UniProtKB - B4F769 (SMAL1_RAT)
Protein
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
Gene
Smarcal1
Organism
Rattus norvegicus (Rat)
Status
Functioni
ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 415 – 422 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- annealing helicase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- DNA-dependent ATPase activity Source: UniProtKB
- DNA helicase activity Source: GO_Central
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA metabolic process Source: UniProtKB
- DNA repair Source: GO_Central
- DNA rewinding Source: GO_Central
- double-strand break repair via nonhomologous end joining Source: RGD
- regulation of transcription by RNA polymerase II Source: UniProtKB
- replication fork processing Source: UniProtKB
- replication fork protection Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)Alternative name(s): HepA-related protein Sucrose nonfermenting protein 2-like 1 |
Gene namesi | Name:Smarcal1 Synonyms:Harp |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1306134, Smarcal1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Recruited to damaged DNA regions.By similarity
Nucleus
- DNA replication factor A complex Source: Ensembl
- nuclear replication fork Source: GO_Central
- nucleus Source: UniProtKB
Other locations
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000361532 | 2 – 910 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1Add BLAST | 909 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 117 | PhosphoserineBy similarity | 1 | |
Modified residuei | 164 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | B4F769 |
PeptideAtlasi | B4F769 |
PRIDEi | B4F769 |
PTM databases
PhosphoSitePlusi | B4F769 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000016503, Expressed in testis and 22 other tissues |
Genevisiblei | B4F769, RN |
Interactioni
Subunit structurei
Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.
By similarityProtein-protein interaction databases
BioGRIDi | 261356, 1 interactor |
STRINGi | 10116.ENSRNOP00000022459 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 268 | HARP 1PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 284 – 355 | HARP 2PROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 402 – 557 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 156 | |
Domaini | 672 – 825 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 154 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 30 | Mediates interaction with RPA2By similarityAdd BLAST | 29 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 3 – 28 | Sequence analysisAdd BLAST | 26 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 506 – 509 | DESH box | 4 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG1000, Eukaryota |
GeneTreei | ENSGT00940000157608 |
HOGENOMi | CLU_000315_33_1_1 |
InParanoidi | B4F769 |
OMAi | SIMEMFQ |
OrthoDBi | 1082831at2759 |
PhylomeDBi | B4F769 |
TreeFami | TF106474 |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR030101, HARP(SMARCAL1) IPR010003, HARP_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
PANTHERi | PTHR45766:SF3, PTHR45766:SF3, 2 hits |
Pfami | View protein in Pfam PF07443, HARP, 2 hits PF00271, Helicase_C, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51467, HARP, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
B4F769-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLPLTEEQR KKIEENRQKA LARRAEKLWA EQPQSTASGS SAARPSQCKQ
60 70 80 90 100
NSLLNLPAEP SKPEGHATIS KGQNLNNSLP AAQRPHSSPC FQPSTAEEAK
110 120 130 140 150
GLWKSEGKMS AACPNPSPPE VSNQQLLGSK SSEGHPQATQ DTAASCPRPF
160 170 180 190 200
PRDPKLEAKA GRPSTSGQSI SDTFYALGEK TPKTDGRPAK ALQTSPQKAS
210 220 230 240 250
CLRGMCLRTG DRFRVKIGYN KELIEVFKSL PSRRYDSFTK TWDFSMSDYR
260 270 280 290 300
ALMKAVERLS TVSLQPLEEV DGTGGQTSLP SAPSLTFVTG RCMLISRARF
310 320 330 340 350
EVDIGYSEVV IALFKQMESR NYDPKTRKWN FLLEEHNKLI ARSRELKQVQ
360 370 380 390 400
LDPLPKTLTL AFASQLEKTS LQSKADVPEA DLSGVDAKLV SNLMPFQREG
410 420 430 440 450
VSFAISKRGR LLLADDMGLG KTIQAICIAA FYRKEWPLLV VVPSSVRFTW
460 470 480 490 500
EQAFLRWLPS LSPEDINVVV TGKGRLTAGL VNIVSFDLLS KLEKQLKTPF
510 520 530 540 550
KVVIIDESHF LKNIKTARCR AAVPILKVAK RVILLSGTPA MSRPAELYTQ
560 570 580 590 600
IIAVKPTFFP QFHAFGLRYC DAKRLPWGWD YSGSSNLGEL KLLLEEAVML
610 620 630 640 650
RRLKSDVLSQ LPAKQRKMVV VNPGRISTRA KAALDAAAKE MTKDKTKQQQ
660 670 680 690 700
KEALLVFFNR TAEAKIPCVI EYILDLLESG REKFLVFAHH KVLLDAIAKE
710 720 730 740 750
LERKNVQHIR IDGSTPSADR EDLCQQFQLS KGHTVAVLSI TAANMGLTFS
760 770 780 790 800
SADLVVFAEL FWNPGVLIQA EDRVHRIGQT NSVGIHYLVA KGTADDYLWP
810 820 830 840 850
LIQEKIKVLG EAGLSETNFS EMTEATDYLY KDPKQKTIYS LFQQSFEDDG
860 870 880 890 900
NDMEFLEAAE SFELGSTSGT SGNSSQELGD ITDENALADS PPKKRRFEFF
910
DNWDSFTSPF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474004 Genomic DNA Translation: EDL75309.1 BC168154 mRNA Translation: AAI68154.1 |
RefSeqi | NP_001101692.1, NM_001108222.1 XP_006245274.1, XM_006245212.3 XP_006245275.1, XM_006245213.3 XP_008765457.1, XM_008767235.1 XP_017451963.1, XM_017596474.1 |
Genome annotation databases
Ensembli | ENSRNOT00000022459; ENSRNOP00000022459; ENSRNOG00000016503 ENSRNOT00000071856; ENSRNOP00000064323; ENSRNOG00000016503 |
GeneIDi | 316477 |
KEGGi | rno:316477 |
UCSCi | RGD:1306134, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474004 Genomic DNA Translation: EDL75309.1 BC168154 mRNA Translation: AAI68154.1 |
RefSeqi | NP_001101692.1, NM_001108222.1 XP_006245274.1, XM_006245212.3 XP_006245275.1, XM_006245213.3 XP_008765457.1, XM_008767235.1 XP_017451963.1, XM_017596474.1 |
3D structure databases
SMRi | B4F769 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 261356, 1 interactor |
STRINGi | 10116.ENSRNOP00000022459 |
PTM databases
PhosphoSitePlusi | B4F769 |
Proteomic databases
PaxDbi | B4F769 |
PeptideAtlasi | B4F769 |
PRIDEi | B4F769 |
Genome annotation databases
Ensembli | ENSRNOT00000022459; ENSRNOP00000022459; ENSRNOG00000016503 ENSRNOT00000071856; ENSRNOP00000064323; ENSRNOG00000016503 |
GeneIDi | 316477 |
KEGGi | rno:316477 |
UCSCi | RGD:1306134, rat |
Organism-specific databases
CTDi | 50485 |
RGDi | 1306134, Smarcal1 |
Phylogenomic databases
eggNOGi | KOG1000, Eukaryota |
GeneTreei | ENSGT00940000157608 |
HOGENOMi | CLU_000315_33_1_1 |
InParanoidi | B4F769 |
OMAi | SIMEMFQ |
OrthoDBi | 1082831at2759 |
PhylomeDBi | B4F769 |
TreeFami | TF106474 |
Miscellaneous databases
PROi | PR:B4F769 |
Gene expression databases
Bgeei | ENSRNOG00000016503, Expressed in testis and 22 other tissues |
Genevisiblei | B4F769, RN |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR030101, HARP(SMARCAL1) IPR010003, HARP_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
PANTHERi | PTHR45766:SF3, PTHR45766:SF3, 2 hits |
Pfami | View protein in Pfam PF07443, HARP, 2 hits PF00271, Helicase_C, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51467, HARP, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SMAL1_RAT | |
Accessioni | B4F769Primary (citable) accession number: B4F769 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | September 23, 2008 | |
Last modified: | December 2, 2020 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families