UniProtKB - B3DLA6 (DICER_XENTR)
Endoribonuclease Dicer
dicer1
Functioni
Catalytic activityi
- Endonucleolytic cleavage to 5'-phosphomonoester. EC:3.1.26.3
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1293 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 1371 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 1374 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 1676 | Magnesium or manganese 2By similarity | 1 | |
Sitei | 1777 | Important for activityBy similarity | 1 | |
Metal bindingi | 1781 | Magnesium or manganese 2By similarity | 1 | |
Metal bindingi | 1784 | Magnesium or manganese 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 54 – 61 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- deoxyribonuclease I activity Source: GO_Central
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: GO_Central
- RNA binding Source: GO_Central
GO - Biological processi
- apoptotic DNA fragmentation Source: GO_Central
- pre-miRNA processing Source: UniProtKB
- production of siRNA involved in RNA interference Source: UniProtKB
- RNA phosphodiester bond hydrolysis Source: GO_Central
- targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
Keywordsi
Molecular function | Endonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | RNA-mediated gene silencing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-XTR-203927, MicroRNA (miRNA) biogenesis R-XTR-426486, Small interfering RNA (siRNA) biogenesis |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dicer1 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-491114, dicer1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- RISC complex Source: UniProtKB
- RISC-loading complex Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000373986 | 1 – 1893 | Endoribonuclease DicerAdd BLAST | 1893 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | B3DLA6 |
PRIDEi | B3DLA6 |
Interactioni
Subunit structurei
Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of dicer1, ago2 and tarbp2; dicer1 and tarbp2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto ago2. Note that the trimeric RLC/miRLC is also referred to as RISC (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 8364.ENSXETP00000050382 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 41 – 217 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 177 | |
Domaini | 424 – 593 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 170 | |
Domaini | 621 – 713 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 892 – 1036 | PAZPROSITE-ProRule annotationAdd BLAST | 145 | |
Domaini | 1249 – 1380 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 132 | |
Domaini | 1637 – 1795 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 159 | |
Domaini | 1820 – 1885 | DRBMPROSITE-ProRule annotationAdd BLAST | 66 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 165 – 168 | DECH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 407 – 412 | Poly-Asp | 6 | |
Compositional biasi | 1106 – 1109 | Poly-Ser | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0701, Eukaryota |
GeneTreei | ENSGT00940000156287 |
InParanoidi | B3DLA6 |
OrthoDBi | 1337630at2759 |
Family and domain databases
CDDi | cd10843, DSRM_DICER, 1 hit cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 1 hit 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR044441, DICER_DSRM IPR014720, dsRBD_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 1 hit SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT
60 70 80 90 100
IVCLNSGSGK TFIAVLLSKE LSYQIRGDFS KNTKRTVFLV NSEKQVSQQV
110 120 130 140 150
SAVRTHTDLK VGEYSDQEKT QCWAKERWYL EFETHQVLVM TCHIFLNVLK
160 170 180 190 200
SGNVSLSNIN LLVFDECHLA IQDHPYREIM KICESCQPCP RILGLTASIL
210 220 230 240 250
NGKCDPRDLE EKIQKLEEIL RSNAETATDL VVLDRYASQP CEIVLDCGPY
260 270 280 290 300
IDKSGLYQRL LNELDEALNF LIDCNISTHS KERDSTLISK QILSDCQTVL
310 320 330 340 350
LVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT ILRKIHALCE
360 370 380 390 400
EHFSPASLDM KFVTPKVIKL LEILRKYKPY ERQQFESVEW YNNRNQDNYV
410 420 430 440 450
SWSDSEDDDD EDEEIEEKEK TETSFPSPFT NILCGIIFVE RRYTAVVLNR
460 470 480 490 500
LIKEAGKQDP ELAYISSNFI TGHGIGKNQP RNKQMEVEFR KQEEVLRKFR
510 520 530 540 550
AHETNLLIAT SIVEEGVDIP KCNLVVRFDL PSEYRSYVQS KGRARAPISN
560 570 580 590 600
YIMLADSDKI KAFEEDLKTY KAIEKILRNK CSKSIDCGNT ESEPIVDDDE
610 620 630 640 650
IFPPYVLRQD DGSPRVTINT AIGHINRYCA RLPSDPFTHL APKCKTREFP
660 670 680 690 700
DGLYRSTLYL PINSPLRAPI VGPPMNCGRL ADRAVALICC KKLHEIGELD
710 720 730 740 750
DHLMPVGKET VKYEEELDLH DEEETSVPGR PGSTKRRQCY PKAIPECLRN
760 770 780 790 800
SYPKPGQPCY LYVIGMVLTT PLPDELNFRR RKLYPPEDTT RCFGILTAKP
810 820 830 840 850
IPQIPHFPVY TRSGEVTISI ELKKSGFTLN LEQLELITRL HQYIFSHILR
860 870 880 890 900
LEKPALEFKP TVADCAYCVL PLNVVNDSGT LDIDFKFVED IEKSEARTGI
910 920 930 940 950
PNTQYSAESP FIFKLEDYQD AVIIPRQVIY RNFDQPHRFY VADVYTDLTP
960 970 980 990 1000
LSKFPSPEYE TFAEYYKTKY NLDLTNLNQP LLDVDHTSSR LNLLTPRHLN
1010 1020 1030 1040 1050
QKGKALPLSS AEKRKAKWES LQNKQILVPE LCAIHPVPAS LWRKAVCLPS
1060 1070 1080 1090 1100
ILYRLHCLLT AEELRAQTAI DAGVGVKSLP DDFRYPNLDF GWKRSIDSKT
1110 1120 1130 1140 1150
FISNQSSSSV ESESDCRLNK TTAPDSAASS AANSVIYMQI NDQMSVNCTP
1160 1170 1180 1190 1200
PCQKSLSHLQ TVCFSDDYKA INGISCNGLT NGDWEAESAA CFQKDERITC
1210 1220 1230 1240 1250
KQEIPEKSTS FHVQNLPKEN QPILKECTLS NSDGNVSKPT SDECPSTCTS
1260 1270 1280 1290 1300
DMHYDSGLSN RHSSKTLGPN PGLILQALTL SNASDGFNLE RLEMLGDSFL
1310 1320 1330 1340 1350
KHAITTYLFC TYPDAHEGRL SYMRSKKVSN CNLYRLGKKK GSPSRMVVSI
1360 1370 1380 1390 1400
FDPPVNWLPP GYIVNQDKNS DKWESNETSG EDVMVNGKID EDFDDEEDED
1410 1420 1430 1440 1450
LMWRNPKEET DFDDDFLEYD QEHIKFIDSM LMGSGAFVKK IPLSSFAPPD
1460 1470 1480 1490 1500
QNYEWRAPKK PPLESSQFPC DFDDFDYSSW DAMCYLDPSK AVEEDDFVVG
1510 1520 1530 1540 1550
FWNPSEENCG ADAGKQSISY DLHTEQCIAD KSIADCVEAL LGCYLTSCGE
1560 1570 1580 1590 1600
RAAQLFLCSL GLKVLPEVRK LVTNTNVISA SSSYQNSTRD NCTLTARTNT
1610 1620 1630 1640 1650
DLSSCKGIDY GYLKIPPRCM FEHPDAEKTL DHLISGFENF EKKINYPFKN
1660 1670 1680 1690 1700
KAYLLQAFTH ASYHYNTITD CYQRLEFLGD AILDYLITKH LYEDPRQHSP
1710 1720 1730 1740 1750
GVLTDLRSAL VNNTIFASLA VKYDYHKYFK AISPELFHVI DDFVQFQLEK
1760 1770 1780 1790 1800
NEMQGMDSEL RRSEEDEEKE EDIEVPKAMG DIFESLAGAI YMDSGMSLET
1810 1820 1830 1840 1850
VWHVYYPMMQ PLIEKFSANV PRSPVRELLE MEPETAKFSP AERTYDGKVR
1860 1870 1880 1890
VTVEVVGKGK FKGVGRSYRI AKSAAARRAL RSLKANQSQV PNS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6SRY9 | F6SRY9_XENTR | Endoribonuclease Dicer | dicer1 | 1,899 | Annotation score: | ||
A0A5S6NLR8 | A0A5S6NLR8_XENTR | Endoribonuclease Dicer | cdc42se2 dicer1 | 1,884 | Annotation score: | ||
A0A6I8QWI0 | A0A6I8QWI0_XENTR | Endoribonuclease Dicer | dicer1 | 1,884 | Annotation score: | ||
A0A6I8S605 | A0A6I8S605_XENTR | Endoribonuclease Dicer | dicer1 | 1,841 | Annotation score: | ||
A0A6I8R5E1 | A0A6I8R5E1_XENTR | Endoribonuclease Dicer | dicer1 | 1,733 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037298 | 804 – 811 | IPHFPVYT → VLQCTIKF in isoform 2. 1 Publication | 8 | |
Alternative sequenceiVSP_037299 | 812 – 1893 | Missing in isoform 2. 1 PublicationAdd BLAST | 1082 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU338242 mRNA Translation: ACA52289.1 BC167373 mRNA Translation: AAI67373.1 |
RefSeqi | NP_001123390.2, NM_001129918.2 [B3DLA6-1] |
Genome annotation databases
Ensembli | ENSXETT00000096069; ENSXETP00000082776; ENSXETG00000023315 [B3DLA6-1] |
GeneIDi | 100170154 |
KEGGi | xtr:100170154 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU338242 mRNA Translation: ACA52289.1 BC167373 mRNA Translation: AAI67373.1 |
RefSeqi | NP_001123390.2, NM_001129918.2 [B3DLA6-1] |
3D structure databases
SMRi | B3DLA6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000050382 |
Proteomic databases
PaxDbi | B3DLA6 |
PRIDEi | B3DLA6 |
Genome annotation databases
Ensembli | ENSXETT00000096069; ENSXETP00000082776; ENSXETG00000023315 [B3DLA6-1] |
GeneIDi | 100170154 |
KEGGi | xtr:100170154 |
Organism-specific databases
CTDi | 23405 |
Xenbasei | XB-GENE-491114, dicer1 |
Phylogenomic databases
eggNOGi | KOG0701, Eukaryota |
GeneTreei | ENSGT00940000156287 |
InParanoidi | B3DLA6 |
OrthoDBi | 1337630at2759 |
Enzyme and pathway databases
Reactomei | R-XTR-203927, MicroRNA (miRNA) biogenesis R-XTR-426486, Small interfering RNA (siRNA) biogenesis |
Gene expression databases
ExpressionAtlasi | B3DLA6, baseline |
Family and domain databases
CDDi | cd10843, DSRM_DICER, 1 hit cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 1 hit 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR044441, DICER_DSRM IPR014720, dsRBD_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 1 hit SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DICER_XENTR | |
Accessioni | B3DLA6Primary (citable) accession number: B3DLA6 Secondary accession number(s): C0IN01 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 26, 2009 |
Last sequence update: | May 26, 2009 | |
Last modified: | April 7, 2021 | |
This is version 82 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families