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UniProtKB - B2ZGJ1 (CLAT_DANRE)
Protein
Choline O-acetyltransferase
Gene
chat
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
1 PublicationCatalytic activityi
- EC:2.3.1.6
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 329 | Proton acceptorBy similarity | 1 | |
Binding sitei | 445 | Coenzyme ABy similarity | 1 | |
Binding sitei | 545 | Coenzyme ABy similarity | 1 |
GO - Molecular functioni
- choline O-acetyltransferase activity Source: GO_Central
GO - Biological processi
- acetylcholine biosynthetic process Source: GO_Central
- locomotory behavior Source: UniProtKB
- neuromuscular synaptic transmission Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Neurotransmitter biosynthesis |
Enzyme and pathway databases
BRENDAi | 2.3.1.6, 928 |
Reactomei | R-DRE-1483191, Synthesis of PC R-DRE-264642, Acetylcholine Neurotransmitter Release Cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Choline O-acetyltransferase (EC:2.3.1.6)Short name: CHOACTase Short name: ChAT Short name: Choline acetylase |
Gene namesi | Name:chat |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-080102-2, chata |
Subcellular locationi
Other locations
- cytoplasm Source: GO_Central
- neuron projection Source: GO_Central
- synapse Source: GOC
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000351550 | 1 – 637 | Choline O-acetyltransferaseAdd BLAST | 637 |
Proteomic databases
PaxDbi | B2ZGJ1 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000015854, Expressed in retina and 14 other tissues |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 407 – 419 | Coenzyme A bindingBy similarityAdd BLAST | 13 |
Sequence similaritiesi
Belongs to the carnitine/choline acetyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG3717, Eukaryota |
GeneTreei | ENSGT01040000240407 |
HOGENOMi | CLU_013513_3_0_1 |
InParanoidi | B2ZGJ1 |
OMAi | MAYRDPV |
OrthoDBi | 559299at2759 |
PhylomeDBi | B2ZGJ1 |
TreeFami | TF313836 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 3.30.559.70, 1 hit |
InterProi | View protein in InterPro IPR000542, Carn_acyl_trans IPR023213, CAT-like_dom_sf IPR039551, Cho/carn_acyl_trans IPR042231, Cho/carn_acyl_trans_2 |
PANTHERi | PTHR22589, PTHR22589, 1 hit |
Pfami | View protein in Pfam PF00755, Carn_acyltransf, 1 hit |
PROSITEi | View protein in PROSITE PS00439, ACYLTRANSF_C_1, 1 hit PS00440, ACYLTRANSF_C_2, 1 hit |
i Sequence
Sequence statusi: Complete.
B2ZGJ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPVSKREQSK DTGDPCALPK LPIPPLKQTL DMYLTCMGHL VPEDQFRKTK
60 70 80 90 100
AVVEKFGAPG GVGETLQKKL LERSEQKANW VYDYWLEDMY LNNRLALPVN
110 120 130 140 150
SSPVMVFHKQ NFKGQSDVLR FAANLISGVL EYKALIDGRA LPVEHARGQL
160 170 180 190 200
AGTPLCMDQY NKVFTSYRLP GTKTDTLVAQ KSTVMPEPEH IIVACKNQFF
210 220 230 240 250
VLDVMVNFRR LNEKDLYTQL ERIRKMADIE EERQPPIGLL TSDGRTQWAE
260 270 280 290 300
ARNILIKDST NRDSLDMIER CLCLVCLDEE TATELNDSNR ALLMLHGGGT
310 320 330 340 350
DKNGGNRWYD KPMQFVIGAD GCCGVVCEHS PFEGIVLVQC SEYLLRYMRG
360 370 380 390 400
SPSKLVRAAS MSELPAPRRL RWKCSPDIQT FLSASADRLQ KLVKNLDMNV
410 420 430 440 450
HKFTGYGKEF IKRQKMSPDA YVQVALQFTF YRCHGRLVPT YESASIRRFQ
460 470 480 490 500
EGRVDNIRSS TPEALAFVKA MASGSKITDA EKMELLWTAI KAQTNYTILA
510 520 530 540 550
ITGMAIDNHL LGLREIAKEL KLEKPELFSD TTYATSIHFT LSTSQVPTTE
560 570 580 590 600
EMFCCYGPVV PNGYGACYNP QTDHILFCVS SFRECAETSS DLFVKTLEGC
610 620 630
LKEMQDLCRK CNTEVKPADS TQRMEGNPKV MKNGSKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU660883 mRNA Translation: ACD40042.1 |
RefSeqi | NP_001124191.1, NM_001130719.1 XP_005156786.1, XM_005156729.3 |
Genome annotation databases
Ensembli | ENSDART00000024225; ENSDARP00000025508; ENSDARG00000015854 ENSDART00000150228; ENSDARP00000125038; ENSDARG00000015854 |
GeneIDi | 100170938 |
KEGGi | dre:100170938 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU660883 mRNA Translation: ACD40042.1 |
RefSeqi | NP_001124191.1, NM_001130719.1 XP_005156786.1, XM_005156729.3 |
3D structure databases
AlphaFoldDBi | B2ZGJ1 |
SMRi | B2ZGJ1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000125038 |
Proteomic databases
PaxDbi | B2ZGJ1 |
Genome annotation databases
Ensembli | ENSDART00000024225; ENSDARP00000025508; ENSDARG00000015854 ENSDART00000150228; ENSDARP00000125038; ENSDARG00000015854 |
GeneIDi | 100170938 |
KEGGi | dre:100170938 |
Organism-specific databases
CTDi | 100170938 |
ZFINi | ZDB-GENE-080102-2, chata |
Phylogenomic databases
eggNOGi | KOG3717, Eukaryota |
GeneTreei | ENSGT01040000240407 |
HOGENOMi | CLU_013513_3_0_1 |
InParanoidi | B2ZGJ1 |
OMAi | MAYRDPV |
OrthoDBi | 559299at2759 |
PhylomeDBi | B2ZGJ1 |
TreeFami | TF313836 |
Enzyme and pathway databases
BRENDAi | 2.3.1.6, 928 |
Reactomei | R-DRE-1483191, Synthesis of PC R-DRE-264642, Acetylcholine Neurotransmitter Release Cycle |
Miscellaneous databases
PROi | PR:B2ZGJ1 |
Gene expression databases
Bgeei | ENSDARG00000015854, Expressed in retina and 14 other tissues |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 3.30.559.70, 1 hit |
InterProi | View protein in InterPro IPR000542, Carn_acyl_trans IPR023213, CAT-like_dom_sf IPR039551, Cho/carn_acyl_trans IPR042231, Cho/carn_acyl_trans_2 |
PANTHERi | PTHR22589, PTHR22589, 1 hit |
Pfami | View protein in Pfam PF00755, Carn_acyltransf, 1 hit |
PROSITEi | View protein in PROSITE PS00439, ACYLTRANSF_C_1, 1 hit PS00440, ACYLTRANSF_C_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CLAT_DANRE | |
Accessioni | B2ZGJ1Primary (citable) accession number: B2ZGJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 14, 2008 |
Last sequence update: | July 1, 2008 | |
Last modified: | May 25, 2022 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families