UniProtKB - B4DUQ9 (B4DUQ9_HUMAN)
DNA replication licensing factor MCM3
Functioni
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
UniRule annotationCatalytic activityi
- EC:3.6.4.12UniRule annotationARBA annotation
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ATP hydrolysis activity Source: RHEA
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- DNA replication initiation Source: InterPro
Keywordsi
Molecular function | DNA-bindingUniRule annotationARBA annotation, HelicaseUniRule annotationARBA annotation, Hydrolase |
Biological process | Cell cycleARBA annotation, DNA replicationUniRule annotation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM3UniRule annotation (EC:3.6.4.12UniRule annotation) |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Subcellular locationi
Nucleus
- Nucleus UniRule annotationARBA annotation
Other locations
- Chromosome ARBA annotation
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- chromosome Source: UniProtKB-SubCell
- MCM complex Source: UniProtKB-UniRule
Keywords - Cellular componenti
ChromosomeARBA annotation, NucleusUniRule annotationInteractioni
Subunit structurei
Component of the MCM2-7 complex.
UniRule annotationStructurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 249 – 455 | MCMInterPro annotationAdd BLAST | 207 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 616 – 693 | DisorderedSequence analysisAdd BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 636 – 661 | Basic and acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 668 – 693 | Basic and acidic residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR031327, MCM IPR008046, Mcm3 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01659, MCMPROTEIN3 |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MWSYGRLREI TWTSWTTRLL NNAFEELVAF QRALKDFVAS IDATYAKQYE
60 70 80 90 100
EFYVGLEGSF GSKHVSPRTL TSCFLSCVVC VEGIVTKCSL VRPKVVRSVH
110 120 130 140 150
YCPATKKTIE RRYSDLTTLV AFPSSSVYPT KDEENNPLET EYGLSVYKDH
160 170 180 190 200
QTITIQEMPE KAPAGQLPRS VDVILDDDLV DKAKPGDRVQ VVGTYRCLPG
210 220 230 240 250
KKGGYTSGTF RTVLIACNVK QMSKDAQPSF SAEDIAKIKK FSKTRSKDIF
260 270 280 290 300
DQLAKSLAPS IHGHDYVKKA ILCLLLGGVE RDLENGSHIR GDINILLIGD
310 320 330 340 350
PSVAKSQLLR YVLCTAPRAI PTTGRGSSGV GLTAAVTTDQ ETGERRLEAG
360 370 380 390 400
AMVLADRGVV CIDEFDKMSD MDRTAIHEVM EQGRVTIAKA GIHARLNARC
410 420 430 440 450
SVLAAANPVY GRYDQYKTPM ENIGLQDSLL SRFDLLFIML DQMDPEQDRE
460 470 480 490 500
ISDHVLRMHR YRAPGEQDGD AMPLGSAVDI LATDDPNFSQ EDQQDTQIYE
510 520 530 540 550
KHDNLLHGTK KKKEKMVGAA FMKKYIHVAK IIKPVLTQES ATYIAEEYSR
560 570 580 590 600
LRSQDSMSSD TARTSPVTAR TLETLIRLAT AHAKARMSKT VDLQDAEEAV
610 620 630 640 650
ELVQYAYFKK VLEKEKKRKK RSEDESETED EEEKSQEDQE QKRKRRKTRQ
660 670 680 690 700
PDAKDGDSYD PYDFSDTEEE MPQVHTPKTA DSQETKESQK VELSESRLKA
710 720 730 740 750
FKVALLDVFR EAHAQSIGMN RLTESINRDS EEPFSSVEIQ AALSKMQDDN
760
QVMVSEGIIF LI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK300754 mRNA Translation: BAG62421.1 |
RefSeqi | NP_001257401.1, NM_001270472.1 |
Genome annotation databases
GeneIDi | 4172 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK300754 mRNA Translation: BAG62421.1 |
RefSeqi | NP_001257401.1, NM_001270472.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
MaxQBi | B4DUQ9 |
PeptideAtlasi | B4DUQ9 |
Protocols and materials databases
DNASUi | 4172 |
Genome annotation databases
GeneIDi | 4172 |
Organism-specific databases
CTDi | 4172 |
Miscellaneous databases
BioGRID-ORCSi | 4172, 722 hits in 1067 CRISPR screens |
GenomeRNAii | 4172 |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR031327, MCM IPR008046, Mcm3 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01659, MCMPROTEIN3 |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | B4DUQ9_HUMAN | |
Accessioni | B4DUQ9Primary (citable) accession number: B4DUQ9 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | September 23, 2008 |
Last sequence update: | September 23, 2008 | |
Last modified: | May 25, 2022 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |