesium 1UniRule annotation1
Metal bindingi417Magnesium 1UniRule annotation1
Active sitei460Proton donorUniRule annotation1
Metal bindingi460Magnesium 1UniRule annotation1
Binding sitei460SubstrateUniRule annotation1
Active sitei536NucleophileUniRule annotation1
Metal bindingi596Magnesium 2UniRule annotation1
Metal bindingi600Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi603SodiumUniRule annotation1
Binding sitei603SubstrateUniRule annotation1
Binding sitei1016SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMagnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:MADE_1001350
OrganismiAlteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Taxonomic identifieri1774373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
Proteomesi
  • UP000001870 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669801 – 1041Beta-galactosidaseAdd BLAST1041

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB4S2K9
SMRiB4S2K9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni536 – 539Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
KOiK01190
OMAiPSNWQLQ

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
HAMAPiMF_01687 Beta_gal, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR023933 Glyco_hydro_2_beta_Galsidase
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PANTHERiPTHR10066:SF76 PTHR10066:SF76, 1 hit
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

B4S2K9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTVAQIVAQ NDWQNPVVFQ RNRVGGHSPH HGYKTLEDAL HNANAQKYLL
60 70 80 90 100
NGEWDFCLFD APEQVPESLL AATLSAEERA KWQSIVVPSN WQLKGYDKPI
110 120 130 140 150
YCNVKYPFPV NPPIVPSENP TGCYRTTFSV TSAQLSQRNH IVFEGVNSAF
160 170 180 190 200
HLWCNGEYVG YSQDSRLPAE FDISNLLVEG ENRLAAMVIR WSDGSYLEDQ
210 220 230 240 250
DMWWLSGIFR DVSLITKPRQ HIQDVFVTPT LDACYRDATV SVRTSIKAQL
260 270 280 290 300
RCKVGIQLFD GDTTVTEQIV TGTNNKRVDE KGGWDDVVFQ TLEVKEPKHW
310 320 330 340 350
TAETPYLYRI VVSLIDDSGN VIDREAYNVG FRNVEMKNGQ LLVNGKAVLI
360 370 380 390 400
RGVNRHEHHQ VKGHAINEDD MLEDIKLLKQ NNFNAVRTAH YPNHPRWYEL
410 420 430 440 450
CDEYGLYVVD EANIETHGMF PMGRLSRDPL WAGAYMARFT QMVERDKNHP
460 470 480 490 500
SIIIWSLGNE CGHGPTHDAM YGWAKSFDPS RPVQYEGGGA DTTATDIIAP
510 520 530 540 550
MYARVDTDVE DDAVPKWAIK KWLSLPGENR PVILCEYAHA MGNSLGSFDE
560 570 580 590 600
YWKAFKDYPR LQGGFIWDWV DQGLTKHTDS GDAFWAYGGD FGDTDNDRQF
610 620 630 640 650
CINGLLFPDR TPHPHLFEAK YCQQHLSFSL TEETDKWQLS VKSDYLFRHT
660 670 680 690 700
DNELLRWQVL ENGKPIIEGE CPIYVAPQQA QTVSIAPEIN FKAGALYHLN
710 720 730 740 750
IDVVLANDCA WAKAGHVIDT AQLALANKSG LIPFVSTANV ANENAESGVT
760 770 780 790 800
VKAENTTLLV SVKNNVFSFN SESGLLTSWL HEDSEMLSAP LEDNFFRAPL
810 820 830 840 850
DNDIGVSEVD NPDPNAWESR WRRAGIGKWD RICTSVDVEQ STFDVRITSL
860 870 880 890 900
FEYHYNDKLI AATKWVYTIN HQAALTVEVE VLLDDSLPPM PRIGLQAAVP
910 920 930 940 950
APRSNEQERM RVTWQGLGPF ENYPDRKAAA RFGEHSLSIA DLQTHYIFPT
960 970 980 990 1000
DNGLRSDCKQ LNISGLRVNG QFCFSVSEYG QVQLDTAKHT SDLMPQDCVF
1010 1020 1030 1040
VYIDHAHMGV GGDDSWSPST HKAFLIEEKC YRYSVTFCAK V
Length:1,041
Mass (Da):117,714
Last modified:September 23, 2008 - v1
Checksum:i33F86621BAB56C1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA Translation: AEA96419.1
RefSeqiWP_012516793.1, NC_011138.3

Genome annotation databases

EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350
KEGGiamc:MADE_1001350
PATRICifig|314275.5.peg.278

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA Translation: AEA96419.1
RefSeqiWP_012516793.1, NC_011138.3

3D structure databases

ProteinModelPortaliB4S2K9
SMRiB4S2K9
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEA96419; AEA96419; MADE_1001350
KEGGiamc:MADE_1001350
PATRICifig|314275.5.peg.278

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
KOiK01190
OMAiPSNWQLQ

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
HAMAPiMF_01687 Beta_gal, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR023933 Glyco_hydro_2_beta_Galsidase
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PANTHERiPTHR10066:SF76 PTHR10066:SF76, 1 hit
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_ALTMD
AccessioniPrimary (citable) accession number: B4S2K9
Secondary accession number(s): F2G3U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: October 10, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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