UniProtKB - B3VK56 (B3VK56_HUMAN)
Protein
Nitric oxide synthase
Gene
NOS1
Organism
Homo sapiens (Human)
Status
Functioni
Produces nitric oxide (NO).UniRule annotation
Catalytic activityi
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxideUniRule annotationARBA annotationEC:1.14.13.39UniRule annotationARBA annotation
Cofactori
Protein has several cofactor binding sites:- (6R)-L-erythro-5,6,7,8-tetrahydrobiopterinARBA annotation
- hemeUniRule annotationARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 420 | Iron (heme axial ligand)UniRule annotation | 1 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: InterPro
- FMN binding Source: InterPro
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- nitric-oxide synthase activity Source: UniProtKB-EC
GO - Biological processi
- nitric oxide biosynthetic process Source: InterPro
Keywordsi
Molecular function | Calmodulin-bindingUniRule annotationARBA annotation, OxidoreductaseUniRule annotationARBA annotation |
Ligand | FADUniRule annotationARBA annotation, Flavoprotein, FMNUniRule annotationARBA annotation, HemeUniRule annotationARBA annotation, Iron, Metal-binding, NADPUniRule annotationARBA annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NOS1Imported ORF Names:hCG_40354Imported |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Organism-specific databases
VEuPathDBi | HostDB:ENSG00000089250.18 |
Subcellular locationi
Plasma membrane
- sarcolemma ARBA annotation; Peripheral membrane protein ARBA annotation
Other locations
- dendritic spine ARBA annotation
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: HPA
- sarcolemma Source: UniProtKB-SubCell
Other locations
- dendritic spine Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projectionARBA annotationExpressioni
Gene expression databases
Bgeei | ENSG00000089250, Expressed in vastus lateralis and 134 other tissues |
ExpressionAtlasi | B3VK56, baseline and differential |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Chemistry databases
BindingDBi | B3VK56 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 99 | PDZInterPro annotationAdd BLAST | 83 | |
Domaini | 760 – 940 | Flavodoxin-likeInterPro annotationAdd BLAST | 181 | |
Domaini | 995 – 1242 | FAD-binding FR-typeInterPro annotationAdd BLAST | 248 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 112 – 192 | DisorderedSequence analysisAdd BLAST | 81 | |
Regioni | 276 – 302 | DisorderedSequence analysisAdd BLAST | 27 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 280 – 299 | PolarSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_001570_16_0_1 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 2.30.42.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR001478, PDZ IPR036034, PDZ_sf IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit PF00595, PDZ, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit PS50106, PDZ, 1 hit |
i Sequence
Sequence statusi: Complete.
B3VK56-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDHMFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG
60 70 80 90 100
AAEQSGLIQA GDIILAVNGR PLVDLSYDSA LEVLRGIASE THVVLILRGP
110 120 130 140 150
EGFTTHLETT FTGDGTPKTI RVTQPLGPPT KAVDLSHQPP AGKEQPLAVD
160 170 180 190 200
GASGPGNGPQ HAYDDGQEAG SLPHANGLAP RPPGQDPAKK ATRVSLQGRG
210 220 230 240 250
ENNELLKEIE PVLSLLTSGS RGVKGGAPAK AEMKDMGIQV DRDLDGKSHK
260 270 280 290 300
PLPLGVENDR VFNDLWGKGN VPVVLNNPYS EKEQPPTSGK QSPTKNGSPS
310 320 330 340 350
KCPRFLKVKN WETEVVLTDT LHLKSTLETG CTEYICMGSI MHPSQHARRP
360 370 380 390 400
EDVRTKGQLF PLAKEFIDQY YSSIKRFGSK AHMERLEEVN KEIDTTSTYQ
410 420 430 440 450
LKDTELIYGA KHAWRNASRC VGRIQWSKLQ VFDARDCTTA HGMFNYICNH
460 470 480 490 500
VKYATNKGNL RSAITIFPQR TDGKHDFRVW NSQLIRYAGY KQPDGSTLGD
510 520 530 540 550
PANVQFTEIC IQQGWKPPRG RFDVLPLLLQ ANGNDPELFQ IPPELVLEVP
560 570 580 590 600
IRHPKFEWFK DLGLKWYGLP AVSNMLLEIG GLEFSACPFS GWYMGTEIGV
610 620 630 640 650
RDYCDNSRYN ILEEVAKKMN LDMRKTSSLW KDQALVEINI AVLYSFQSDK
660 670 680 690 700
VTIVDHHSAT ESFIKHMENE YRCRGGCPAD WVWIVPPMSG SITPVFHQEM
710 720 730 740 750
LNYRLTPSFE YQPDPWNTHV WKGTNGTPTK RRAIGFKKLA EAVKFSAKLM
760 770 780 790 800
GQAMAKRVKA TILYATETGK SQAYAKTLCE IFKHAFDAKV MSMEEYDIVH
810 820 830 840 850
LEHETLVLVV TSTFGNGDPP ENGEKFGCAL MEMRHPNSVQ EERKSYKVRF
860 870 880 890 900
NSVSSYSDSQ KSSGDGPDLR DNFESAGPLA NVRFSVFGLG SRAYPHFCAF
910 920 930 940 950
GHAVDTLLEE LGGERILKMR EGDELCGQEE AFRTWAKKVF KAACDVFCVG
960 970 980 990 1000
DDVNIEKANN SLISNDRSWK RNKFRLTFVA EAPELTQGLS NVHKKRVSAA
1010 1020 1030 1040 1050
RLLSRQNLQS PKSSRSTIFV RLHTNGSQEL QYQPGDHLGV FPGNHEDLVN
1060 1070 1080 1090 1100
ALIERLEDAP PVNQMVKVEL LEERNTALGV ISNWTDELRL PPCTIFQAFK
1110 1120 1130 1140 1150
YYLDITTPPT PLQLQQFASL ATSEKEKQRL LVLSKGLQEY EEWKWGKNPT
1160 1170 1180 1190 1200
IVEVLEEFPS IQMPATLLLT QLSLLQPRYY SISSSPDMYP DEVHLTVAIV
1210 1220 1230 1240 1250
SYRTRDGEGP IHHGVCSSWL NRIQADELVP CFVRGAPSFH LPRNPQVPCI
1260 1270 1280 1290 1300
LVGPGTGIAP FRSFWQQRQF DIQHKGMNPC PMVLVFGCRQ SKIDHIYREE
1310 1320 1330 1340 1350
TLQAKNKGVF RELYTAYSRE PDKPKKYVQD ILQEQLAESV YRALKEQGGH
1360 1370 1380 1390 1400
IYVCGDVTMA ADVLKAIQRI MTQQGKLSAE DAGVFISRMR DDNRYHEDIF
1410 1420 1430
GVTLRTYEVT NRLRSESIAF IEESKKDTDE VFSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU753241 mRNA Translation: ACF04132.1 CH471054 Genomic DNA Translation: EAW98110.1 |
RefSeqi | NP_000611.1, NM_000620.4 XP_016874836.1, XM_017019347.1 |
Genome annotation databases
GeneIDi | 4842 |
KEGGi | hsa:4842 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU753241 mRNA Translation: ACF04132.1 CH471054 Genomic DNA Translation: EAW98110.1 |
RefSeqi | NP_000611.1, NM_000620.4 XP_016874836.1, XM_017019347.1 |
3D structure databases
SMRi | B3VK56 |
ModBasei | Search... |
Chemistry databases
BindingDBi | B3VK56 |
Protocols and materials databases
Antibodypediai | 3691, 908 antibodies |
DNASUi | 4842 |
Genome annotation databases
GeneIDi | 4842 |
KEGGi | hsa:4842 |
Organism-specific databases
CTDi | 4842 |
PharmGKBi | PA252 |
VEuPathDBi | HostDB:ENSG00000089250.18 |
Phylogenomic databases
HOGENOMi | CLU_001570_16_0_1 |
Miscellaneous databases
BioGRID-ORCSi | 4842, 4 hits in 875 CRISPR screens |
ChiTaRSi | NOS1, human |
GenomeRNAii | 4842 |
Gene expression databases
Bgeei | ENSG00000089250, Expressed in vastus lateralis and 134 other tissues |
ExpressionAtlasi | B3VK56, baseline and differential |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 2.30.42.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR001478, PDZ IPR036034, PDZ_sf IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit PF00595, PDZ, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | B3VK56_HUMAN | |
Accessioni | B3VK56Primary (citable) accession number: B3VK56 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | September 2, 2008 |
Last sequence update: | September 2, 2008 | |
Last modified: | February 10, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |