UniProtKB - B3KMX0 (B3KMX0_HUMAN)
DNA replication licensing factor MCM4
Functioni
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
UniRule annotationCatalytic activityi
- EC:3.6.4.12UniRule annotationARBA annotation
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ATP hydrolysis activity Source: RHEA
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- DNA replication initiation Source: InterPro
Keywordsi
Molecular function | DNA-bindingUniRule annotationARBA annotation, HelicaseUniRule annotationARBA annotation, Hydrolase |
Biological process | Cell cycleARBA annotation, DNA replicationUniRule annotationARBA annotation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM4UniRule annotation (EC:3.6.4.12UniRule annotation) |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Subcellular locationi
Nucleus
- Nucleus ARBA annotation
Other locations
- Chromosome ARBA annotation
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- chromosome Source: UniProtKB-SubCell
- MCM complex Source: UniProtKB-UniRule
Keywords - Cellular componenti
ChromosomeARBA annotation, NucleusUniRule annotationARBA annotationInteractioni
Subunit structurei
Component of the MCM2-7 complex.
UniRule annotationStructurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 458 – 666 | MCMInterPro annotationAdd BLAST | 209 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 60 | DisorderedSequence analysisAdd BLAST | 60 | |
Regioni | 91 – 126 | DisorderedSequence analysisAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 44 – 60 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
OrthoDBi | 266497at2759 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR031327, MCM IPR008047, MCM_4 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF66, PTHR11630:SF66, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01660, MCMPROTEIN4 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP
60 70 80 90 100
MPTSPGVDLQ SPAAQDVLFS SPPQMHSSAI PLDFDVSSPL TYGTPSSRVE
110 120 130 140 150
GTPRSGVRGT PVRQRPDLGS AQKGLQVDLQ SDGAAAEDIV ASEQSLGQKL
160 170 180 190 200
VIWGTDVNVA ACKENFQRFL QRFIDPLAKE EENVGIDITE PLYMQRLGEI
210 220 230 240 250
NVIGEPFLNV NCEHIKSFDK NLYRQLISYP QEVIPTFDMA VNEIFFDRYP
260 270 280 290 300
DSILEHQIQV RPFNALKTKN MRNLNPEDID QLITISGMVI RTSQLIPEMQ
310 320 330 340 350
EAFFQCQVCA HTTRVEMDRG RIAEPSVCGR CHTTHSMALI HNRSLFSDKQ
360 370 380 390 400
MIKLQESPED MPAGQTPHTV ILFAHNDLVD KVQPGDRVNV TGIYRAVPIR
410 420 430 440 450
VNPRVSNVKS VYKTHIDVIH YRKTDAKRLH GLDEEAEQKL FSEKRVELLK
460 470 480 490 500
ELSRKPDIYE RLASALAPSI YEHEDIKKGI LLQLFGGTRK DFSHTGRGKF
510 520 530 540 550
RAEINILLCG DPGTSKSQLL QYVYNLVPRG QYTSGKGSSA VGLTAYVMKD
560 570 580 590 600
PETRQLVLQT GALVLSDNGI CCIDEFDRMN ESTRSVLHEV MEQQTLSIAK
610 620 630 640 650
AGIICQLNAR TSVLAAANPI ESQWNPKKTT IENIQLPHTL LSRFDLIFLM
660 670 680 690 700
LDPQDEAYDR RLAHHLVALY YQSEEQAEEE LLDMAVLKDY IAYAHSTIMP
710 720 730 740 750
RLSEEASQAL IEAYVDMRKI GSSRGMVSAY PRQLESLIRL AEAHAKVRLS
760 770 780 790 800
NKVEAIDVEE AKRLHREALK QSATDPRTGI VDISILTTGM SATSRKRKEE
810 820 830 840 850
LAEALKKLIL SKGKTPALKY QQLFEDIRGQ SDIAITKDMF EEALRALADD
860
DFLTVTGKTV RLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK022899 mRNA Translation: BAG51132.1 |
RefSeqi | NP_877423.1, NM_182746.2 |
Genome annotation databases
GeneIDi | 4173 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK022899 mRNA Translation: BAG51132.1 |
RefSeqi | NP_877423.1, NM_182746.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PeptideAtlasi | B3KMX0 |
Protocols and materials databases
DNASUi | 4173 |
Genome annotation databases
GeneIDi | 4173 |
Organism-specific databases
CTDi | 4173 |
PharmGKBi | PA30694 |
Phylogenomic databases
OrthoDBi | 266497at2759 |
Miscellaneous databases
BioGRID-ORCSi | 4173, 735 hits in 1058 CRISPR screens |
GenomeRNAii | 4173 |
Gene expression databases
Genevisiblei | B3KMX0, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR031327, MCM IPR008047, MCM_4 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF66, PTHR11630:SF66, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01660, MCMPROTEIN4 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | B3KMX0_HUMAN | |
Accessioni | B3KMX0Primary (citable) accession number: B3KMX0 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | September 2, 2008 |
Last sequence update: | September 2, 2008 | |
Last modified: | January 19, 2022 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |