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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Sulfurihydrogenibium sp. (strain YO3AOP1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Active sitei128Proton acceptorUniRule annotation1
Binding sitei155Allosteric activator ADPUniRule annotation1
Binding sitei163Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei223SubstrateUniRule annotation1
Binding sitei248Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSP436114:G1GC2-423-MONOMER
UniPathwayi
UPA00109;UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:SYO3AOP1_0397
OrganismiSulfurihydrogenibium sp. (strain YO3AOP1)
Taxonomic identifieri436114 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaeSulfurihydrogenibium
Proteomesi
  • UP000001201 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001332891 – 324ATP-dependent 6-phosphofructokinaseAdd BLAST324

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi436114.SYO3AOP1_0397

Structurei

3D structure databases

ProteinModelPortaliB2V7W7
SMRiB2V7W7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 25Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni126 – 128Substrate bindingUniRule annotation3
Regioni170 – 172Substrate bindingUniRule annotation3
Regioni186 – 188Allosteric activator ADP bindingUniRule annotation3
Regioni214 – 216Allosteric activator ADP bindingUniRule annotation3
Regioni254 – 257Substrate bindingUniRule annotation4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiGKLHSII
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

B2V7W7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKIGLLTSG GDCPGLNACI RAVVRTANYY NIEVVAFKRG FKGLIENDFT
60 70 80 90 100
TLDYKSVAGI LQKGGTILLT AREPRFKDYN FRKIAYENIQ KHNIEALFVI
110 120 130 140 150
GGNGSFQGAY LLQKDFGLNI IGIPKTIDND IYGTDYAIGF DTAVNNAMEA
160 170 180 190 200
IDKIKDTTMS HERIFIVEVM GRDNGFIALE VGIAVGAELT LIPEYPFPLH
210 220 230 240 250
VIEETILKAK EMGKNFAIIV LAEGVASAKE LSEILNERLK DKDVGEIRYQ
260 270 280 290 300
VLGYIQRGGS PSAYDRVMAS KFGVFAVEKF VQGEKNFMVA YENGKLLTKP
310 320
LEISFNKVRI PNLEEYQINN ILSM
Length:324
Mass (Da):36,049
Last modified:July 1, 2008 - v1
Checksum:iAFFFE32028C29772
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001080 Genomic DNA Translation: ACD66040.1
RefSeqiWP_012459122.1, NC_010730.1

Genome annotation databases

EnsemblBacteriaiACD66040; ACD66040; SYO3AOP1_0397
KEGGisul:SYO3AOP1_0397

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001080 Genomic DNA Translation: ACD66040.1
RefSeqiWP_012459122.1, NC_010730.1

3D structure databases

ProteinModelPortaliB2V7W7
SMRiB2V7W7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi436114.SYO3AOP1_0397

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD66040; ACD66040; SYO3AOP1_0397
KEGGisul:SYO3AOP1_0397

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
KOiK00850
OMAiGKLHSII
OrthoDBiPOG091H01AC

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00182

BioCyciSSP436114:G1GC2-423-MONOMER

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_SULSY
AccessioniPrimary (citable) accession number: B2V7W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 1, 2008
Last modified: November 7, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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