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Protein

TAL effector protein PthXo1

Gene

pthXo1

Organism
Xanthomonas oryzae pv. oryzae (strain PXO99A)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Avirulence protein. Acts as a transcription factor in rice, inducing expression of a number of host genes including SWEET11 (Os8N3, XA13, AC Q6YZF3) in susceptible plants with the Xa13 allele. Plants with the xa13 allele, which has an altered promoter, are resistant to bacterial blight caused by this bacterial strain and do not induce SWEET11. The xa13 allele elicits an atypical hypersensitive response (HR). PthXo1 binds SWEET11 promoter DNA in a sequence-specific manner.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processHypersensitive response elicitation, Transcription, Transcription regulation, Virulence

Enzyme and pathway databases

BioCyciXORY360094:G1GBE-1589-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
TAL effector protein PthXo11 Publication
Alternative name(s):
Avirulence protein PthXo11 Publication
TAL effector protein Tal2b1 Publication
Gene namesi
Name:pthXo1
Synonyms:tal2b
Ordered Locus Names:PXO_00227
OrganismiXanthomonas oryzae pv. oryzae (strain PXO99A)
Taxonomic identifieri360094 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001740 Componenti: Chromosome

Subcellular locationi

  • Secreted By similarity
  • Host nucleus By similarity
  • Note: Secreted via a type III secretions system (TTSS). Localizes to the plant cell nucleus.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Secreted

Pathology & Biotechi

Biotechnological usei

By combining the central DNA-binding domain with the catalytic domain of the restriction endonuclease FokI, TALE-nuclease (TALEN) enzymes able to target specific dsDNA sequences can be created that enable eukaryotic genome modification. Other potential uses as transcriptional repressors, for transposon targeting, DNA methylation or histone tail modifictions are also possible.2 Publications1 Publication

Disruption phenotypei

Reduced virulence in susceptible rice; its introduction into a deletion mutant restores virulence to the bacteria in rice plants. No longer induces SWEET11 (Os8N3, XA13, AC Q6YZF3).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004306211 – 1373TAL effector protein PthXo1Add BLAST1373

Structurei

Secondary structure

11373
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiB2SU53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati221 – 239Cryptic repeat -11 PublicationAdd BLAST19
Repeati256 – 273Cryptic repeat 01 PublicationAdd BLAST18
Repeati289 – 322Core repeat 11 PublicationAdd BLAST34
Repeati323 – 356Core repeat 21 PublicationAdd BLAST34
Repeati357 – 390Core repeat 31 PublicationAdd BLAST34
Repeati391 – 424Core repeat 41 PublicationAdd BLAST34
Repeati425 – 458Core repeat 51 PublicationAdd BLAST34
Repeati459 – 492Core repeat 61 PublicationAdd BLAST34
Repeati493 – 525Core repeat 71 PublicationAdd BLAST33
Repeati526 – 559Core repeat 81 PublicationAdd BLAST34
Repeati560 – 593Core repeat 91 PublicationAdd BLAST34
Repeati594 – 627Core repeat 101 PublicationAdd BLAST34
Repeati628 – 661Core repeat 111 PublicationAdd BLAST34
Repeati662 – 695Core repeat 121 PublicationAdd BLAST34
Repeati696 – 729Core repeat 131 PublicationAdd BLAST34
Repeati714 – 760HEAT 1Sequence analysisAdd BLAST47
Repeati730 – 763Core repeat 141 PublicationAdd BLAST34
Repeati764 – 797Core repeat 151 PublicationAdd BLAST34
Repeati782 – 828HEAT 2Sequence analysisAdd BLAST47
Repeati798 – 831Core repeat 161 PublicationAdd BLAST34
Repeati832 – 865Core repeat 171 PublicationAdd BLAST34
Repeati850 – 893HEAT 3Sequence analysisAdd BLAST44
Repeati866 – 899Core repeat 181 PublicationAdd BLAST34
Repeati900 – 933Core repeat 191 PublicationAdd BLAST34
Repeati918 – 961HEAT 4Sequence analysisAdd BLAST44
Repeati934 – 967Core repeat 201 PublicationAdd BLAST34
Repeati968 – 1001Core repeat 211 PublicationAdd BLAST34
Repeati1002 – 1034Core repeat 221 PublicationAdd BLAST33
Repeati1035 – 1068Core repeat 231 PublicationAdd BLAST34
Repeati1053 – 1091HEAT 5Sequence analysisAdd BLAST39
Repeati1069 – 1087Core repeat 23.51 PublicationAdd BLAST19

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1136 – 1364Acidic activation domainBy similarityAdd BLAST229

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1222 – 1225Nuclear localization signal NLS1By similarity4
Motifi1268 – 1271Nuclear localization signal NLS2By similarity4
Motifi1305 – 1308Nuclear localization signal NLS3By similarity4

Domaini

The central DNA-binding region is composed of 23.5 tandem core repeats with 1 base-specifying residue (BSR residue 13, also called the repeat variable diresidue, RVD, residues 12 and 13) each of which recognizes 1 base in the target DNA. The BSR is the only residue which contacts DNA in a sequence-specific manner.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000060719
KOiK18876

Family and domain databases

InterProiView protein in InterPro
IPR005042 TAL_effector_rpt
PfamiView protein in Pfam
PF03377 TAL_effector, 24 hits

Sequencei

Sequence statusi: Complete.

B2SU53-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDPIRSRTPS PARELLPGPQ PDRVQPTADR GGAPPAGGPL DGLPARRTMS
60 70 80 90 100
RTRLPSPPAP SPAFSAGSFS DLLRQFDPSL LDTSLLDSMP AVGTPHTAAA
110 120 130 140 150
PAECDEVQSG LRAADDPPPT VRVAVTAARP PRAKPAPRRR AAQPSDASPA
160 170 180 190 200
AQVDLRTLGY SQQQQEKIKP KVGSTVAQHH EALVGHGFTH AHIVALSRHP
210 220 230 240 250
AALGTVAVKY QDMIAALPEA THEDIVGVGK QWSGARALEA LLTVAGELRG
260 270 280 290 300
PPLQLDTGQL VKIAKRGGVT AVEAVHASRN ALTGAPLNLT PAQVVAIASN
310 320 330 340 350
NGGKQALETV QRLLPVLCQA HGLTPAQVVA IASHDGGKQA LETMQRLLPV
360 370 380 390 400
LCQAHGLPPD QVVAIASNIG GKQALETVQR LLPVLCQAHG LTPDQVVAIA
410 420 430 440 450
SHGGGKQALE TVQRLLPVLC QAHGLTPDQV VAIASHDGGK QALETVQRLL
460 470 480 490 500
PVLCQAHGLT PDQVVAIASN GGGKQALETV QRLLPVLCQA HGLTPDQVVA
510 520 530 540 550
IASNGGKQAL ETVQRLLPVL CQAHGLTPDQ VVAIASHDGG KQALETVQRL
560 570 580 590 600
LPVLCQTHGL TPAQVVAIAS HDGGKQALET VQQLLPVLCQ AHGLTPDQVV
610 620 630 640 650
AIASNIGGKQ ALATVQRLLP VLCQAHGLTP DQVVAIASNG GGKQALETVQ
660 670 680 690 700
RLLPVLCQAH GLTPDQVVAI ASNGGGKQAL ETVQRLLPVL CQAHGLTQVQ
710 720 730 740 750
VVAIASNIGG KQALETVQRL LPVLCQAHGL TPAQVVAIAS HDGGKQALET
760 770 780 790 800
VQRLLPVLCQ AHGLTPDQVV AIASNGGGKQ ALETVQRLLP VLCQAHGLTQ
810 820 830 840 850
EQVVAIASNN GGKQALETVQ RLLPVLCQAH GLTPDQVVAI ASNGGGKQAL
860 870 880 890 900
ETVQRLLPVL CQAHGLTPAQ VVAIASNIGG KQALETVQRL LPVLCQDHGL
910 920 930 940 950
TLAQVVAIAS NIGGKQALET VQRLLPVLCQ AHGLTQDQVV AIASNIGGKQ
960 970 980 990 1000
ALETVQRLLP VLCQDHGLTP DQVVAIASNI GGKQALETVQ RLLPVLCQDH
1010 1020 1030 1040 1050
GLTLDQVVAI ASNGGKQALE TVQRLLPVLC QDHGLTPDQV VAIASNSGGK
1060 1070 1080 1090 1100
QALETVQRLL PVLCQDHGLT PNQVVAIASN GGKQALESIV AQLSRPDPAL
1110 1120 1130 1140 1150
AALTNDHLVA LACLGGRPAM DAVKKGLPHA PELIRRVNRR IGERTSHRVA
1160 1170 1180 1190 1200
DYAQVVRVLE FFQCHSHPAY AFDEAMTQFG MSRNGLVQLF RRVGVTELEA
1210 1220 1230 1240 1250
RGGTLPPASQ RWDRILQASG MKRAKPSPTS AQTPDQASLH AFADSLERDL
1260 1270 1280 1290 1300
DAPSPMHEGD QTGASSRKRS RSDRAVTGPS AQHSFEVRVP EQRDALHLPL
1310 1320 1330 1340 1350
SWRVKRPRTR IGGGLPDPGT PIAADLAASS TVMWEQDAAP FAGAADDFPA
1360 1370
FNEEELAWLM ELLPQSGSVG GTI
Length:1,373
Mass (Da):143,535
Last modified:October 16, 2013 - v2
Checksum:iC96C8A8A862A8217
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti358P → T in AAS46025 (PubMed:15553245).Curated1
Sequence conflicti1120M → L in AAS46025 (PubMed:15553245).Curated1
Sequence conflicti1137V → I in AAS46025 (PubMed:15553245).Curated1
Sequence conflicti1142G → P in AAS46025 (PubMed:15553245).Curated1
Sequence conflicti1150 – 1154ADYAQ → PDLAH in AAS46025 (PubMed:15553245).Curated5
Sequence conflicti1160 – 1164EFFQC → GFFQS in AAS46025 (PubMed:15553245).Curated5
Sequence conflicti1170 – 1174YAFDE → QAFDD in AAS46025 (PubMed:15553245).Curated5
Sequence conflicti1184N → H in AAS46025 (PubMed:15553245).Curated1
Sequence conflicti1198 – 1202LEARG → FEARY in AAS46025 (PubMed:15553245).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000967 Genomic DNA Translation: ACD58243.2
AY495676 Genomic DNA Translation: AAS46025.1
RefSeqiWP_041182630.1, NC_010717.2

Genome annotation databases

EnsemblBacteriaiACD58243; ACD58243; PXO_00227
GeneIDi34179219
KEGGixop:PXO_00227

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000967 Genomic DNA Translation: ACD58243.2
AY495676 Genomic DNA Translation: AAS46025.1
RefSeqiWP_041182630.1, NC_010717.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UGMX-ray3.00A127-1149[»]
SMRiB2SU53
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD58243; ACD58243; PXO_00227
GeneIDi34179219
KEGGixop:PXO_00227

Phylogenomic databases

HOGENOMiHOG000060719
KOiK18876

Enzyme and pathway databases

BioCyciXORY360094:G1GBE-1589-MONOMER

Family and domain databases

InterProiView protein in InterPro
IPR005042 TAL_effector_rpt
PfamiView protein in Pfam
PF03377 TAL_effector, 24 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPTHX1_XANOP
AccessioniPrimary (citable) accession number: B2SU53
Secondary accession number(s): Q6RKD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: October 16, 2013
Last modified: November 7, 2018
This is version 52 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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