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Protein

DIMBOA UDP-glucosyltransferase BX9

Gene

BX9

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glucosyltransferase involved in the last step of benzoxazinoid glucoside biosynthesis. Catalyzes the glucosylation of hydroxamic acids utilizing UDP-glucose as glucose doner, reducing the toxicity of these natural insecticides for storage. Can use DIMBOA and DIBOA as substrates, HMBOA (2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) and HBOA (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) with a lower efficiency, but not indole acetic acid or quercitin.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside.2 Publications
UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside.2 Publications

Cofactori

Mg2+1 Publication, Ca2+1 Publication

Kineticsi

kcat is 11.6 sec(-1) for DIMBOA. kcat is 12.5 sec(-1) for DIBOA. kcat is 22.6 sec(-1) for UDPG.
  1. KM=71 µM for 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA)2 Publications
  2. KM=1300 µM for 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA)2 Publications
  3. KM=96 µM for uridine 5'-diphosphoglucose (UDPG)2 Publications

    pH dependencei

    Optimum pH is 8.5.2 Publications

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei278UDP-glucoseBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10603
    SABIO-RKiB4G072

    Protein family/group databases

    CAZyiGT1 Glycosyltransferase Family 1

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DIMBOA UDP-glucosyltransferase BX9 (EC:2.4.1.2022 Publications)
    Alternative name(s):
    2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase BX9
    Protein BENZOXAZINLESS 9
    Gene namesi
    Name:BX9
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
    Proteomesi
    • UP000007305 Componenti: Chromosome 1

    Organism-specific databases

    MaizeGDBi9021865

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004153071 – 462DIMBOA UDP-glucosyltransferase BX9Add BLAST462

    Proteomic databases

    PaxDbiB4G072
    PRIDEiB4G072

    Expressioni

    Tissue specificityi

    Expressed at the same levels in roots and shoots.1 Publication

    Developmental stagei

    Highly expressed in young seedlings up to 4 days after imbibition.1 Publication

    Gene expression databases

    ExpressionAtlasiB4G072 differential
    GenevisibleiB4G072 ZM

    Interactioni

    Protein-protein interaction databases

    STRINGi4577.GRMZM2G161335_P01

    Structurei

    3D structure databases

    SMRiB4G072
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni336 – 338UDP-glucose bindingBy similarity3
    Regioni353 – 361UDP-glucose bindingBy similarity9
    Regioni375 – 378UDP-glucose bindingBy similarity4

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiKOG1192 Eukaryota
    COG1819 LUCA
    HOGENOMiHOG000237564
    KOiK13227
    OMAiIHEDMSI
    OrthoDBiEOG09360BKY

    Family and domain databases

    InterProiView protein in InterPro
    IPR002213 UDP_glucos_trans
    PfamiView protein in Pfam
    PF00201 UDPGT, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

    B4G072-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MASSRTGAGA GGRVVVFPFP FQGHFNPVMR LARALHARGL AITVFHSGAL
    60 70 80 90 100
    DPADYPADYR FVPVTVEADP KLLASEDIAA IVTTLNASCD APFRARLSAL
    110 120 130 140 150
    LAAEGRDSVR CVFTDVSWNA VLTASSDLGV PALGMMTASA ASLRDYMAYR
    160 170 180 190 200
    TLIDKGYLPV KEERKEDPVP ELPPYLVKDL LRVDTSDLEE FAELLARTVT
    210 220 230 240 250
    AARRASGLIF NTFPLIETDT LAEIHKALSV PVFAVAPLNK LVPTATASLH
    260 270 280 290 300
    GVVQADRGCL QWLDTQQPGS VLYVSFGSMA AMDPHEFVEL AWGLADSKRP
    310 320 330 340 350
    FVWVVRPNLI RGFESGALPD GVEDEVRGRG IVVAWAPQEE VLAHPAVGGF
    360 370 380 390 400
    LTHNGWNSTV EAISEGVPMV CCPRHGDQFG NMRYVCDVWK VGTELVGEQL
    410 420 430 440 450
    ERGQVKAAID RLFGTKEGEE IKERMKEFKI AAAKGIGIGV DVDETASPRT
    460
    DLTDLVDLIK SF
    Length:462
    Mass (Da):50,017
    Last modified:September 23, 2008 - v1
    Checksum:i2314E86FA7AF9A6F
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    A0A1D6KGW5A0A1D6KGW5_MAIZE
    Benzoxazinone synthesis9
    100274317 ZEAMMB73_Zm00001d031209
    185Annotation score:
    A0A1R3M158A0A1R3M158_MAIZE
    Benzoxazinone synthesis9
    ZEAMMB73_Zm00001d031209
    462Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti6T → TGA in AAL57038 (PubMed:11851909).Curated1
    Sequence conflicti176L → R in AAL57038 (PubMed:11851909).Curated1
    Sequence conflicti334A → T in AAL57038 (PubMed:11851909).Curated1
    Sequence conflicti446A → T in AAL57038 (PubMed:11851909).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF331855 Genomic DNA Translation: AAL57038.1
    BT042760 mRNA Translation: ACF87765.1
    RefSeqiNP_001142152.1, NM_001148680.1
    UniGeneiZm.67985

    Genome annotation databases

    EnsemblPlantsiZm00001d031209_T001; Zm00001d031209_P001; Zm00001d031209
    GeneIDi100274317
    GrameneiZm00001d031209_T001; Zm00001d031209_P001; Zm00001d031209
    KEGGizma:100274317

    Similar proteinsi

    Entry informationi

    Entry nameiBX9_MAIZE
    AccessioniPrimary (citable) accession number: B4G072
    Secondary accession number(s): Q8W2B6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: September 23, 2008
    Last modified: May 23, 2018
    This is version 61 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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