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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

Gene

Smarcal1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi415 – 422ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)
Alternative name(s):
HepA-related protein
Sucrose nonfermenting protein 2-like 1
Gene namesi
Name:Smarcal1
Synonyms:Harp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1306134 Smarcal1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003615322 – 910SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1Add BLAST909

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei117PhosphoserineBy similarity1
Modified residuei164PhosphoserineBy similarity1

Post-translational modificationi

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiB4F769
PeptideAtlasiB4F769
PRIDEiB4F769

PTM databases

PhosphoSitePlusiB4F769

Expressioni

Gene expression databases

BgeeiENSRNOG00000016503
GenevisibleiB4F769 RN

Interactioni

Subunit structurei

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064323

Structurei

3D structure databases

SMRiB4F769
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 268HARP 1PROSITE-ProRule annotationAdd BLAST71
Domaini284 – 355HARP 2PROSITE-ProRule annotationAdd BLAST72
Domaini402 – 557Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST156
Domaini672 – 825Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 30Mediates interaction with RPA2By similarityAdd BLAST29

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili3 – 28Sequence analysisAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi506 – 509DESH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1000 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00910000144109
HOGENOMiHOG000018734
HOVERGENiHBG054110
InParanoidiB4F769
KOiK14440
OMAiANMGLTF
OrthoDBiEOG091G0A1B
PhylomeDBiB4F769
TreeFamiTF106474

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR030101 HARP(SMARCAL1)
IPR010003 HARP_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PANTHERiPTHR10799:SF731 PTHR10799:SF731, 2 hits
PfamiView protein in Pfam
PF07443 HARP, 2 hits
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS51467 HARP, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4F769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPLTEEQR KKIEENRQKA LARRAEKLWA EQPQSTASGS SAARPSQCKQ
60 70 80 90 100
NSLLNLPAEP SKPEGHATIS KGQNLNNSLP AAQRPHSSPC FQPSTAEEAK
110 120 130 140 150
GLWKSEGKMS AACPNPSPPE VSNQQLLGSK SSEGHPQATQ DTAASCPRPF
160 170 180 190 200
PRDPKLEAKA GRPSTSGQSI SDTFYALGEK TPKTDGRPAK ALQTSPQKAS
210 220 230 240 250
CLRGMCLRTG DRFRVKIGYN KELIEVFKSL PSRRYDSFTK TWDFSMSDYR
260 270 280 290 300
ALMKAVERLS TVSLQPLEEV DGTGGQTSLP SAPSLTFVTG RCMLISRARF
310 320 330 340 350
EVDIGYSEVV IALFKQMESR NYDPKTRKWN FLLEEHNKLI ARSRELKQVQ
360 370 380 390 400
LDPLPKTLTL AFASQLEKTS LQSKADVPEA DLSGVDAKLV SNLMPFQREG
410 420 430 440 450
VSFAISKRGR LLLADDMGLG KTIQAICIAA FYRKEWPLLV VVPSSVRFTW
460 470 480 490 500
EQAFLRWLPS LSPEDINVVV TGKGRLTAGL VNIVSFDLLS KLEKQLKTPF
510 520 530 540 550
KVVIIDESHF LKNIKTARCR AAVPILKVAK RVILLSGTPA MSRPAELYTQ
560 570 580 590 600
IIAVKPTFFP QFHAFGLRYC DAKRLPWGWD YSGSSNLGEL KLLLEEAVML
610 620 630 640 650
RRLKSDVLSQ LPAKQRKMVV VNPGRISTRA KAALDAAAKE MTKDKTKQQQ
660 670 680 690 700
KEALLVFFNR TAEAKIPCVI EYILDLLESG REKFLVFAHH KVLLDAIAKE
710 720 730 740 750
LERKNVQHIR IDGSTPSADR EDLCQQFQLS KGHTVAVLSI TAANMGLTFS
760 770 780 790 800
SADLVVFAEL FWNPGVLIQA EDRVHRIGQT NSVGIHYLVA KGTADDYLWP
810 820 830 840 850
LIQEKIKVLG EAGLSETNFS EMTEATDYLY KDPKQKTIYS LFQQSFEDDG
860 870 880 890 900
NDMEFLEAAE SFELGSTSGT SGNSSQELGD ITDENALADS PPKKRRFEFF
910
DNWDSFTSPF
Length:910
Mass (Da):101,212
Last modified:September 23, 2008 - v1
Checksum:iA683FBA1FC495F5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474004 Genomic DNA Translation: EDL75309.1
BC168154 mRNA Translation: AAI68154.1
RefSeqiNP_001101692.1, NM_001108222.1
XP_006245274.1, XM_006245212.3
XP_006245275.1, XM_006245213.3
XP_008765457.1, XM_008767235.1
XP_017451963.1, XM_017596474.1
UniGeneiRn.34679

Genome annotation databases

EnsembliENSRNOT00000022459; ENSRNOP00000022459; ENSRNOG00000016503
ENSRNOT00000071856; ENSRNOP00000064323; ENSRNOG00000016503
GeneIDi316477
KEGGirno:316477
UCSCiRGD:1306134 rat

Similar proteinsi

Entry informationi

Entry nameiSMAL1_RAT
AccessioniPrimary (citable) accession number: B4F769
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: September 23, 2008
Last modified: April 25, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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