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Protein

PAN2-PAN3 deadenylation complex catalytic subunit PAN2

Gene

PAN2

Organism
Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 2 metal cations per subunit in the catalytic exonuclease domain.UniRule annotation

Enzyme regulationi

Positively regulated by the regulatory subunit PAN3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi910Divalent metal cation; catalyticUniRule annotation1
Metal bindingi912Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1020Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1071Divalent metal cation; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonucleaseUniRule annotation, Hydrolase, Nuclease
Biological processmRNA processingUniRule annotation
LigandMetal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
PAN2-PAN3 deadenylation complex catalytic subunit PAN2UniRule annotation (EC:3.1.13.4UniRule annotation)
Alternative name(s):
PAB1P-dependent poly(A)-specific ribonucleaseUniRule annotation
Poly(A)-nuclease deadenylation complex subunit 2UniRule annotation
Short name:
PAN deadenylation complex subunit 2UniRule annotation
Gene namesi
Name:PAN2UniRule annotation
ORF Names:SCRG_01092Imported
OrganismiSaccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)Imported
Taxonomic identifieri285006 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000008335 Componenti: Unassembled WGS sequence

Subcellular locationi

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB3LHF9
SMRiB3LHF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini505 – 855USPInterPro annotationAdd BLAST351

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni337 – 473LinkerUniRule annotationAdd BLAST137

Domaini

Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.UniRule annotation
The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. PAN2 subfamily.UniRule annotation

Phylogenomic databases

OrthoDBiEOG092C0KHR

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.420.10, 1 hit
HAMAPiMF_03182 PAN2, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR030843 PAN2
IPR028881 PAN2_dom
IPR038765 Papain_like_cys_pep_sf
IPR011047 Quinoprotein_ADH-like_supfam
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR028889 USP_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
PF13423 UCH_1, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF50998 SSF50998, 2 hits
SSF53098 SSF53098, 1 hit
SSF54001 SSF54001, 2 hits
PROSITEiView protein in PROSITE
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

B3LHF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNWQHFFNN PVDLSEHLKK PYFRFDNRDK EITAISFDEK ANLIWSGDSY
60 70 80 90 100
GCISSYDPTF QLYTRYRGHI GGNSVKDILS HRDGILSISE DSLHFANRRG
110 120 130 140 150
VTKLNLTSID IAAFSELNTM CYSPHSLKNN IYCGGDNTNW GIASIDLNRG
160 170 180 190 200
CLDSLLNYSS KVKLMCSNNK VLSIGRQTGT VDLLDPTSNR TIKSFNAHSA
210 220 230 240 250
SISAMDLRDN TLVTVGKSKR FYNLYADPFV NVYDLRTMRQ LPPVSFSKGT
260 270 280 290 300
TMGSGGADFV QLHPLLPTVM IVASSSGSFD FIDLSNPTLR TQYVHPCQSI
310 320 330 340 350
KKLCLSPNGD VLGILEADNH LDTWRRSSNN MGMFTNTPEM LAYPDYFNDI
360 370 380 390 400
TSDGPISVDD ETYPLSSVGM PYYLDKLLSA WPPVVFKSEG TIPQLTGKSP
410 420 430 440 450
LPSSGKLKSN LAVISSQNEK LSTQEFPLLR YDRTKYGMRN AIPDYVCLRD
460 470 480 490 500
IRKQITSGLE TSDIQTYTSI NKYEVPPAYS RLPLTSGRFG TDNFDFTPFN
510 520 530 540 550
NTEYSGLDPD VDNHYTNAII QLYRFIPEMF NFVVGCLKDE NFETTLLTDL
560 570 580 590 600
GYLFDMMERS HGKICSSSNF QASLKSLTDK RQLENGEPQE HLEEYLESLC
610 620 630 640 650
IRESIEDFNS SESIKRNMPQ KFNRFLLSQL IKEEAQTVNH NITLNQCFGL
660 670 680 690 700
ETEIRTECSC DHYDTTVKLL PSLSISGINK TVIKQLNKKS NGQNILPYIE
710 720 730 740 750
YAMKNVTQKN SICPTCGKTE TITQECTVKN LPSVLSLELS LLDTEFSNIR
760 770 780 790 800
SSKNWLTSEF YGSIIKNKAV LRSTASELKG TSHIFKYELN GYVAKITDNN
810 820 830 840 850
NETRLVTYVK KYNPKENCFK WLMFNDYLVV EITEEEALKM TYPWKTPEII
860 870 880 890 900
IYCDAEELRK PFFSVDTYSI NYDILFRDYF ANGIRDTARR EYKLLTHDEA
910 920 930 940 950
PKSGTLVAID AEFVSLQSEL CEIDHQGIRS IIRPKRTALA RISIIRGEEG
960 970 980 990 1000
ELYGVPFVDD YVVNTNHIED YLTRYSGILP GDLDPEKSTK RLVRRNVVYR
1010 1020 1030 1040 1050
KVWLLMQLGC VFVGHGLNND FKHININVPR NQIRDTAIYF LQGKRYLSLR
1060 1070 1080 1090 1100
YLAYVLLGMN IQEGNHDSIE DAHTALILYK KYLHLKEKAI FEKVLNSVYE
1110
EGRAHNFKVP ETSKG
Length:1,115
Mass (Da):127,039
Last modified:September 2, 2008 - v1
Checksum:i4F91B737F761AE5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH408044 Genomic DNA Translation: EDV10317.1

Genome annotation databases

EnsemblFungiiEDV10317; EDV10317; SCRG_01092

Similar proteinsi

Entry informationi

Entry nameiB3LHF9_YEAS1
AccessioniPrimary (citable) accession number: B3LHF9
Entry historyiIntegrated into UniProtKB/TrEMBL: September 2, 2008
Last sequence update: September 2, 2008
Last modified: June 20, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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