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UniProtKB - B3PDE5 (CE2B_CELJU)
Protein
Acetylxylan esterase / glucomannan deacetylase
Gene
ce2C
Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Functioni
Involved in the degradation of plant cell wall polysaccharides. Catalyzes the deacetylation of acetylated birchwood xylan and glucomannan, with a large preference for the latter, and of the synthetic substrate 4-nitrophenyl acetate (4-NPAc).
1 PublicationCatalytic activityi
- Deacetylation of xylans and xylo-oligosaccharides.1 Publication EC:3.1.1.72
Kineticsi
kcat is 49820 min(-1) for the deacetylation of 4-nitrophenyl acetate. kcat is 66 min(-1) for the deacetylation of birchwood xylan. kcat is 1348 min(-1) for the deacetylation of glucomannan.1 Publication
- KM=262 µM for 4-nitrophenyl acetate1 Publication
- KM=4.6 mM for acetylated birchwood xylan1 Publication
- KM=0.84 mM for acetylated glucomannan1 Publication
: xylan degradation Pathwayi
This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.1 PublicationView all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 151 | Nucleophile1 Publication | 1 | |
Sitei | 199 | Transition state stabilizerBy similarity | 1 | |
Sitei | 247 | Transition state stabilizerBy similarity | 1 | |
Sitei | 336 | Increases nucleophilicity of active site Ser1 Publication | 1 |
GO - Molecular functioni
- acetylxylan esterase activity Source: UniProtKB
- hydrolase activity, acting on glycosyl bonds Source: UniProtKB-KW
GO - Biological processi
- glucomannan catabolic process Source: UniProtKB
- xylan catabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Polysaccharide degradation |
Enzyme and pathway databases
UniPathwayi | UPA00114 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ce2CImported Ordered Locus Names:CJA_3103Imported |
Organismi | Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
Taxonomic identifieri | 498211 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Cellvibrionales › Cellvibrionaceae › Cellvibrio › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000434124 | 22 – 360 | Acetylxylan esterase / glucomannan deacetylaseAdd BLAST | 339 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | B3PDE5 |
SMRi | B3PDE5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | B3PDE5 |
Family & Domainsi
Sequence similaritiesi
Belongs to the carbohydrate esterase 2 (CE2) family.1 Publication
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG2755, Bacteria |
HOGENOMi | CLU_042506_1_0_6 |
OMAi | TWPGTGV |
OrthoDBi | 1247435at2 |
Family and domain databases
CDDi | cd01831, Endoglucanase_E_like, 1 hit |
Gene3Di | 3.40.50.1110, 1 hit |
InterProi | View protein in InterPro IPR040794, CE2_N IPR037461, CtCE2-like_dom IPR013830, SGNH_hydro IPR036514, SGNH_hydro_sf |
Pfami | View protein in Pfam PF17996, CE2_N, 1 hit PF13472, Lipase_GDSL_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
B3PDE5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPHALIGLL AGMLLSSSLY AADSTKPLPL HIGGRVLVES PANQPVSYTY
60 70 80 90 100
SWPAVYFETA FKGQSLTLKF DDDQNIFRLI VDDKAPVVIN KPGKVDYPVE
110 120 130 140 150
SLAPGKHRVR LEKLTETQST SGRFLGFYTD PSAKPLALPK RKRQIEFIGD
160 170 180 190 200
SFTVGYGNTS PSRECTDEEL FKTTNSQMAF GPLTAKAFDA DYQINASSGF
210 220 230 240 250
GIVRNYNGTS PDKSLLSLYP YTLNNPDQLY HNKHWKPQVI VIGLGTNDFS
260 270 280 290 300
TALNDNERWK TREALHADYV ANYVKFVKQL HSNNARAQFI LMNSDQSNGE
310 320 330 340 350
IAEQVGKVVA QLKGGGLHQV EQIVFKGLDY SGCHWHPSAN DDQLLANLLI
360
THLQQKKGIW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000934 Genomic DNA Translation: ACE85322.1 |
RefSeqi | WP_012488681.1, NC_010995.1 |
Genome annotation databases
EnsemblBacteriai | ACE85322; ACE85322; CJA_3103 |
KEGGi | cja:CJA_3103 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000934 Genomic DNA Translation: ACE85322.1 |
RefSeqi | WP_012488681.1, NC_010995.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2W9X | X-ray | 2.00 | A/B | 1-360 | [»] | |
AlphaFoldDBi | B3PDE5 | |||||
SMRi | B3PDE5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 498211.CJA_3103 |
Genome annotation databases
EnsemblBacteriai | ACE85322; ACE85322; CJA_3103 |
KEGGi | cja:CJA_3103 |
Phylogenomic databases
eggNOGi | COG2755, Bacteria |
HOGENOMi | CLU_042506_1_0_6 |
OMAi | TWPGTGV |
OrthoDBi | 1247435at2 |
Enzyme and pathway databases
UniPathwayi | UPA00114 |
Miscellaneous databases
EvolutionaryTracei | B3PDE5 |
Family and domain databases
CDDi | cd01831, Endoglucanase_E_like, 1 hit |
Gene3Di | 3.40.50.1110, 1 hit |
InterProi | View protein in InterPro IPR040794, CE2_N IPR037461, CtCE2-like_dom IPR013830, SGNH_hydro IPR036514, SGNH_hydro_sf |
Pfami | View protein in Pfam PF17996, CE2_N, 1 hit PF13472, Lipase_GDSL_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CE2B_CELJU | |
Accessioni | B3PDE5Primary (citable) accession number: B3PDE5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 14, 2015 |
Last sequence update: | September 2, 2008 | |
Last modified: | May 25, 2022 | |
This is version 68 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families